GEO DataSets

(Submitter supplied) The Insm1 gene encodes a zinc finger factor expressed in many endocrine organs. We show here that Insm1 is required for differentiation of all endocrine cell types in the pituitary. Thus, in Insm1 mutant mice, hormones characteristic of the different pituitary cell types (thyroid, follicle and melanocyte stimulating hormone, adrenocorticotrope hormone, growth hormone and prolactin) are absent or produced at markedly reduced levels. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5066

Platform:

GPL6885

16 Samples

Download data: TXT

Analysis of pituitary glands from embryonic day 17.5 mutants deficient for Insm1. The Insm1 gene encodes a zinc finger factor expressed in many endocrine organs. Results provide insight into the role of Insm1 in the development of the pituitary gland.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL6885

Series:

GSE46139

16 Samples

Download data

(Submitter supplied) The anterior pituitary is comprised of five major hormone-secreting cell types which differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the neuroendocrine system were produced and used to identify genes with potential involvement in onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription during chick embryonic development. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1952

Platform:

GPL1744

16 Samples

Download data: TIFF

(Submitter supplied) A major research focus in our laboratory is the development of the chicken pituitary gland. As this tissue does not yield sufficient RNA to analyze using cDNA microarrays, mRNA must be amplified by reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter site followed by in vitro transcription using T7 RNA polymerase. Therefore, this preliminary study investigating differential gene expression between embryonic day 17, when all the major cell types in the chick anterior pituitary gland have differentiated, and post-hatch day 3 was conducted to verify that amplified antisense RNA could be used in conjunction with our neuroendocrine microarrays containing 5,128 unique cDNAs to detect changes in gene expression. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1951

Platform:

GPL1744

6 Samples

Download data: TIFF

(Submitter supplied) Spotted amplified cDNA array on glass. Produced with cDNA clones expressed in the chicken neuroendocrine system. Tissues used to produced the cDNA library included the hypothalamus, anterior pituitary gland, and pineal gland. 7200 spots Keywords = spotted Keywords = cDNA

Organism:

Gallus gallus

2 DataSets

2 Series

22 Samples

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Analysis of embryonic pituitary glands at various ages from day 10 to day 17 using a neuroendocrine system specific cDNA microarray. Results identify genes potentially involved in the onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 4 age sets

Platform:

GPL1744

Series:

GSE3227

16 Samples

Download data: TIFF

Analysis of pituitary glands from day 17 embryos and post-hatch day 3 animals using a neuroendocrine system specific cDNA microarray. Major cell types in the anterior pituitary gland have differentiated at embryonic day 17. Results used to validate the method of amplifying RNA from pituitary glands.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 2 age sets

Platform:

GPL1744

Series:

GSE3226

6 Samples

Download data: TIFF

(Submitter supplied) Genome-wide Insm1 binding sites analysis revealed an overrepresentation of sequences predicted to bind bHLH and/or forkhead factors, and further analyses demonstrated that most Insm1 sites in the genome are co-occupied by Neurod1 and Foxa2. Binding regions co-occupied by all three factors but not single Insm1 sites explained a significant fraction of gene expression changes in Insm1 mutant β-cells. Together, our data provide evidence that an Insm1, Neurod1 and Foxa2 network maintains a mature gene expression program in β-cells.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: TSV

(Submitter supplied) The zinc finger factor Insm1 is known to regulate differentiation of pancreatic β cells during development, Here we show that Insm1 is essential for the maintenance of functionally mature pancreatic β cells in mice. We used microarrays to analyse the global gene expression after deletion of insm1 in adult pancreatic β cells and identified functional important genes and immature islets releated genes deregulated in the mutatant islets.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) Here we find that ZNF750 activates epidermal differentiation genes and represses progenitor genes as part of two distinct protein complexes

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

4 Samples

Download data: TXT

(Submitter supplied) T-3775440 is an irreversible inhibitor of the chromatin demethylase LSD1. Here we describe the anti-cancer effects and mechanism of action of T-3775440 in small cell lung cancer (SCLC). T-3775440 inhibited proliferation of SCLC cells in vitro and retarded SCLC tumor growth in vivo. Our results argue that LSD1 plays an important role in neuroendocrine-associated transcription and cell proliferation of SCLC via interactions with the SNAG domain proteins INSM1 and GFI1B. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16699

18 Samples

Download data: TXT

(Submitter supplied) Jarid2, a member of the Jumanji family of proteins, is a developmental regulator that is necessary for proper mouse development and stem cell differentiation. To investigate the specific functions of Jarid2 during pancreas development, we generated pancreas-specific Jarid2 mutants. We used microarrays to determine gene expression changes resulting from deletion of the Jarid2 gene in the pancreas.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) We performed gene expression pofiling of Zeb2cKO and control sciatic nerves and identified significantly changed genes ZEB2 is also known as SIP1

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: TXT, XLSX

(Submitter supplied) Developmental progression depends on temporally defined changes in gene expression mediated by transient exposure of lineage intermediates to signals in the progenitor niche. To identify a possible contribution of cell-intrinsic epigenetic mechanisms to signal-induced transcriptional responses, we manipulated the signaling environment and activity of the histone demethylase LSD1 during stepwise differentiation of gut tube intermediates into pancreatic endocrine cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20301

63 Samples

Download data: BIGWIG

(Submitter supplied) To analyze stem/progenitor cell function, we purified hepatocytes derived from adult livers and fetal hepatoblasts derived from embryonic day 13 livers.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) The anterior pituitary gland plays central roles in body growth, reproduction, metabolism and the stress response. In this study, we performed single-cell RNA-sequencing (scRNA-seq) of 4,113 individual cells from human fetal pituitaries. We characterized divergent developmental trajectories with distinct transitional intermediate states in five hormone-producing cell lineages. Furthermore, we characterized the cellular heterogeneity of pituitary stem cells, identifying a hybrid epithelial/mesenchymal state and an early-to-late state transition. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

89 Samples

Download data: CSV, TXT, XLSX

(Submitter supplied) Delamination of neural progenitor cells (NPCs) from the ventricular surface is a crucial prerequisite to form the subventricular zone, the germinal layer linked to the expansion of the mammalian neocortex in development and evolution. Here, we dissect the molecular mechanism by which the transcription factor Insm1 promotes the generation of basal progenitors (BPs). Insm1 protein is most highly expressed in newborn BPs in mouse and human developing neocortex. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) High mobility group proteins are chromatin regulators with essential functions in development, cell differentiation and cell proliferation. We have shown that the high mobility group protein HMG20A copurifies with the histone reader PHF14. To understand the common functions of HMG20A and PHF14 proteins in regulating the transcriptome, we have performed RNA-Seq expression analysis following depletion of each protein in the triple-negative breast cancer cell line MDA-MB-231. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: TSV