Similar studies for GEO DataSets (Select 200013146) - GEO DataSets

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Items: 20

Select item 2000131461.

Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts

(Submitter supplied) Induced pluripotent stem (iPS) cells can be generated from somatic cells by transduction with several transcription factors in both mouse and human. However, direct reprogramming in other species has not been reported. Here, we established an efficient method to generate monkey iPS cells from fibroblasts by retrovirus-mediated introduction of the four monkey transcription factors OCT4 (POU5F1), SOX2, KLF4, and c-MYC. more...

Organism:: Macaca mulatta

Type:: Expression profiling by array

Platform:: GPL3535
9 Samples

Download data: CEL, XLS

Series

Accession:: GSE13146

ID:: 200013146

Select item 2000108062.

Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors

(Submitter supplied) Reprogramming of somatic cells is a valuable tool to understand the mechanisms of regaining pluripotency and further opens up the possibility of generating patient-specific pluripotent stem cells. Reprogramming of mouse and human somatic cells into pluripotent stem cells, designated as induced pluripotent stem (iPS) cells, has been possible with the expression of the transcription factor quartet Oct4 (also known as Pou5f1), Sox2, c-Myc, and Klf4. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
11 Samples

Download data: CEL

Series

Accession:: GSE10806

ID:: 200010806

Select item 2000125833.

Highly efficient generation of induced pluripotent stem cells from human keratinocytes

(Submitter supplied) The generation of induced pluripotent stem (iPS) cells 1-4 has spawned unprecedented opportunities for investigating the molecular logic that underlies cellular pluripotency and reprogramming, as well as for obtaining patient-specific cells for future clinical applications. However, both prospects are hampered by the low efficiency of the reprogramming process. Here, we show that juvenile human primary keratinocytes can be efficiently reprogrammed to pluripotency by retroviral transduction with Oct4, Sox2, Klf4 and c-Myc. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
9 Samples

Download data: CEL

Series

Accession:: GSE12583

ID:: 200012583

Select item 2000097094.

Human induced pluripotent stem (iPS) cells from neonatal skin derived cells

(Submitter supplied) Induction of germline-competent pluripotent stem cells from mouse fibroblasts has been achieved by the ectopic expression of four genes (Oct3/4, Sox2, c-Myc and Klf4). If this method can be applied to humans for the generation of personalized human pluripotent stem cells, it would greatly facilitate the therapeutic application of stem cells by avoiding the problem of immune rejection by the recipient associated with allograft transplants. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platforms:: GPL1708 GPL570
13 Samples

Download data: CEL, TXT

Series

Accession:: GSE9709

ID:: 200009709

Select item 2000095615.

human induced pluripotent stem cell

(Submitter supplied) Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL4133
2 Samples

Download data: TXT

Series

Accession:: GSE9561

ID:: 200009561

Select item 2000215166.

Direct Reprogramming of Fibroblasts into Epiblast Stem Cells

(Submitter supplied) Embryonic stem cells (ESCs), which are derived from the primitive ectoderm of pre-implantation blastocysts, are pluripotent cells and can thus contribute to the formation of all somatic cell lineages in chimeric animals. Similarly, epiblast stem cells (EpiSCs), which are derived from epiblast tissue of post-implantation embryos, are also pluripotent and can give rise to derivatives of all three germ layers in teratoma assays. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6885
24 Samples

Download data: TXT

Series

Accession:: GSE21516

ID:: 200021516

Select item 2000281977.

Expression analysis of stepwise induction of iPS cells

(Submitter supplied) We were able to achieve an initial stable intermediate phase by the transduction of Oct4, Klf4, and c-Myc. Furthermore, over-expression of Sox2 in these intermediate stage cells leads to final iPS cell phase. After examining the gene expression profiles from the initial to final iPS cell phases, we have identified Sox2 downstream genes important for iPS cell induction.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6885
16 Samples

Download data: TXT

Series

Accession:: GSE28197

ID:: 200028197

Select item 2000098328.

Reprogramming of human somatic cells to pluripotency with defined factors

(Submitter supplied) Pluripotency, the capacity of embryo-derived stem cells to generate all tissues in the organism, can be induced in somatic cells by nuclear transfer into oocyte, fusion with embryonic stem cells, and for male germ cells by cell culture alone. Recently, murine fibroblasts have been reprogrammed directly to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4, and Myc) to yield induced Pluripotent Stem (iPS) cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
16 Samples

Download data: CEL, CHP

Series

Accession:: GSE9832

ID:: 200009832

Select item 2000132129.

Global gene-expression analyses of the Esrrb knockdown cells

(Submitter supplied) In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to convert fibroblasts to induced pluripotent stem (iPS) cells that exhibit many characteristics of embryonic stem (ES) cells. Herein, we show that the orphan nuclear receptor Esrrb works in conjunction with Oct4 and Sox2 to mediate reprogramming of mouse embryonic fibroblasts (MEFs) to iPS cells. Esrrb reprogrammed cells share similar expression and epigenetic signatures as ES cells. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6105
18 Samples

Download data: TXT

Series

Accession:: GSE13212

ID:: 200013212

Select item 20001321110.

Global gene-expression analyses of the Esrrb reprogrammed cells

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6105
16 Samples

Download data: TXT

Series

Accession:: GSE13211

ID:: 200013211

Select item 20001319011.

Global gene-expression analyses of the Esrrb reprogrammed cells and Esrrb knockdown cells

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6105
34 Samples

Download data

Series

Accession:: GSE13190

ID:: 200013190

Select item 20006606712.

Genome-wide map of SET1A occupancy in mouse ES cells

(Submitter supplied) To identify SET1A genome-wide occupancy and further unveil its role in transcriptional regulation in mouse ES cells, we carried out chromatin immunoprecipitation followed by high sequencing (ChIP-seq).We established a stable ES cell line expressing 2X Flag tagged SET1A and performed ChIP with anti-Flag M2 beads, followed by deep sequencing. We found that the SET1A peaks show an outstanding enrichment in promoter region. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL13112
2 Samples

Download data: BED, BW

Series

Accession:: GSE66067

ID:: 200066067

PubMed Similar studies SRA Run Selector

Select item 20002868813.

Molecular insights into induced pluripotency mediated by the OCT4, SOX2, KLF and c-MYC gene regulatory network

(Submitter supplied) Human somatic fibroblasts can be reprogrammed to induced pluripotent stem (iPS) cells by exogenic expression of the Yamanaka factors (OCT4, SOX2, KLF4 and MYC) after about 1 month. To gain some insight into the early processes operative in fibroblast reprogramming, we profiled genome-wide transcription levels using Illumina microarrays in the starting donor cells-human foreskin fibroblast (HFF1) cells and at three time points after OSKM transduction (24 h, 48 h, 72 h), as well as two iPS cell lines (iPS2, iPS4) and hES cell lines (H1, H9). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6883
14 Samples

Download data: TXT

Series

Accession:: GSE28688

ID:: 200028688

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003784214.

Generation and maintenance of hiPSCs on PCM-DM

(Submitter supplied) Human ES cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are usually generated and maintained on living feeder cells like mouse embryonic fibroblasts or on a cell-free substrate like Matrigel. For clinical applications, a quality-controlled, xenobiotic-free culture system is required to minimize risks from contaminating animal-derived pathogens and immunogens. We previously reported that the pericellular matrix of decidua-derived mesenchymal cells (PCM-DM) is an ideal human-derived substrate on which to maintain hiPSCs/hESCs. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
4 Samples

Download data: CEL

Series

Accession:: GSE37842

ID:: 200037842

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003628915.

Comparison of the gene expression profiles between MEF, MEF treated with n-Butylenephthalide (BP) 10 and 40ug/ml.

(Submitter supplied) The microarray analysis was used for comparing the gene expression profiles between MEF, MEF treated with n-Butylenephthalide (BP) 10 and 40ug/ml.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
3 Samples

Download data: TXT

Series

Accession:: GSE36289

ID:: 200036289

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006256516.

Efficient differentiation of steroidogenic and germ-like cells from epigenetically-related iPSCs derived from ovarian granulosa cells

(Submitter supplied) To explore restoration of ovarian function using epigenetically-related, induced pluripotent stem cells (iPSCs), we functionally evaluated the epigenetic memory of novel iPSC lines, derived from mouse and human ovarian granulosa cells (GCs) using c-Myc, Klf4, Sox2 and Oct4 retroviral vectors. The stem cell identity of the mouse and human GC-derived iPSCs (mGriPSCs, hGriPSCs) was verified by demonstrating embryonic stem cell (ESC) antigen expression using immunocytochemistry and RT-PCR analysis, as well as formation of embryoid bodies (EBs) and teratomas that are capable of differentiating into cells from all three germ layers. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6887
18 Samples

Download data: TXT

Series

Accession:: GSE62565

ID:: 200062565

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20001535517.

Direct Reprogramming of Human Neural Stem Cells by OCT4

(Submitter supplied) Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of the four transcription factors (OCT4, SOX2, c-MYC, KLF4). We previously reported that Oct4 alone is sufficient to directly reprogram adult mouse neural stem cells (NSCs) to iPS cells. Here, we report the generation of one-factor (1F) human iPS from human NSCs (1F hNiPS) by ectopic expression of Oct4 alone. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6883
10 Samples

Download data: TXT

Series

Accession:: GSE15355

ID:: 200015355

Select item 20011037718.

RNA-seq profiling and DNA methylation of human somatic, primed and naïve induced Pluripotent Stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:: GPL18573
23 Samples

Download data: BEDGRAPH, TXT

Series

Accession:: GSE110377

ID:: 200110377

PubMed Full text in PMC Similar studies

Select item 20011036619.

Profiling the DNA methylation pattern in naïve induced Pluripotent Stem cells and somatic cells

(Submitter supplied) We analysed by Reduced Representation Bisulfite Sequencing (RRBS) the genome methylation pattern in human fibroblast and in naïve induced pluripoent stem cells (niPSCs)