GEO DataSets

Analysis of keratinocyte HaCaT cells at 24 and 72 hours following induction of GLI1 or a constitutively active form of GLI2 from a tetracycline inducible promoter. GLI transcription factors mediate the hedgehog signal in development and carcinogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 cell line, 2 time sets

Platform:

GPL1260

Series:

GSE1434

32 Samples

Download data

(Submitter supplied) hybridisation of mRNA from HaCaT keratinocytes expressing GLI1 or GLI2 in an tet-inducible manner for 24h or 72h on Incyte clone set Detailed information about the protocols we used for array production, hybridisation and analysation and the biological background of our experiments can be found in our publications: PMID: 15140221, 14691458, 12165851 Keywords = GLI1/2 target genes Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1974

Platform:

GPL1260

32 Samples

Download data: XLS

(Submitter supplied) Excessive Hedgehog signaling in chondrocytes is sufficient to cause formation of enchondroma-like lesions in mice which can progress to chondrosarcoma. To elucidate potential mechanisms through which activation of Hedgehog signaling contributes to cartilage tumor formation, we used chromatin immunoprecipitation and next generation sequencing to identify Gli1 and Gli2 target genes in primary human chondrosarcoma. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

3 Samples

Download data: BW

(Submitter supplied) ZNF521 is a transcription co-factor with recognized regulatory functions in haematopoietic, osteo-adipogenic and neural progenitor cells. Among its diverse activities, ZNF521 has been implicated in the regulation of medulloblastoma (MB) cells, where the Hedgehog (HH) pathway, has a key role in the development of normal cerebellum and of a substantial fraction of MBs. Here a functional cross-talk is shown for ZNF521 with the HH pathway, where it interacts with GLI1 and GLI2, the major HH transcriptional effectors and enhances the activity of HH signalling. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

4 Samples

Download data: TXT

(Submitter supplied) Aberrant activation of Hedgehog (HH) signaling has been identified as a key etiologic factor of many human malignancies. Signal strength, target gene specificity, and oncogenic activity of HH signaling profoundly depend on interactions with other pathways such as epidermal growth factor receptor-mediated signaling which has been shown to cooperate with HH/GLI in basal cell carcinoma and pancreatic cancer. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL10558

180 Samples

Download data: TXT

(Submitter supplied) Comparison of gene expression changes in Ptch-deficient mouse medulloblastoma cell line SmoWT-MB upon treatment with SMOi GDC-0449 or BETi JQ1. The hypothesis is that they can both inhibit the expressions of Hh signaling target genes in this Hh-driven tumor cell line.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL18752

6 Samples

Download data: TXT

(Submitter supplied) High levels of GLI (GLI1 and GLI2) mRNA and GLI luciferase reporter activity were detected in the androgen independent prostate cancer cell lines DU145 and PC-3 compared to the androgen-dependent LNCaP prostate cancer cell line. Subsequently, we observed that ectopic GLI1 promoted hormone independence in LNCaP cells (LNCaP-GLI1). We compared the gene expression profile of LNCaP-pBP (empty vector), LNCaP-GLI1, DU145, and PC-3 cells globally as well as to identify GLI1-regulated genes that may contribute to hormone independence.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

12 Samples

Download data: TXT