GEO DataSets

(Submitter supplied) In the oral biofilm, the mitis streptococci are among the first group of organisms to colonize the tooth surface. Their proliferation is thought to be an important factor required for antagonizing the growth of cariogenic species such as Streptococcus mutans. In this study, we used a 3-species mixed culture to demonstrate that another ubiquitous early colonizing species, Veillonella parvula, could greatly impact the competitive outcome of a mixed culture of S. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

8 Samples

Download data: CEL

(Submitter supplied) To investigate the inhibition mechanism of copper ions on S. mutans-V. parvula dual biofilm.

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28465

4 Samples

Download data: TXT

(Submitter supplied) RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. more...

Organism:

Streptococcus gordonii; Streptococcus sobrinus; Lacticaseibacillus casei; Streptococcus mutans UA159; Streptococcus oralis; Corynebacterium matruchotii; Streptococcus sanguinis; Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

11 related Platforms

33 Samples

Download data: CSV, XLSX

(Submitter supplied) Investigation of whole genome gene expression levels of P. gingivalis W83, F. nucleatum DSMZ 25586, S. sanguinis SK36, A. actinomycetemcomitans HK1651, S. mutans UA159 in an 24 h old culture. Additionally, whole genome gene expression level changes of S. mutans UA159 biofilm cells after co-cultivation with S. mitis ATCC 11843 were compared to its single species biofilm growth after 24 h. Aim: Demonstration of the usefulness of a five-species gene expression array. more...

Organism:

Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans HK1651; Streptococcus mutans UA159; Porphyromonas gingivalis W83; Streptococcus mitis; Streptococcus sanguinis SK36

Type:

Expression profiling by array

Platforms:

GPL13444 GPL10873

12 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Streptococcus mutans, the organism most frequently associated with the development of dental caries, is able to utilize a diverse array of carbohydrates for energy metabolism. One such molecule is trehalose, a disaccharide common in human foods, which has recently been implicated in enhancing the virulence of epidemic strains of the pathogen, Clostridium difficile. In this study, deletion mutants of all three genes in the putative S. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24591

18 Samples

Download data: TXT, XLSX

(Submitter supplied) Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either up- or down-regulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were statistically (P ≤0.05) upregulated, while 36 genes mainly involved in transport and translation were down-regulated. more...

Organism:

Streptococcus gordonii; Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20135 GPL19036 GPL20134

15 Samples

Download data: TXT

(Submitter supplied) We performed comparative analysis of transcriptomes of S. mutans in single biofilms and in mixed-biofilms with A. actinomycetemcomitans. We also compared the transcriptomic profiles of A. actinomycetemcomitans in single biofilms and A. actinomycetemcomitans in mixed biofilms with S. mutans. Finally we looked at the changes in gene expression in both organisms in time.

Organism:

Aggregatibacter actinomycetemcomitans; Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21141 GPL21140 GPL21142

20 Samples

Download data: XLSX

(Submitter supplied) Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 minutes to assess the streptococcal transcriptome response. more...

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL20180

2 Samples

Download data: TXT

(Submitter supplied) Oral streptococci, including Streptococcus gordonii, and Actinomyces naeslundii, are consistently found to be the most abundant bacteria in the early stages of dental plaque accumulation. These organisms interact physically (coaggregate) in vitro and in vivo. We hypothesized that coaggregation between S. gordonii and A. naeslundii leads to changes in gene expression in the partner organisms. Furthermore, we predicted that coaggregation-induced changes in phenotype contribute to the success of streptococci and actinomyces in dental plaque. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL5736

3 Samples

Download data: MEV

(Submitter supplied) Using DNA-microarrays analysis 39 differentially expressed genes were identified in 200 vs. 100 microns biofilms, which might be associated with thickness of S. mutans biofilm. Using DNA-microarrays analysis 29 differentially expressed genes were identified in 400 vs. 100 microns biofilms of S. mutans.

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4745

5 Samples

Download data: GPR

(Submitter supplied) We assesed the transcriptional response of wild-type and isogenic lrgAB mutant to growth in the presence of three different stress conditions (aerobiosis, high-temperature, and the cell-wall active antibiotic vancomycin) by using RNA-seq

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

24 Samples

Download data: TXT

(Submitter supplied) Our group recently transcriptomically characterized coculture growth between Streptococcus mutans and several species of commensal streptococci (Rose et al, 2023). However, these experiments were carried out in our lab-based experimental medium, tryptone and yeast extract (TY-). To understand whether culturing these species within a medium that more closely mimics their natural environment alters the interaction, we evaluated both monoculture and coculture growth between the dental caries pathogen Streptococcus mutans and oral commensal species Streptococcus oralis in a half TY- / half human saliva mix that was optimally chosen based on our initial characterization of oral streptococci behaviors in medium mixes containing saliva. more...

Organism:

Streptococcus oralis; Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33629 GPL33630 GPL24591

18 Samples

Download data: CSV