GEO DataSets

(Submitter supplied) In order to decipher the pathways of DHA via cumulus cells (CC) surrounding an oocytes, microarray hybridization of cumulus cells after 4h IVM in the presence or absence of 1µM DHA was performed.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL21724

8 Samples

Download data: TXT

(Submitter supplied) Gene expression analysis in cc from COCs with different chromatin configuration (GV0-3)

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: TXT

(Submitter supplied) Study the effect of recombinant human FSH supplementation in the in vitro maturation medium for an incubation of 6 hrs on the transcriptomics of bovine cumulus cells.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

6 Samples

Download data: TXT

(Submitter supplied) Study the effect of in vitro maturation system on the transcriptomics of bovine cumulus cells during incubation of first 6 hrs.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

6 Samples

Download data: TXT

(Submitter supplied) Cumulus cells, surrounding the oocyte, play a key role in the acquisition of oocyte competence to be fertilized and to sustain early embryo development. Cumulus cells contribute to oocyte development by metabolizing energy substrates such as glutathione that may protect the oocyte from oxidative stress damages. The aim of our study was to compare transcriptomics profiles of cumulus enclosed (CEO) and cumulus denuded (CDO) oocytes after in vitro maturation.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6694

8 Samples

Download data: GPR, TXT

(Submitter supplied) The present study was undertaken to discover molecular markers in bovine cumulus cells predictive of oocyte competence and elucidate their functional significance. Differences in RNA transcript abundance in cumulus cells harvested from oocytes of adult versus prepubertal animals (model of poor oocyte quality) were identified by microarray analysis. Four genes of interest encoding for the lysosomal cysteine proteinases cathepsin B, S, K and Z and displaying greater transcript abundance in cumulus cells surrounding oocytes harvested from prepubertal animals were chosen for further investigation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL325

8 Samples

Download data: TXT

(Submitter supplied) Study the effect of forskolin, dipyridamol and IBMX (here called FID) supplementation in the in vitro maturation medium for an incubation of 6 hrs on the transcriptomics of bovine cumulus cells.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

6 Samples

Download data: TXT

(Submitter supplied) The maturation of an oocyte, the female gamete, during folliculogenesis is highly dependent on the molecular interaction with the somatic cells. Here we generated transcriptome data of single oocytes and the surrounding cumulus cells from antral follicles, and developed an analytical framework to unveil their inter-cellular communication. We identified hundreds of ligand-receptors pairs that can transduce paracrine signaling between oocyte and cumulus. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19172

48 Samples

Download data: TXT

(Submitter supplied) The elaboration of a quality oocyte is integrally linked to the correct developmental progression of cumulus cell phenotype. In humans and non-human primates, oocyte quality is diminished with in vitro maturation. To determine the changes in gene expression in rhesus monkey cumulus cells (CC) that occur during the final day prior to oocyte maturation and how these changes differ between in vitro and in vivo maturation (IVM and VVM), we completed a detailed comparison of transcriptomes using the Affymetrix gene array. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

11 Samples

Download data: CEL

(Submitter supplied) Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA‐seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl-), and (3) oocytes not cleaving (Cl-).

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23295

14 Samples

Download data: TSV

(Submitter supplied) This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

18 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Methylation profiling by array

Platforms:

GPL28869 GPL28870

8 Samples

Download data: TXT

(Submitter supplied) The transcriptome pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs with cocultured of monolayer of GSs was investigated. Genome-wide transcriptome analysis was performed by micro array using EDMA platform.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL28870

4 Samples

Download data: TXT

(Submitter supplied) The DNA methylation pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs was investigated. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

Organism:

Sus scrofa

Type:

Methylation profiling by array

Platform:

GPL28869

4 Samples

Download data: TXT

(Submitter supplied) Pyruvate, the end-product of glycolysis in aerobic conditions, is converted into acetyl-CoA inside the mitochondria of oocytes as a master fuel input for the tricarboxylic acid (TCA) cycle. The citrate generated in the TCA cycle can be directed to the cytoplasm and converted back to acetyl-CoA, being driven to lipid synthesis or, alternatively, being used as the substrate for histones acetylation. This work aimed to verify the impact of pyruvate metabolism on the dynamics of lysine 9 histone 3 acetylation (H3K9ac) and RNA transcription in bovine oocytes during in vitro maturation (IVM). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL13534 GPL10558

48 Samples

Download data: IDAT

(Submitter supplied) Worldwide the rate of infertility is on rise which put increasing pressure on assisted reproductive technology (ART) programs worldwide. Therefore it is important to increase the efficiency of ART. Toward this aim studies has been performed to find the best predictive biomarkers to choose the best germ cells for fertilization and the best embryo for transfer into uterus. However, across different studies no universal markers were found. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

24 Samples

Download data: IDAT, TXT

(Submitter supplied) Worldwide the rate of infertility is on rise which put increasing pressure on assisted reproductive technology (ART) programs worldwide. Therefore it is important to increase the efficiency of ART. Toward this aim studies has been performed to find the best predictive biomarkers to choose the best germ cells for fertilization and the best embryo for transfer into uterus. However, across different studies no universal markers were found. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

24 Samples

Download data: IDAT, TXT

(Submitter supplied) microRNA sequencing to find differences in miRNA expression in bovine cumulus cells between cumulus pieces retrieved from COCs developing into a blastocyst after fertilization or remain uncleaved after fertilization

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23055

6 Samples

Download data: XLSX