GEO DataSets

(Submitter supplied) The response regulator HrrA belonging to the HrrSA two-component system (previously named CgtSR11) is known to be repressed by the global iron-dependent regulator DtxR in Corynebacterium glutamicum. Sequence analysis indicated an involvement of the HrrSA system in heme-dependent gene expression. Growth experiments revealed that the non-pathogenic soil bacterium C. glutamicum is able to use hemin or hemoglobin as sole iron source. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

9 Samples

Download data: GPR

(Submitter supplied) We recently showed that the two-component system (TCS) HrrSA plays a central role in the control of heme homeostasis in the Gram-positive soil bacterium Corynebacterium glutamicum. Here, we characterized the function of another TCS of this organism, ChrSA, which exhibits significant sequence similarity to HrrSA, and provide evidence for cross-regulation of the two systems. In this study ChrSA was shown to be crucial for heme resistance of C. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL15451

6 Samples

Download data: GPR

(Submitter supplied) We evaluated how HrrA binding in response to 4 µM heme as initial stimulus. Analysis was performed prior to the addition of heme (T0) and 0.5, 2, 4, 9, and 24 h after the heme pulse (in medium containing no other iron source).

Organism:

Corynebacterium glutamicum

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25746

6 Samples

Download data: BED

(Submitter supplied) We evaluated how HrrA binding (found by ChAP-Seq) impacts the expression of individual target genes, by analyzing the transcriptome of the C. glutamicum wild type strain (ATCC 13032) as well as a ∆hrrA mutant. RNA-Seq analysis was performed prior to the addition of heme (T0) and 0.5 and 4 h after the heme pulse (in medium containing no other iron source).

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25416

6 Samples

Download data: TSV

(Submitter supplied) RNA-seq differential gene expression profiling of Corynebacterium pseudotuberculosis under iron limitation. We use two C. pseudotuberculosis strains, a ciuA Cp13 mutant and its parental wild type T1 strain to quantify the relative gene expression of these strains in cultures with low iron availability. The ciuA Cp13 mutant is deficient for an operon which encodes a iron siderophore uptake system. The Cp13 mutant also showed reduced virulence in CLA infection models. more...

Organism:

Corynebacterium pseudotuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24971

14 Samples

Download data: TXT

(Submitter supplied) Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu2+ was studied using DNA microarrays and revealed 26 genes that showed a ≥3-fold increased mRNA level, including cg3280-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

9 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum R

Type:

Expression profiling by array; Genome binding/occupancy profiling by array

Platforms:

GPL17881 GPL20864 GPL20865

14 Samples

Download data: TXT

(Submitter supplied) Bacteria modify expression of different types of terminal oxidase in response to oxygen availability. Corynebacterium glutamicum, a facultative anaerobic bacterium in Actinobacteria, possesses aa3-type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced upon oxygen limitation. We report here that an extracytoplasmic function sigma factor, SigC, is unprecedentedly responsible for the regulation. more...

Organism:

Corynebacterium glutamicum R

Type:

Genome binding/occupancy profiling by array

Platform:

GPL20865

4 Samples

Download data: TXT

(Submitter supplied) Bacteria modify expression of different types of terminal oxidase in response to oxygen availability. Corynebacterium glutamicum, a facultative anaerobic bacterium in Actinobacteria, possesses aa3-type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced upon oxygen limitation. We report here that an extracytoplasmic function sigma factor, SigC, is unprecedentedly responsible for the regulation. more...

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum R

Type:

Expression profiling by array

Platform:

GPL20864

4 Samples

Download data: TXT

(Submitter supplied) Bacteria modify expression of different types of terminal oxidase in response to oxygen availability. Corynebacterium glutamicum, a facultative anaerobic bacterium in Actinobacteria, possesses aa3-type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced upon oxygen limitation. We report here that an extracytoplasmic function sigma factor, SigC, is unprecedentedly responsible for the regulation. more...

Organism:

Corynebacterium glutamicum R

Type:

Expression profiling by array

Platform:

GPL17881

6 Samples

Download data: TXT

(Submitter supplied) Corynebacterium glutamicum, a gram-positive soil bacterium used for the industrial production of amino acids such as L-glutamate and L-lysine, is able to use a number of different nitrogen sources, such as ammonium, urea, or creatinine. In this communication, we show that L-glutamine serves as an excellent nitrogen source for C. glutamicum and allows similar growth rates in glucose minimal medium as ammonium. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

3 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of Corynebacterium glutamicum cells comparing wild-type cells with cg0196 deletion mutant cells by site-specific gene deletion using the non-replicable integration vector. cg0196 is gene conding transcriptional regulator related carbon metabolism.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL14656

1 Sample

Download data: GPR

(Submitter supplied) To identify genes which are differentially expressed in Corynebacterium glutamicum in the cg2699 deletion strain, we performed DNA microarray analyses of C. glutamicum Δcg2699 compared to the WT.

Organism:

Gluconobacter oxydans; Corynebacterium glutamicum ATCC 13032; Bacillus subtilis subsp. subtilis str. 168; Escherichia coli; Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL16989

3 Samples

Download data: GPR

(Submitter supplied) To identify genes which are differentially expressed in Corynebacterium glutamicum in the absence of copper, we performed DNA microarray analyses of cells cultivated under copper starvation conditions compared to copper sufficiency.

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL15451

3 Samples

Download data: GPR

(Submitter supplied) DNA affinity chromatography with the promoter region of the Corynebacterium glutamicum pck gene, encoding phosphoenolpyruvate carboxykinase (PEPCk), led to the isolation of four transcriptional regulators, i.e., RamA, GntR1, GntR2 and IolR. Determination of the PEPCk activity of the deletion mutants ΔramA, ΔgntR1ΔgntR2, and ΔiolR indicated that RamA represses pck during growth on glucose about twofold, whereas GntR1, GntR2, and IolR activate pck expression about twofold, irrespective whether glucose or acetate served as carbon source. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL15451

3 Samples

Download data: GPR

(Submitter supplied) <Background> The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the P i starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ∆phoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL19905

2 Samples

Download data: CSV

(Submitter supplied) Corynebacterium glutamicum GlxR is a homolog of the cAMP receptor protein. Although over 200 GlxR binding sites in the C. glutamicum genome are predicted in silico, studies on the GlxR physiological function have been hindered by the severe growth defects of a glxR mutant. This study comprehensively identified the GlxR regulon by chromatin immunoprecipitation in conjunction with microarray (ChIP-chip) analyses. more...

Organism:

Corynebacterium glutamicum

Type:

Genome binding/occupancy profiling by array

Platform:

GPL11651

7 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pseudomonas putida; Escherichia coli; Corynebacterium glutamicum; Gluconobacter oxydans; Corynebacterium glutamicum ATCC 13032; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platforms:

GPL22794 GPL22792

6 Samples

Download data: GPR

(Submitter supplied) Iron is the fourth most abundant element in the Earth’s crust. However, the poor solubility of iron due to oxidation of ferrous iron to the almost insoluble ferric iron under aerobic conditions constitutes a considerable challenge for living organisms to obtain sufficient amounts of the iron available. In the present study, we set out to characterize the global gene expression of C. glutamicum under iron limitation in comparison to iron-replete conditions.

Organism:

Escherichia coli; Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Bacillus subtilis subsp. subtilis str. 168; Pseudomonas putida; Gluconobacter oxydans

Type:

Expression profiling by array

Platform:

GPL22794

3 Samples

Download data: GPR

(Submitter supplied) Iron is the fourth most abundant element in the Earth’s crust. However, the poor solubility of iron due to oxidation of ferrous iron to the almost insoluble ferric iron under aerobic conditions constitutes a considerable challenge for living organisms to obtain sufficient amounts of the iron available. In the present study, we set out to characterize the global gene expression of C. glutamicum under iron limitation in comparison to iron-replete conditions.

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Escherichia coli; Gluconobacter oxydans; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL22792

3 Samples

Download data: GPR