GEO DataSets

(Submitter supplied) Murine pancreatic beta cell line MIN6 was growth at two different concentrations of glucose (22,2 and 5,5 mM of glucose), 37ºC, 5% CO2 and was treated at four different concentrations of human amylin (0, 1, 10 and 20 uM) during three different times (2, 12 and 24 hours) Keywords = pancreatic beta cell Keywords = amylin Keywords = glucose Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2945

Platform:

GPL339

20 Samples

Download data: CEL, EXP, RPT

Analysis of MIN6 pancreatic beta-cells treated for up to 24 hours with various concentrations of human islet amyloid polypeptide (IAPP). IAPP aggregration contributes to the development of islet amyloidosis in type 2 diabetes. IAPP oligomers are associated with pancreatic beta-cell apoptosis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 4 dose, 2 growth protocol, 4 time sets

Platform:

GPL339

Series:

GSE2253

20 Samples

Download data: CEL, EXP, RPT

(Submitter supplied) 4-phenylbutyrate (PBA) is a chemical chaperone that has been shown to ameliorate β-cell dysfunction associated with hIAPP overexpression. The aim of the study was to investigate the in vivo effects of PBA in transgenic mice overexpressing hIAPP. We used microarrays to determine gene expression changes in pancreatic islets from mice overexepressing hIAPP and treated with PBA during 12 weeks.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

8 Samples

Download data: CEL

(Submitter supplied) The purpose of this experiment was to determine changes in gene expression by bone marrow-derived macrophages (BMDMs) treated with synthetic human vs. rodent islet amyloid polypeptide (IAPP). Synthetic human IAPP at 15 uM aggregates to form fibrils in vitro, whereas rodent IAPP is non-amyloidogenic. We hypothesized that interaction of macrophages with human IAPP aggregates can activate pro-inflammatory signalling pathways in macrophages, as described for other amyloidogenic peptides.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

8 Samples

Download data: TXT

(Submitter supplied) Strategies to enhance islet b-cell survival and regeneration while refraining inflammation through manipulation of molecular targets would provide means to stably replenish the deteriorating functional b-cell mass detected in both Type 1 and Type 2 Diabetes Mellitus (T1DM and T2DM). Herein we report that over expression of the islet enriched transcription factor Pax4 refrains development of hyperglycemia in the RIP-B7.1 mouse model of T1DM through reduced insulitis, decreased b-cell apoptosis correlating with diminished DNA damage and increased proliferation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Pancreatic beta-cell dysfunction and death are central in the pathogenesis of type 2 diabetes. Saturated fatty acids cause beta-cell failure and contribute to diabetes development in genetically predisposed individuals. Here we used RNA-sequencing to map transcripts expressed in five palmitate-treated human islet preparations, observing 1,325 modified genes. Palmitate induced fatty acid metabolism and endoplasmic reticulum (ER) stress. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Third-party reanalysis

Platform:

GPL9115

10 Samples

Download data: GTF

(Submitter supplied) We single cell sequenced multiplexed primary human islets treated ex vivo with ER and inflammatory chemicals, indexed cell type specific stress responses, found genes to regulate in SC-islets to reduce apoptosis. We conducted multiomic ATAC+RNA sequencing on human islets treated with BFA, DMSO, IL1B+IFNG+TNFa, or PBS

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

13 Samples

Download data: BED, CSV, H5, MTX, TBI, TSV

(Submitter supplied) Pancreatic β-cell failure is key to type 2 diabetes (T2D) onset and progression. We assessed whether human β-cell dysfunction induced by metabolic stress is reversible, evaluated the molecular pathways underlying persistent or transient damage, and explored the relationships with T2D islet traits. Twenty-six human islet preparations were exposed to several lipo- and/or glucotoxicity conditions, some of which impaired insulin release depending on stressor type, concentration and combination. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL9115 GPL16791

131 Samples

Download data: CSV, TAB, TSV