GEO DataSets

(Submitter supplied) Exposure to air particulate matter is associated with increased cardiovascular morbidity and mortality. One of the possible mechanisms is by promotion of atherosclerosis mediated by synergy of particulate matter chemicals and oxidized phospholipids on vascular endothelial cells. We treated human microvascular endothelial cells (HMEC) in triplicate wells with diesel exhaust particles (DEP) extract at a concentration of 5 and 25 mcg/ml, oxidized 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine (ox-PAPC) at concentrations of 10, 20 and 40 mcg/ml or combination of DEP at 5 mcg/ml plus ox-PAPC at 10, 20 and 40 mcg/ml. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2889

Platform:

GPL2700

27 Samples

Download data: TXT

Analysis of microvascular endothelial cells treated at various concentrations of the model air pollutant diesel exhaust particles (DEP), an oxidized phospholipid (ox-PAPC), or both. Results provide insight into the synergistic effect of DEP and ox-PAPC in promoting vascular inflammation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent, 9 dose sets

Platform:

GPL2700

Series:

GSE6584

27 Samples

Download data: TXT

(Submitter supplied) Oxidized phospoholipids are a pro-inflammatory component of minimally modified lipoproteins that get trapped in the subendothelial space of atherosclerotic plaques of large arteries. To model the response of endothelial cells in a pro-atherosclerotic enviroment we measured the expression in primary endothelial cells with and without treatment with oxidized phsopolipids from 96 genetically identical donors of anonymous origin. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3921

629 Samples

Download data: CDF, CEL

(Submitter supplied) Identify genes involved in regulation of inflammatory responses and gene-environemnt interactions, in macrophages from a set of mouse inbred strains termed the HMDP. The HMDP is a genetically diverse mapping panel comprised of classical inbred and recombinant inbred wild type mice. The RMA values of genes were used for genome wide association as described in Bennett et al Genome Research 2010.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8759

511 Samples

Download data: CEL

(Submitter supplied) By means of Operon V3 microarrrays, the in vivo, aortic endothelial transcriptomic responses to chronic (30 day) whole body exposure to diesel exhaust were assessed in wild type and Apo E (-/-) mice. The in vitro response of cultured Svec 4-10 cells exposed to a soluble extract extract of diesel engine particulate were similarly assessed.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7450

10 Samples

Download data: GPR

(Submitter supplied) Air pollution, especially emissions derived from traffic sources, is associated with adverse cardiovascular outcomes. However, it remains unclear how inhaled factors drive an extrapulmonary pathology, as the lung is an effective barrier for solid particulates and many gases. Previously, using plasma from healthy human subjects exposed to diesel exhaust under controlled conditions, we found that canonical inflammatory response transcripts of interleukin-8 (IL-8), intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were elevated in endothelial cells treated wih plasma obtained after exposure compared with pre-exposure samples or filtered air (sham) exposures. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

48 Samples

Download data: IDAT, TXT

(Submitter supplied) The potential of diesel exhaust particles (DEP) to transform human bronchial epithelial cells (HBEC3) was investigated and a stably transformed cell line (T2-HBEC3) was established. Short-term DEP exposure experiments adds information of immunomodulatory effect markers and differences in susceptibility between normal and sensitized bronhial epithelial cells of the human lung.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

42 Samples

Download data: TXT

(Submitter supplied) Rationale: Maternal immune responses can promote allergy development in offspring. Pilot data show that neonates of mother mice exposed during pregnancy to air pollution particles have increased allergic susceptibility. Objective: We investigated whether inflammatory response to titanium dioxide (TiO2) particles earlier considered immunologically ‘inert’ is enhanced during pregnancy. Methods: Pregnant BALB/c mice (or non-pregnant controls) received particle suspensions intranasally at day 14 of pregnancy. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2878

Platform:

GPL1261

16 Samples

Download data: CEL, CHP

Analysis of lungs from pregnant and non-pregnant BALB/c females following insufflation with inert titanium dioxide particles. Results provide insight into the mechanisms underlying the differences in response between pregnant and non-pregnant females to inhaled inert particles.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 development stage sets

Platform:

GPL1261

Series:

GSE7475

16 Samples

Download data: CEL, CHP

(Submitter supplied) Epidemiology studies have linked exposure to pollutant particles to increased cardiovascular mortality and morbidity, however, the mechanism remains unknown. In this study, we hypothesized that the ultrafine fraction of ambient pollutant particles would cause endothelial cells dysfunction. We profiled gene expression of human pulmonary artery endothelial cells (HPAEC) exposed to ultrafine Chapel Hill particles (UFP) (100μg/ml) or vehicle for 4h with Affymetrix HG U133 Plus 2.0 chips (N = 4 each). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2565

Platform:

GPL570

8 Samples

Download data: CEL

Analysis of pulmonary artery endothelial cells HPAEC exposed to ultrafine particles for 4 hours. Results provide insight into the molecular basis of the association of pollutant particle exposure to increased cardiovascular mortality and morbidity.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL570

Series:

GSE4567

8 Samples

Download data: CEL

(Submitter supplied) While much progress has been made in identifying the mechanisms that trigger endothelial activation and inflammatory cell recruitment during atherosclerosis, less is known about the intrinsic pathways that counteract these events. Here we identified NOTCH1 as an antagonist of endothelial cell activation. NOTCH1 was constitutively expressed by adult arterial endothelium, but levels were significantly reduced by high fat diet. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

9 Samples

Download data: IDAT