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| Status |
Public on Sep 19, 2013 |
| Title |
Transcriptomic analysis of a nickel-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Nickel-resistant Saccharomyces cerevisiae mutant was obtained by evolutionary engineering. The reference strain which was used to select this nickel-resistant mutant could not grow even at 0.5 mM NiCl2 whereas this mutant was shown to be resistant upto 5.3 mM NiCl2 concentration. Whole-genome microarray analysis might be promising to identify the nickel resistance mechanisms in the yeast cells.
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| Overall design |
The reference Saccharomyces cerevisiae strain and the nickel-resistant mutant were grown in minimal medium to an Optical Density (OD600) of 1.00 which correspond to the logarithmic growth phase of the yeast cells. Cultures were harvested and whole RNA isolation was carried out. The experiment was repeated three times.
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| Contributor(s) |
Küçükgöze G, Alkım C, Yılmaz U, Kısakesen HI, Cakar ZP |
| Citation(s) |
23992612 |
| |
| Submission date |
Sep 18, 2013 |
| Last update date |
May 18, 2017 |
| Contact name |
CEREN ALKIM |
| E-mail(s) |
[email protected]
|
| Organization name |
INRA
|
| Street address |
Laboratoire d'Ingénierie des Systèmes Biologiques & des Procédés
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| City |
TOULOUSE |
| ZIP/Postal code |
31077 |
| Country |
France |
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| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
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| Samples (6)
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| GSM1234258 |
Wild Type-without nickel-log phase-rep1 |
| GSM1234259 |
Wild Type-without nickel-log phase-rep2 |
| GSM1234260 |
Wild Type-without nickel-log phase-rep3 |
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| Relations |
| BioProject |
PRJNA219478 |
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