GEO DataSets

(Submitter supplied) Deinococcus deserti is a desiccation- and radiation-tolerant desert bacterium. Differential RNA sequencing was performed to explore the specificities of its transcriptome. Strikingly, for 1174 (60%) mRNAs the transcription start site was found exactly at (916 cases, 47%) or very close to the translation initiation codon AUG or GUG. Such proportion of leaderless mRNAs, which may resemble ancestral mRNAs, is unprecedented for a bacterial species. more...

Organism:

Deinococcus deserti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18445

4 Samples

Download data: TXT

(Submitter supplied) We report genome-wide transcriptional UV response in Deincoccus gobiensis

Organism:

Deinococcus gobiensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13495

2 Samples

Download data: TXT

(Submitter supplied) This bacterial genome encodes pyrroloquinoline quinone (PQQ) synthase enzyme required for the synthesis of PQQ in bacteria. The gene pqqE encoding PQQ synthase was annotated in Deinococcus radiodurans R1 genome and synthesis of PQQ was shown in this bacterium. PQQ roles as an antioxidant and as an inducer of protein kinase in bacterial system was shown. The pqqE disuption mutant showed several folds decrease in gamma radiation tolerance of wild type cells and strong impairment of DSB repair. more...

Organism:

Deinococcus radiodurans; Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Type:

Expression profiling by array

Platform:

GPL8947

2 Samples

Download data: GPR

(Submitter supplied) Bacterial cells lacking pyrroloquinoline quinone (PQQ) were sensitive to gamma radiation as compared to wild type cells. Deinococcus radiodurans genome encode for five putative PQQ binding STK domain-containing proteins. The comparison of gamma radiation response of all five-deletion mutants with wild type suggested that dr2518 mutant was hypersensitive to gamma radiation as compared to wild type and other mutants. more...

Organism:

Deinococcus radiodurans; Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Type:

Expression profiling by array

Platform:

GPL8947

2 Samples

Download data: GPR

(Submitter supplied) The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in their promoter regions. Here, we showed that addition of zinc ions to purified apo-IrrE, and short exposure of Deinococcus cells to zinc ions, resulted in cleavage of DdrO in vitro and in vivo, respectively. more...

Organism:

Deinococcus deserti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18445

4 Samples

Download data: TXT

(Submitter supplied) Most eukaryotic mRNAs are enzymatically processed before they are translated, but less is known about mRNA processing in prokaryotes. While bacterial rRNAs and tRNAs are known to be highly processed, only a handful of bacterial mRNAs have been shown to be processed by ribonucleases, typically altering transcript stability. We hypothesized that mRNA processing might be more widespread in prokaryotes and constitute a previously underappreciated layer of post-transcriptional regulation. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL19272 GPL15138

9 Samples

Download data: TXT

(Submitter supplied) This data contained within this entry was produced as part of a study that included differential RNA-sequencing and microarray analysis. The results of the latter two have also been deposited in GEO

Organism:

Cutibacterium acnes KPA171202

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17154

2 Samples

Download data: TXT

(Submitter supplied) The differential RNA-seq data contained within this entry is complemented by global RNA-seq and microarray data, which is also deposited in GEO.

Organism:

Cutibacterium acnes KPA171202

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17147

4 Samples

Download data: TXT

(Submitter supplied) Cronobacter sakazakii is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food formula, often leading to high mortality rates. Here, we used RNA-seq to provide physiological and transcriptional insights into the survival of C. sakazakii in desiccated conditions. Our RNA-seq data shows that about 22% of the total C. sakazakii genes were significantly up-regulated and 9% were down-regulated during desiccation survival. more...

Organism:

Cronobacter sakazakii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25429

6 Samples

Download data: TXT