GEO DataSets

(Submitter supplied) Ten volunteers underwent 13 research IVM cycles. Immature oocytes were retrieved after mild ovarian stimulation with gonadotropins but no hCG ovulation trigger, in vitro matured and fertilized using ICSI. Oocyte maturation rate in vitro was 47% (94/199), the mean fertilization rate was 71% (67/94) and 37% (25/67) of mature oocytes developed into a morphologically good quality embryo on day 3 after ICSI, after which the blastomeres were analysed by single-cell aCGH. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL17760

123 Samples

Download data: TXT

(Submitter supplied) Chromosomal instability (CIN) occurs at high frequency during early in vitro embryogenesis and is known to be associated with early embryonic loss in humans. The chromosomal stability of in vivo-conceived cleavage stage embryos largely remains unknown. Here, we applied haplotyping and copy number profiling to investigate genomic architecture of 171 single bovine blastomeres and to compare the nature and frequency of CIN between in vivo embryos, in vitro embryos produced from ovum pick up with ovarian stimulation (OPU-IVF), and in vitro produced embryos from in vitro matured oocytes without ovarian stimulation (IVM-IVF). more...

Organism:

Bos taurus

Type:

SNP genotyping by SNP array

Platform:

GPL21267

236 Samples

Download data: IDAT

(Submitter supplied) Seventy blastomeres from fourteen frozen-thawed supernumerary human preimplantation embryos were disassociated and genomic was amplified using Multiple Displacement Amplification. BAC array-CGH was performed on the amplified products.

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL14983

70 Samples

Download data: TXT

(Submitter supplied) In total, 240 single blastomeres from nine top-quality day-4 embryos frozen at day 3 of development and four fresh top-quality day-4 embryos that had one-cell biopsy on day 3 for preimplantation genetic diagnosis (PGD) were collected. Blastomeres' DNA was amplified using SurePlex DNA Amplification System (BlueGnome, Cambridge, UK) . Array-CGH was carried out using 24Sure Cytochip microarrays following the standard protocol (BlueGnome, www.cytochip.com).

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL15832

240 Samples

Download data: TXT

(Submitter supplied) Study Question: What effects do maternal age and oocyte maturation stage have on the human oocyte transcriptome that may be associated with oocyte developmental potential? Summary Answer: Although polyadenylated transcript abundance changes during human oocyte maturation irrespective of age, young (YNG) and advanced maternal age (AMA) metaphase II (MII) oocytes exhibit divergent transcriptomes. What is known already: Maternal age and maturation stage affect oocyte polyadenylated transcript abundance in human oocytes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

20 Samples

Download data: CSV

(Submitter supplied) Gene expression analysis in cc from COCs with different chromatin configuration (GV0-3)

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: TXT

(Submitter supplied) Nuclear abnormalities are commonly found in human IVF embryos and are associated with DNA damage, aneuploidy, and decreased developmental potential.

Organism:

Homo sapiens

Type:

SNP genotyping by SNP array; Genome variation profiling by SNP array

Platform:

GPL3718

72 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Objective: To study post-hatching development after transfer of morphologically good quality embryos that survived in vitro maturation under lipolytic conditions using a well-established and recognized bovine model. Design: prospective study with a crossover design. Setting: research laboratory. Animals: 8 healthy multiparous non-lactating Holstein cows used in repeated embryo transfers. Intervention(s): Bovine cumulus oocyte complexes (COCs) were in vitro matured in the presence of either 1) a combination of elevated pathophysiological concentration of palmitic acid (PA) and physiological concentrations of stearic acid (SA) and oleic acid (OA) (HIGH PA), or 2) physiological concentrations of PA, SA and OA (BASAL). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22316

53 Samples

Download data: TXT

(Submitter supplied) We highlighted the effects of age and ovarian responses on rescue IVM oocyte quality.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

21 Samples

Download data: TXT, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23949 GPL19129

29 Samples

Download data: TXT

(Submitter supplied) Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19129 GPL23949

11 Samples

Download data: TXT

(Submitter supplied) Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23949

18 Samples

Download data: TXT

(Submitter supplied) Oocyte vitrification has been introduced into clinical settings without pre-clinical safety testing. In this study, we analyzed the safety of human oocyte

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL17760

48 Samples

Download data: TXT

(Submitter supplied) Study question: What is the relative effect of common environmental and biological factors on transcriptome changes during human preimplantation development? Summary answer: Developmental stage and maternal age had a larger effect on the global gene expression profile of human preimplantation embryos than the culture medium or oxygen concentration used in in vitro culture. What is known already: Studies on mouse and bovine embryos have shown that different conditions in the in vitro culture of embryos can lead to changes in transcriptome profiles. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17513

37 Samples

Download data: TXT

(Submitter supplied) Find the different expression profiles between human oocytes IVM with growth hormone and human oocytes without growth hormone. We collected human oocytes cultured by IVM for 24h with growth hormone or not, prepared single cell cDNAs, and sequenced them by Illumina HiSeq Xten.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

6 Samples

Download data: TXT

(Submitter supplied) The oocytes presenting SERa are considered dysmorphic and have been associated with reduced embryological outcome and an increased risk of congenital anomalies. Therefore, in 2011 the Alpha/ESHRE Consensus strongly recommended not to inseminate MII oocytes displaying SERa (SERa+). Not all Centers follow this recommendation and some Authors reported that SERa+ can lead to healthy newborns. Moreover, no experimental data about the effect of SERa presence on the oocyte molecular status are available. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

6 Samples

Download data: TXT

(Submitter supplied) The mean age of women seeking infertility treatment has gradually increased over recent years. This has coincided with the emergence of in vitro maturation (IVM), a method used in assisted reproductive technology for patients with special requirements. However, when compared with conventional in vitro fertilization, IVM is associated with poor embryonic development potential and low live birth rates, thus limiting the widespread application of this technique. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: TXT

(Submitter supplied) Embryonic chromosome aberrations cause birth defects and reduce human fertility. However, neither their nature nor incidence are known. Here, we develop a method to assess genome-wide copy number variation and loss of heterozygosity in single cells and apply it to screen blastomeres from in vitro fertilized preimplantation embryos. Complex patterns of chromosome-arm imbalances or segmental deletions, duplications or amplifications that were reciprocal in sister blastomeres were detected in a large proportion of the embryos. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array; Genome variation profiling by SNP array; SNP genotyping by SNP array

Platforms:

GPL6929 GPL3718 GPL6928

290 Samples

Download data: CEL, CHP, GPR

(Submitter supplied) We aimed to reveal gene expression profiles of human oocytes at each maturation stage by single cell RNA-seq analyses. We investigated transcriptomes of immature oocytes at the germinal vesicle (GV) stage, maturating oocytes at the metaphase I (MI) stage and in vitro matured oocytes at the metaphase II (MII) stage.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

19 Samples

Download data: XLSX

(Submitter supplied) Single cell transcriptomic composition of in vivo matured (IVO) MII oocytes and maturation deficient (MD) oocytes discloses the aberrant expression level of spliceosomal proteins, accompanying with differential alternative splicing (DAS) events.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: TXT