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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 15, 2009 |
| Title |
Global gene-expression analyses of the Esrrb knockdown cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to convert fibroblasts to induced pluripotent stem (iPS) cells that exhibit many characteristics of embryonic stem (ES) cells. Herein, we show that the orphan nuclear receptor Esrrb works in conjunction with Oct4 and Sox2 to mediate reprogramming of mouse embryonic fibroblasts (MEFs) to iPS cells. Esrrb reprogrammed cells share similar expression and epigenetic signatures as ES cells. These cells are also pluripotent and can differentiate in vitro and in vivo into the three major embryonic cell lineages. Furthermore, these cells contribute to mouse chimeras and are germline transmissible. In ES cells, Esrrb targets many genes involved in selfrenewal and pluripotency. This suggests that Esrrb may mediate reprogramming through the up-regulation of ES cell-specific genes. Our findings also indicate that it is possible to reprogram MEFs without exogenous Klf transcription factors and link a nuclear receptor to somatic cell reprogramming.
Global gene expression effects of silencing the Esrrb gene. We used microarrays to detail the global programme of gene expression after silencing the Esrrb gene.
Keywords: time-course
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| Overall design |
Three biological replicates each for control GFP and Esrrb RNAi. The global gene expression profiles of the Esrrb knockdown cells were compared to control GFP knockdown cells for days 2, 4 and 6.
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| Contributor(s) |
Feng B, Jiang J, Kraus P, Ng J, Heng J, Chan Y, Yaw L, Zhang W, Loh Y, Han J, Vega VB, Cacheux Rataboul V, Lim B, Lufkin T, Ng H |
| Citation(s) |
19136965 |
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| Submission date |
Oct 15, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Vinsensius B Vega |
| E-mail(s) |
[email protected]
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| Organization name |
Genome Institute of Singapore
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| Street address |
60 Biopolis Street
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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| Samples (18)
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| GSM333653 |
Control GFP RNAi, day 2, biological replicate 1 |
| GSM333654 |
Control GFP RNAi, day 2, biological replicate 2 |
| GSM333655 |
Control GFP RNAi, day 2, biological replicate 3 |
| GSM333656 |
Esrrb RNAi, day 2, biological replicate 1 |
| GSM333657 |
Esrrb RNAi, day 2, biological replicate 2 |
| GSM333658 |
Esrrb RNAi, day 2, biological replicate 3 |
| GSM333659 |
Control GFP RNAi, day 4, biological replicate 1 |
| GSM333660 |
Control GFP RNAi, day 4, biological replicate 2 |
| GSM333661 |
Control GFP RNAi, day 4, biological replicate 3 |
| GSM333662 |
Esrrb RNAi, day 4, biological replicate 1 |
| GSM333663 |
Esrrb RNAi, day 4, biological replicate 2 |
| GSM333664 |
Esrrb RNAi, day 4, biological replicate 3 |
| GSM333665 |
Control GFP RNAi, day 6, biological replicate 1 |
| GSM333666 |
Control GFP RNAi, day 6, biological replicate 2 |
| GSM333667 |
Control GFP RNAi, day 6, biological replicate 3 |
| GSM333668 |
Esrrb RNAi, day 6, biological replicate 1 |
| GSM333669 |
Esrrb RNAi, day 6, biological replicate 2 |
| GSM333670 |
Esrrb RNAi, day 6, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE13190 |
Global gene-expression analyses of the Esrrb reprogrammed cells and Esrrb knockdown cells |
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| Relations |
| BioProject |
PRJNA114037 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13212_Esrrb_RNAi_RawData.txt |
30.5 Mb |
(ftp)(http) |
TXT |
| GSE13212_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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