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| Status |
Public on Jun 08, 2004 |
| Title |
Gene expession profiles for monoclonal cell lines with high or low levels of store-operated Ca2+ entry. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip. Two parallel hybridizations were done for each RNA preparation from each monoclonal cell line or from the parental HEK-293 cell culture.
Keywords: parallel sample
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| Contributor(s) |
Zagranichnaya TK, Wu X, Danos A, Villereal ML |
| Citation(s) |
15623568 |
| |
| Submission date |
Apr 14, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Tatiana Zagranichnaya |
| E-mail(s) |
[email protected]
|
| Phone |
773-702-9337
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| Organization name |
The University of Chicago
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| Department |
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| Street address |
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA89905 |
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