GEO DataSets

(Submitter supplied) NaCl-resistant Saccharomyces cerevisiae mutant was obtained by evolutionary engineering. EMS mutagenized culture was used as the initial population for the selection procedure. Gradually increasing levels of NaCl stress was applied through 40 successive batch cultivations. The reference strain could not grow even at 0.85 M NaCl whereas this mutant was shown to be resistant up to 1.45 M NaCl concentration. more...

Organism:

Saccharomyces cerevisiae; Saccharomyces cerevisiae CEN.PK113-7D

Type:

Expression profiling by array

Platform:

GPL16244

6 Samples

Download data: TXT

(Submitter supplied) Nickel-resistant Saccharomyces cerevisiae mutant was obtained by evolutionary engineering. The reference strain which was used to select this nickel-resistant mutant could not grow even at 0.5 mM NiCl2 whereas this mutant was shown to be resistant upto 5.3 mM NiCl2 concentration. Whole-genome microarray analysis might be promising to identify the nickel resistance mechanisms in the yeast cells.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL16244

6 Samples

Download data: TXT

(Submitter supplied) Oxidative stress is a key attribute that one should considered when using yeast cells for industrial applications due to its direct impact on yeast growth, viability, and productivity. However, little information is currently available regarding the molecular mechanisms of oxidative stress induction and the antioxidant response to increased reactive oxygen species (ROS) in yeasts. In this study, we generated experimentally evolved and genetically stable oxidative stress-resistant S. more...

Organism:

Saccharomyces cerevisiae

Type:

Non-coding RNA profiling by array

Platform:

GPL22543

6 Samples

Download data: TXT

(Submitter supplied) The needs for rapid and efficient microbial cell factory design and construction are possible through the enabling technology, metabolic engineering, which is now being facilitated by systems biology approaches. Metabolic engineering is often complimented by directed evolution, where selective pressure is applied to a partially genetically engineered strain to confer a desirable phenotype. The exact genetic modification or resulting genotype that leads to the improved phenotype is often not identified or understood to enable further metabolic engineering. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

8 Samples

Download data: CEL

(Submitter supplied) Phenylethanol-resistant S. cerevisiae mutants were obtained by using evolutionary engineering strategy. Briefly, a chemically mutagenized culture was used as the initial population for the selection procedure. Gradually increasing levels of phenylethanol stress was applied through 56 successive batch cultivations. Individual mutants were selected from the final population. The mutant with the highest phenylethanol resistance could resist up to 3 g/L phenylethanol concentrations. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL16244

6 Samples

Download data: TXT

(Submitter supplied) The Saccharomyces cerevisiae MYO1 gene encodes the myosin type II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Deletion of the MYO1 gene prevents actomyosin-driven cytokinesis thereby activating an alternative mechanism that involves the synthesis of a remedial septum. Myo1p deficiency in yeast (myo1) also causes the formation of attached cells, abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, and increased chitin synthesis. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL884

6 Samples

Download data: TXT

(Submitter supplied) The strain bdf1Δ+HAL2 improved salt resistance of bdf1∆. To gain further insight into the mechanism of bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how HAL2 overexpression and HDA1 deletion improves salt resistance. Transcriptomic analysis under salt treatment (0.5 mol.L-1 NaCl for 45 min) was performed using four different strains: bdf1∆, W303, bdf1Δ+HAL2 and bdf1∆hda1∆. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL17201

5 Samples

Download data: TXT

(Submitter supplied) The res (restored cell structure by salinity) mutant exhibits a remarkable growth inhibition and morphological alterations in roots and leaves, which are suppressed when the mutant plants are exposed to salinity. The analysis of mutants like res may be very helpful in understanding how plants are able to coordinate the balance between growth and stress, a fundamental and not fully-understood question of important agronomical implications In order to understand the molecular basis of phenotype recovery and tolerance induced by salt stress in res mutant, we carried out a comparative transcriptomic analysis between res and WT plants. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL21525

24 Samples

Download data: CEL, CHP

(Submitter supplied) In Saccharomyces cerevisiae, glycogen and trehalose are important reserve carbohydrates that accumulate under nutrient limitation in batch cultures. An inherent draw-back of batch studies is that specific growth rate and substrate and product concentrations are variable over time and between cultures. The aim of this present study was to identify the nutritional requirements associated with high accumulation of reserve carbohydrates at a fixed specific growth rate (0.10 h-1) in anaerobic chemostat cultures that were limited by one of five different nutrients (carbon, nitrogen, sulfur, phosphorus or zinc). more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

21 Samples

Download data: CEL, CHP, EXP