GEO DataSets

(Submitter supplied) Transcriptional profling of a Listeria monocytogenes under nisin treatment comparing ctsR mutant and wild type

Organism:

Listeria monocytogenes str. Scott A; Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL6489

4 Samples

Download data: TXT

(Submitter supplied) Transcriptional profling of a Listeria monocytogenes under pressure comparing ctsR mutant and wild type

Organism:

Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL6489

4 Samples

Download data

(Submitter supplied) The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator CtsR, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DctsR log phase cells were compared to both wt and ictsR-mcsA log phase cells grown with 0.5mM IPTG to identify CtsR-dependent genes.We identified 62 CtsR-dependent genes that showed significant expression ratios (adj. more...

Organism:

Listeria monocytogenes; Listeria innocua

Type:

Expression profiling by array

Platform:

GPL5029

2 Samples

Download data: GPR

(Submitter supplied) Here we use RNA sequencing to identify the regulon of the two-component system regulator VirR under normal growth conditions. We generated an in-frame deletion of the virR gene and extracted RNA from this strain grown to mid-log phase in LB as well as the 10403S parental strain. We then analyzed differential expression of transcripts between these two strains using RNA-seq

Organism:

Listeria monocytogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21330

12 Samples

Download data: TXT

(Submitter supplied) To characterize regulons of alternative sigma factor SigH, SigL, and SigC in Listeria monocytogenes, in-frame mutant strains were created in the 10403S background. Regulons controlled by these 3 alternative sigma factors were characterized by whole-genome microarrays. The L. monocytogenes 10403S wild type and sigma factor null mutation strains were grown at 37 °C to stationary phase (defined in this study as growth to OD600 = 1.0, followed by incubation for an additional 3 h) prior to RNA isolation. more...

Organism:

Listeria monocytogenes; Listeria innocua

Type:

Expression profiling by array

Platform:

GPL5029

5 Samples

Download data: GPR, TXT

(Submitter supplied) The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator HrcA, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DhrcA stationary phase cells were compared to wt to identify hrcA-dependent genes. We identified 61 HrcA-dependent genes that showed significant expression ratios (adj. more...

Organism:

Listeria monocytogenes; Listeria innocua

Type:

Expression profiling by array

Platform:

GPL5029

1 Sample

Download data: GPR

(Submitter supplied) The formation of Listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. A microarray comparison of L. monocytogenes between the transcriptome of the strong biofilm forming strain (Bfms) Scott A and the weak biofilm forming (Bfmw) strain F2365 was conducted to identify genes potentially involved in biofilm formation. Among 951 genes with significant difference in expression between the two strains, a GntR-family response regulator encoding gene (LMOf2365_0414), designated lbrA, was found to be highly expressed in Scott A relative to F2365. more...

Organism:

Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL5854

6 Samples

Download data: GPR, MEV

(Submitter supplied) Analysis of L. monocytogenes ptsH, hprK and ccpA mutants defective in carbon catabolite repression (CCR) control revealed significant alterations in the expression of PrfA-dependent genes. The hprK mutant showed high up-regulation of PrfA-dependent virulence genes upon growth in glucose-containing media whereas expression of these genes was even slightly down-regulated in the ccpA mutant when compared to the wild-type strain. more...

Organism:

Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL4418

10 Samples

Download data

(Submitter supplied) The extensively studied intracellular pathogen, L. monocytogenes, is an ideal model for identifying small-molecule agents for treating bacterial infections. By selecting specific biological targets in L. monocytogenes, which are common to Gram-positive pathogens, we could extrapolate drug discovery information derived from this well-studied bacterium. Attenuating the pathogen’s virulence and stress response attributes without killing it, eliminates selective pressure caused by disruption of essential gene functions (as done by current antibiotics) and reduces the likelihood of developing microbes that are impervious to the effects of antibiotics. more...

Organism:

Listeria innocua; Listeria monocytogenes 10403S; Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL5029

1 Sample

Download data: GPR

(Submitter supplied) Purpose: The objective of the present study was to refine the PrfA regulatory network using RNA-seq to characterize the L. monocytogenes transcriptome in a wt, ∆prfA, and prfA* under PrfA-inducing conditions. Methods: Sequence reads were aligned to the L. monocytogenes 10403S genome using the BWA-MEM algorithm, version 0.7.3a. The data for coverage per base on the sense and antisense strands were analyzed separately using SAMtools. more...

Organism:

Listeria monocytogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26477

9 Samples

Download data: TXT

(Submitter supplied) Listeria monocytogenes strain 10403S has been studied extensively for stress response activity toward multiple stressors (acid, osmotic, cold, high temperature, etc.) as well as multiple stress regulons (SigB, CtsR, HrcA, etc.). Here we aimed to determine the transcriptional response of Listeria monocytogenes in early log phase towards the strong oxidative stress imposed by ClO2. The elucidation of such a response allows for further a more completel understanding of the mechanism of inactivation by sanitizers, specifically ClO2.

Organism:

Listeria monocytogenes; Listeria innocua

Type:

Expression profiling by array

Platform:

GPL5029

4 Samples

Download data: GPR, TXT

(Submitter supplied) Iron is required by almost all bacteria but concentrations of iron above the physiological levels are toxic. In bacteria, intracellular iron is regulated mostly by ferric uptake regulator, Fur or a similar functional protein. Iron limitation results in regulation of number of genes, including those involved in iron uptake process. A subset of the genes under the control of Fur is called Fur regulon. more...

Organism:

Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL7073

8 Samples

Download data: TXT

(Submitter supplied) This study investigated the alternative gene, protein expression and morphological changes of L. monocytogenes 08-5923 following 15min, 4h and 30h exposure to bacteriocin carnocyclin A (cclA). The microarray experiment studied the gene expression of untreated and treated Listeria cells following 4h exposure to cclA.

Organism:

Listeria monocytogenes; Listeria monocytogenes 08-5923

Type:

Expression profiling by array

Platform:

GPL18538

6 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Information regarding the Alkaline Tolerance Response (AlTR) in Listeria monocytogenes is very limited. In this study, L. monocytogenes was shown to exhibit a significant adaptive alkaline tolerance response (AlTR) following a 1-h exposure to mild alkaline (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Treatment of adapted cells with protein synthesis inhibitor chloramphenicol has revealed that AlTR is at least partially protein-dependent. more...

Organism:

Listeria monocytogenes; Listeria monocytogenes 10403S

Type:

Expression profiling by array

Platform:

GPL5170

1 Sample

Download data: TAV

(Submitter supplied) Transcriptome profiles of control Lactobacillus plantarum WCFS1 cells were compared with ctsR, hrcA, and ctsR-hrcA deletion mutants grown in MRS media at 28 and 40 degrees Celcius.

Organism:

Lactiplantibacillus plantarum; Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL13984

24 Samples

Download data: TXT

(Submitter supplied) To investigate the response of S. pneumoniae to three distinct antimicrobial peptides (AMPs), i.e. bacitracin, nisin and LL-37, transcriptome analysis of challenged bacteria was performed. Only a limited number of genes was found to be up- or down-regulated in all cases. Several of these common highly induced genes were chosen for further analysis, i.e. SP0385-0387, SP0912-0913, SP0785-0787, SP1714-1715 and the blp cluster. more...

Organism:

Streptococcus pneumoniae D39; Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL9459

6 Samples

Download data: TXT

(Submitter supplied) Listeria monocytogenes causes listeriosis, an infectious and potentially fatal disease of animals and humans. A diverse network of transcriptional regulators, including LysR-type catabolite control protein C (CcpC), is critical for the survival of L. monocytogenes and its ability to transition into the host environment. In this study, we explored the physiological and genetic consequences of deleting ccpC and the effects of such deletion on the ability of L. more...

Organism:

Listeria monocytogenes serotype 4b str. F2365

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34483

9 Samples

Download data: TXT

(Submitter supplied) Transcriptome profiles of control Lactobacillus plantarum WCFS1 cells were compared with 8% ethanol adapted cells and with 10 min or 30 min 8% ethanol shocked cells.

Organism:

Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL4318

12 Samples

Download data: TXT

(Submitter supplied) Alkali stress is an important means of inactivating undesirable pathogens in a wide range of situations, ranging from environmental cleaning of food processing environments, to the phagolysosomal killing of cells engulfed by mammalian phagocytes. Unfortunately, L. monocytogenes can launch an alkaline tolerance response (AlTR), significantly increasing persistence of the pathogen in such environments. more...

Organism:

Listeria monocytogenes 10403S; Listeria monocytogenes

Type:

Expression profiling by array

Platform:

GPL5170

4 Samples

Download data: TAV

(Submitter supplied) Using RNA-Seq we show that the PadR-type transcriptional repressor Lmo0599 (LltR) mainly regulates expression of the lmo0599-0600-0601 operon.

Organism:

Listeria monocytogenes EGD-e

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19076

6 Samples

Download data: CSV, TXT