GEO DataSets

(Submitter supplied) S. aureus biofilms are associated with the organism's ability to cause disease. Biofilm associated bacteria must cope with the host's innate immune system. We used commercially available Affymetrix S. aureus GeneChips to compare the gene expression properties of 4 and 6 day established biofilms following short (1 hr)- and long (24 hr)- term exposure to macrophages and neutrophils.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

35 Samples

Download data: CEL, CHP

(Submitter supplied) To measure S. aureus gene expression during growth phase and/or S. aureus–PMN interaction. Keywords: other

Organism:

Streptococcus pyogenes; Chlamydia trachomatis; Borreliella burgdorferi; Yersinia pestis; Coxiella burnetii; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL2129

159 Samples

Download data

(Submitter supplied) The two immune cell populations Myeloid-derived suppressor cells (MDSCs), monocytes (MONO) and neutrophils (PMNs) are difficult to differentiate because of shared surface marker expression. Here we utilize the integrin receptor CD11b combined with conventional Ly6G and Ly6C expression to more accurately separate cellular populations via FACS. Then we apply high-throughput RNA Sequencing to Ly6G+Ly6C+CD11bhigh MDSC, Ly6G+Ly6C+CD11blow PMN and Ly6G-Ly6C+ monocyte populations. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: CSV, TXT

(Submitter supplied) Staphylococcus aureus is an opportunistic pathogen that colonizes the anterior nares of about 30-50% of the population. Colonization is most often asymptomatic, however, self-inoculation through inhalation, ingestion, or an open wound, can give rise to potentially fatal infections of the deeper tissues and blood. Like all bacteria, S. aureus is able to sense and respond to environmental cues and modify gene expression to adapt to specific environmental conditions. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25144

9 Samples

Download data: XLSX

(Submitter supplied) The WalKR regulon was studied by comparing genes expression in a strain producing a constitutively active WalR regulator (WalRc) versus the wild type strain carrying the empty expression vector. Hybridizations were performed with three independent biological replicates. Our data allowed to identify 165 genes differentially expressed in the walRc expressing strain with a P-value ≤ 0.05 using Z-test and a threshold value of two-fold change in transcriptional levels. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325

Type:

Expression profiling by array

Platform:

GPL11308

6 Samples

Download data: PAIR

(Submitter supplied) These cultures were grown to examine the differences in Agr-regulated virulence factor gene expression between wild-type S. aureus FRI1169 and a non-hemolytic variant isolated from a biofilm inoculated with FRI1169. The study is described more thoroughly in the paper "Generation of virulence factor variants in Staphylococcus aureus biofilms", Yarwood et al., J. Bacteriol. 2007. Keywords: Wild-type versus mutant comparison, single time-point

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

4 Samples

Download data: CEL, CHP

(Submitter supplied) The purpose of this study was to compare the global, growth phase-dependent transcriptional profiles of two isolates of Staphylococcus aureus. One isolate is a prototypic laboratory strain named RN6390, and has been used frequently as a model organism for study of staphylococcal physiology and virulence. However, recent studies indicate that RN6390 is not, in general, genotypically or phenotypically representative of clinical isolates of Staphyloccos aureus. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Dataset:

GDS2370

Platform:

GPL4047

16 Samples

Download data

Analysis of Staphylococcus aureus UAMS-1 virulent clinical isolate and its isogenic agr and sarA mutants. agr and sarA genes encode global regulators important in virulence factor production and biofilm formation. Results provide insight into the differences between clinical and laboratory strains.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array, count, 2 development stage, 4 genotype/variation, 2 strain sets

Platform:

GPL4047

Series:

GSE5466

16 Samples

Download data

(Submitter supplied) Successful host defense against pathogens requires innate immune recognition of the correct pathogen associated molecular patterns (PAMPs) by pathogen recognition receptors (PRRs) to trigger the appropriate gene program tailored to the pathogen. While many PRR pathways have been shown to contribute to the innate immune response to specific pathogens, the relative importance of each pathway for the complete transcriptional program elicited has not been examined in detail. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

83 Samples

Download data: BEDGRAPH

(Submitter supplied) Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) We examined the differential gene expression of Staphylococcus epidermidis and Staphylococcus epidermidis in dual species biofilms. Therefore, we performed RNA-Seq on single and dual species biofilms and we compared the gene expression levels in dual species biofilms to those in single species biofilms.

Organism:

Staphylococcus aureus; Staphylococcus epidermidis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21653 GPL17452 GPL21652

9 Samples

Download data: XLSX

(Submitter supplied) From a Caenorhabditis elegans - S. aureus anti-infective screen, we identified benzimidazole derivative C162 as one of the potential anti-infective candidate that rescued the infected-nematodes from infection. This compound was found to exhibit anti-biofilm activity against S. aureus. To investigate the transcriptome profile of S. aureus in response to benzimidazole derivative C162, a genome-wide transcriptome analysis was performed on C162-treated S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

6 Samples

Download data: CEL

(Submitter supplied) Microarray expression profiling of S. aureus lavaged from murine lungs after residence times between 30 minutes and 6 hours compared to post-exponential phase or early log phase growth in Luria-Betani broth.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL4047

21 Samples

Download data: CEL

(Submitter supplied) Background: Staphylococcus aureus is a major pathogen of humans and animals and rapidly emerging antibiotic-resistant strains have further increased the severity of this health issue. Host genetics influence susceptibility to S. aureus infections, and genes determining infection outcome should be identified to analyze immune-based therapies as an alternative to antibiotics. Here, we used outbred animals from a divergent selection on susceptibility towards Staphylococcus infection to explore host immunogenetics. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL10427

44 Samples

Download data

(Submitter supplied) To investigate biofilm-leukocyte crosstalk, we performed RNA-seq to evaluate how the S. aureus biofilm transcriptome was altered following exposure to G-MDSCs or macrophages in vitro. Interestingly, each immune cell type elicited a distinct transcriptional response.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

10 Samples

Download data: XLSX

(Submitter supplied) scRNA-seq of phagocytic vs. non phagocytic immune cells during acute S. aureus craniotomy infection

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: H5

(Submitter supplied) Infection is a devastating post-surgical complication, often necessitating additional procedures and prolonged antibiotic therapy. This is especially relevant for craniotomy and prosthetic joint infections, both of which are characterized by biofilm formation on the bone or implant surface, respectively, with S. aureus representing a primary cause. The effectiveness of immune responses to these infections is predicated on both host- and pathogen-derived signals in the infection microenvironment. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: TXT

(Submitter supplied) Neurosurgeries complicated by bacterial infection are often associated with poor clinical prognosis and prolonged treatment strategies involving multiple surgeries, placing additional morbidity on an already fragile patient population. Craniotomy is one of the most common neurosurgical procedures with an infection rate of 1-7%; however, the cellular and molecular signatures associated with craniotomy infection in human subjects have not yet been investigated. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

14 Samples

Download data: H5

(Submitter supplied) Better understanding of S. aureus throat colonization in the presence of other competing/coexisting microbes may provide insight into S. aureus adaptation to the human throat and recurrence of infection. In this work, we explore the responses triggered by the encounter between two common throat bacteria, S. aureus and S. anginosus, in the presence of human tonsillar epithelial cells. We performed an in vitro coculture experiment followed by RNA sequencing. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28116

12 Samples

Download data: FASTA, TXT