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Series GSE50675 Query DataSets for GSE50675
Status Public on Dec 01, 2013
Title Global transcriptome analysis of Staphylococcus aureus biofilms in response to innate immune cells
Organism Staphylococcus aureus
Experiment type Expression profiling by array
Summary S. aureus biofilms are associated with the organism's ability to cause disease. Biofilm associated bacteria must cope with the host's innate immune system.
We used commercially available Affymetrix S. aureus GeneChips to compare the gene expression properties of 4 and 6 day established biofilms following short (1 hr)- and long (24 hr)- term exposure to macrophages and neutrophils.
 
Overall design S. aureus strain USA300 LAC biofilms where formed for 4 or 6 days. Established biofilms were then exposed to macrophages for 1 or 24 hr. Alternatively, biofilms were exposed to neutrophils for 1 or 4 hr. Total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of Macrophages and Neutrophils on established S. aureus biofilms.
 
Contributor(s) Dunman PM, Kielian TL
Citation(s) 24042108
Submission date Sep 06, 2013
Last update date Jan 09, 2014
Contact name Paul Dunman
E-mail(s) [email protected]
Phone 585-276-5700
Organization name University of Rochester
Department Microbiology and Immunology
Street address 601 Elmwood Avenue
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
 
Platforms (1)
GPL1339 [S_aureus] Affymetrix S. aureus Genome Array
Samples (35)
GSM1226122 4 day biofilm + 1 hr biological replicate 1
GSM1226123 4 day biofilm + 1 hr biological replicate 2
GSM1226124 4 day biofilm + 1 hr biological replicate 3
Relations
BioProject PRJNA218370

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50675_RAW.tar 41.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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