GEO DataSets

(Submitter supplied) We used microarrays to assess gene expression profiling of 6 patients with a mutation (Arg1174Gln) in the tyrosine kinase domain of the insulin receptor gene (INSR) and 10 matched healthy controls

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4897

Platform:

GPL571

16 Samples

Download data: CEL

Analysis of muscle from patients with a mutation (Arg1174Gln) in the tyrosine kinase domain of the insulin receptor gene (INSR). This mutation is associated with inherited insulin resistance. Results provide insight into molecular mechanisms underlying insulin resistance in skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL571

Series:

GSE36297

16 Samples

Download data: CEL

(Submitter supplied) To gain a mechanistic insight into TET3-mediated regulation of muscle insulin sensitivity, we performed genome-wide expression profiling (RNA-seq) on RNA isolated from GAS muscles of muscle TET3 specific knockdown mice and WT littermates.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: XLSX

(Submitter supplied) Recently, abnormalities in mitochondrial oxidative phosphorylation (OXPHOS) have been implicated in the pathogenesis of skeletal muscle insulin resistance in type 2 diabetes. In the present study, we hypothesized that decreased expression of OXPHOS genes could be of similar importance for insulin resistance in the polycystic ovary syndrome (PCOS). Using the HG-U133 Plus 2.0 expression array from Affymetrix, we analyzed gene expression in skeletal muscle from obese women with PCOS (n=16) and age- and body mass index-matched control women (n=13) metabolically characterized by euglycemic-hyperinsulinemic clamp and indirect calorimetry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3104

Platform:

GPL570

29 Samples

Download data: CEL

Analysis of vastus lateralis muscles from women with insulin-resistant polycystic ovary syndrome (PCOS). Insulin resistance in skeletal muscles is a risk factor for the development of type 2 diabetes in women with PCOS. Results provide insight into the pathogenesis of insulin resistance in PCOS.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL570

Series:

GSE6798

29 Samples

Download data: CEL

(Submitter supplied) Decreased mitochondrial mass and function in muscle of diabetic patients is associated with low PGC-1alpha, a transcriptional coactivator of the mitochondrial gene program. To investigate whether reduced PGC-1alpha and oxidative capacity in muscle directly contributes to age-related glucose intolerance, we compared the genetic signatures and metabolic profiles of aging mice lacking muscle PGC-1alpha. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4904

Platform:

GPL1261

12 Samples

Download data: CEL

Analysis of muscle from aged animals with muscle-specific Pgc-1α depletion. PGC-1alpha is a transcriptional coactivator of the mitochondrial gene program. Results provide insight into the role of Pgc-1α in the glucose intolerance and chronic systemic inflammation associated with aging.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 age, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE52550

12 Samples

Download data: CEL

(Submitter supplied) To identify mediators of obesity-linked reductions in PGC-1, we tested the effects of cellular nutrients in C2C12 myotubes. While overnight exposure to high insulin, glucose, glucosamine, or amino acids had no effect, saturated fatty acids (FA) potently reduced PGC-1a and b mRNA expression. Keywords: Nutrient Effect

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2648

Platform:

GPL8321

6 Samples

Download data: CEL

Analysis of myoblasts treated with the saturated fatty acid palmitate. Muscle expression of PPAR coactivator 1 (PGC-1) is reduced in models of obesity. Palmitate decreases the expression of PGC-1. Results provide insight into the molecular basis of the link between overnutrition, obesity, and PGC-1.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL8321

Series:

GSE6766

6 Samples

Download data: CEL

(Submitter supplied) Transcriptional microarray analysis was conducted on gastrocnemius muscle of control and PGC-1β(i)skm-/- mice one week after the last tamoxifen administration using the Affymetrix Mouse Gene 1.0 ST.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

2 Samples

Download data: CEL, CHP

(Submitter supplied) Context: Exercise training is a plausible model for identification of molecular mechanisms that cause metabolic-related changes in human skeletal muscle. Objective: The goal was to explore the molecular basis of the adaptation of skeletal muscle to exercise training. Design and Intervention: Obese male subjects were subjected to an individualized supervised training program targeted in order to optimize lipid oxidation during 8 weeks. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16022

16 Samples

Download data: GPR

(Submitter supplied) Microarray-based studies of skeletal muscle from patients with type 2 diabetes and high-risk individuals have demonstrated that insulin resistance and reduced mitochondrial biogenesis co-exist early in the pathogenesis of type 2 diabetes independent of hyperglycaemia and obesity. It is unknown whether reduced mitochondrial biogenesis or other transcriptional alterations co-exist with impaired insulin-responsiveness in primary human muscle cells from patients with type 2 diabetes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3681

Platform:

GPL8300

20 Samples

Download data: CEL

Analysis of myotube cell lines established from type 2 diabetes (T2D) subjects. Insulin resistance and reduced mitochondrial biogenesis coexist early in T2D pathogenesis independent of hyperglycemia and obesity. Results provide insight into the effect of T2D on developing skeletal muscle cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL8300

Series:

GSE12643

20 Samples

Download data: CEL

(Submitter supplied) PGC1beta is a transcriptional coactivator that potently stimulates mitochondrial biogenesis and respiration of cells. Here, we have generated mice lacking exons 3 to 4 of the Pgc1beta gene (PGC1beta E3,4-/E3,4- mice). These mice express a mutant protein that has reduced coactivation activity on a subset of transcription factors, including ERRalpha, a major target of PGC1beta in the induction of mitochondrial gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2515 GDS3197

Platform:

GPL1261

12 Samples

Download data: CEL

Analysis of livers of animals bearing a hypomorphic PGC-1beta mutation. PGC-1beta is a transcriptional coactivator that stimulates mitochondrial biogenesis and respiration of cells. Results provide insight into the function of PGC-1beta in the liver.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE6210

6 Samples

Download data: CEL

Analysis of quadriceps muscles of animals bearing a hypomorphic PGC-1beta mutation. PGC-1beta is a transcriptional coactivator that stimulates mitochondrial biogenesis and respiration of cells. Results provide insight into the function of PGC-1beta in the skeletal muscle.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE6210

6 Samples

Download data: CEL

(Submitter supplied) The transcriptional coactivator peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) is a chief activator of the mitochondrial and metatolic program in skeletal muscle (skm) and prevents atrophy. Here we tested whether PGC-1α overexpression could restructure the transcriptome and metabolism of cultured human skeletal myotubes, which display an athropic phenotype. An oligonucleotide microarray analysis was used to reveal PGC-1α effects on the whole transcriptome, and the possible impact on fuel metabolism reprogramming was examined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

6 Samples

Download data: TXT

(Submitter supplied) Mitochondrial oxidative function is tightly controlled to maintain energy homeostasis in response to nutrient and hormonal signals. An important cellular component in the energy sensing response is the target of rapamycin (TOR) kinase pathway; however whether and how mTOR controls mitochondrial oxidative activity is unknown. Here, we show that mTOR kinase activity stimulates mitochondrial gene expression and oxidative function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) The homeodomain transcription factor Prep1 was previously shown to regulate insulin sensitivity. Our aim was to study the specific role of Prep1 for the regulation of energy metabolism in skeletal muscle. Muscle specific ablation of Prep1 resulted in increased expression of respiratory chain subunits. This finding was consistent with an increase in mitochondrial enzyme activity without affecting mitochondrial volume fraction as assessed by electron microscopy. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

8 Samples

Download data: TXT

(Submitter supplied) Type 2 diabetes mellitus (DM) is characterized by insulin resistance and pancreatic beta-cell dysfunction. In high-risk subjects, the earliest detectable abnormality is insulin resistance in skeletal muscle. Impaired insulin-mediated signaling, gene expression, and glycogen synthesis, and accumulation of intramyocellular triglycerides have all been linked with insulin resistance, but no specific defect responsible for insulin resistance and DM has been identified in humans. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL80

20 Samples

Download data: CEL