GEO DataSets

(Submitter supplied) This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS686

Platform:

GPL96

22 Samples

Download data

Comparison of HEK-293-derived monoclonal cell lines with high or low levels of store-operated Ca2+ entry (SOCE). Levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 9 cell line, 3 other sets

Platform:

GPL96

Series:

GSE1309

22 Samples

Download data

(Submitter supplied) B lymphoblast samples from control individual, Scott syndrome patient and Scott syndrome daughter. From each samples one part was treated with A23187 ca2+ ionophore. Treated and non-treated samples were then cultured further. Each sample was then processed in duplicate for hybridization on the microarray Keywords: ordered

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1320

Platform:

GPL8300

12 Samples

Download data

Analysis of Scott B lymphoblasts after calcium ionophore A23187 treatment. Lymphoblasts derived from a patient with Scott syndrome, a hemorrhagic disease. Results provide insight into the mechanisms underlying the apoptotic effect of A23187, and susceptibility of Scott B lymphoblasts to apoptosis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 disease state, 3 individual sets

Platform:

GPL8300

Series:

GSE1028

12 Samples

Download data

(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) The Madin-Darby bovine kidney epithelial cells, when at approximately 50% confluence (during the exponential phase), were treated with 10mM sodium butyrate for 24h. Butyrate induced profound changes in gene expression related with multiple signal transduction pathways such as cell cycle control, apoptosis and extracellular matrix remodeling in this cell line Keywords: stress response, two class unpaired design

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3301

6 Samples

Download data

(Submitter supplied) Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

8 Samples

Download data: TXT

(Submitter supplied) Background NASP (Nuclear Autoantigenic Sperm Protein) is a linker histone chaperone required for normal cell division. Changes in NASP expression significantly affect cell growth and development; loss of gene function results in embryonic lethality. However, the mechanism by which NASP exerts its effects in the cell cycle is not understood. To understand the pathways and networks that may involve NASP function, we evaluated gene expression in HeLa cells in which NASP was either overexpressed or inhibited by siRNA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

8 Samples

Download data: TXT

(Submitter supplied) Genomic profile of transporters and ion channels in differentiated or undifferentiated Caco-2 cells grown for 5 days, 1 week, 2 weeks or 3 weeks in flasks or filters Keywords: ordered

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1993

Platform:

GPL1213

32 Samples

Download data

Analysis of colon carcinoma Caco-2 cells cultured for various lengths of time up to 3 weeks in flasks or on filters. An array focusing on transporters and ion channels was used. Results provide insight into the influence of cell differentiation on the mRNA expression of transporters and channels.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 protocol, 4 time sets

Platform:

GPL1213

Series:

GSE1368

32 Samples

Download data