GEO DataSets

(Submitter supplied) Establishment of an in vitro system to explore molecular mechanisms of mastitis susceptibility in cattle by comparative expression profiling of Escherichia coli and Staphylococcus aureus inoculated primary cells sampled from cows with different genetic predisposition for somatic cell score Primary bovine mammary gland epithelial cells (pbMEC) were sampled from the udder parenchyma of cows that were selected for high and low mastitis susceptibility by applying a marker assisted selection strategy considering QTL and molecular marker information of a repetitively confirmed QTL for SCS in the telomeric region on BTA18 The cells were cultivated and subsequently inoculated with heat inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4437

Platform:

GPL2112

88 Samples

Download data: CEL

Mammary gland epithelial cells of cows with favorable somatic cell score (SCS) QTL allele Q and unfavorable allele q after S. aureus or E.coli exposure for up to 24 hours. SCS is an indicator of milk quality and udder health. Results provide insight into molecular basis of mastitis susceptibility.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL2112

Series:

GSE24560

88 Samples

Download data: CEL

(Submitter supplied) Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Mastitis caused by Escherichia coli and Staphylococcus aureus is a major pathology of dairy cows. To better understand the differential response of the mammary gland to these two pathogens, we stimulated bovine mammary epithelial cells (bMEC) with either E. coli crude lipopolysaccharide (LPS) or with S. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL17231

32 Samples

Download data: TXT

(Submitter supplied) Outcome and resolution of an udder inflammation (mastitis) depends on the type of the invading pathogen. Gram-negative pathogens, such as Escherichia coli mostly trigger strong inflammation and full local activation of the immune defense eventually resulting in pathogen elimination. Staphylococcus aureus and other Gram-positive pathogens elicit only weak immune reactions possibly allowing for pathogen persistence. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL17645

49 Samples

Download data: CEL

(Submitter supplied) Mastitis in dairy cows is one of the most costly and prevalent diseases affecting dairy cows world wide. Insight in the molecular regulation of the host immune response to an E. coli infection, could help to develop new strategies to prevent cattle from E. coli infection. Here we performed a gene-expression analysis from udder tissue exposed to a controlled E. coli infection at T=24h post infection (p.i.) representing the acute phase response and at T=192h p.i. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

49 Samples

Download data: CEL

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

36 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to explore the lncRNA profile on mastitis.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

9 Samples

Download data: XLSX

(Submitter supplied) Mastitis is a common disease in dairy cows and brings massive losses to the dairy industry. m6A is a type of modification strongly associated with many diseases. However, the role of m6A in mastitis caused by Staphylococcus aureus and Escherichia coli has not been investigated.We used MeRIP-seq technology to sequence the bovine mammary epithelial cells (MAC-T) infected with inactivated S. aureus/E. more...

Organism:

Bos taurus

Type:

Other

Platform:

GPL23295

18 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

30 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of udder quarters neighboring to infected quarters and healthy udder tissue we analyse gene expression in the late stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define systemic reactions to infection with E. coli 1303. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of infected and healthy udder tissue we analyse gene expression in the late stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define late stage of infection with E. coli 1303.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of infected and healthy udder tissue we analyse gene expression in the early stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define early stage of infection with E. coli 1303. Keywords: disease state analysis

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) Purpose: The aim of the study was to compare the miRNA expression in non-infected (H) mammary gland parenchyma samples with that of glands infected with coagulase-positive staphy lococci (CoPS) or coagulase-negative staphylococci (CoNS) using next-generation sequencing. Methods: miRNA-seq analysis was performed on mammary gland parenchyma samples collected from non-infected cows and those infected with coagulase-positive or -negative staphylococci. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17875

36 Samples

Download data: FA

(Submitter supplied) The regulatory effects of H3K27me3 on target genes expressions were analyzed by comparing S. aureus mastitis resistant and susceptible cows. The differentially expressed genes are mainly associated with immune and disease-related processes, which were negatively regulated by H3K27me3 modification on the up 2Kb regions relative to TSS in S. aureus mastitis cattle.

Organism:

Bos taurus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15749

6 Samples

Download data: TAR

(Submitter supplied) Purpose: The goal of this study is to explore the role of miRNAs in dairy cow response to E. coli and S. aureus, mastitis causing pathogens, is not well understood. Results: The global expression of miRNAs in bovine mammary epithelial cells (MAC-T cells) challenged with heat-inactivated Staphylococcus aureus (S. aureus) or Escherichia coli (E. coli) bacteria (treatments: 6, 12, 24 and 48 hr) and without challenge (control: 0, 6, 12, 24 and 48 hr) was profiled using next-generation-sequencing. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17875

13 Samples

Download data: TXT

(Submitter supplied) Infections of the udder by Escherichia coli very often elicit acute inflammation, while Staphylococcus aureus infections tend to cause mild, subclinical inflammation and persistent infections. The molecular causes undercovering the different disease patterns are poorly understood. We therefore profiled kinetics and extent of global changes in the transcriptome of primary bovine mammary epithelia cells (MEC) subsequent to challenging them with heat inactivated preparations of E. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4406

Platform:

GPL2112

30 Samples

Download data: CEL

Analysis of mammary epithelial cells (MECs) challenged with heat-killed particles of E. coli or S. aureus for up to 24 hrs. Results provide insight into the molecular mechanisms underlying the pathogen-specific disease patterns.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 4 stress, 5 time sets

Platform:

GPL2112

Series:

GSE25413

30 Samples

Download data: CEL