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| Status |
Public on Mar 18, 2008 |
| Title |
Bovine adult versus prepubertal cumulus cells |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
The present study was undertaken to discover molecular markers in bovine cumulus cells predictive of oocyte competence and elucidate their functional significance. Differences in RNA transcript abundance in cumulus cells harvested from oocytes of adult versus prepubertal animals (model of poor oocyte quality) were identified by microarray analysis. Four genes of interest encoding for the lysosomal cysteine proteinases cathepsin B, S, K and Z and displaying greater transcript abundance in cumulus cells surrounding oocytes harvested from prepubertal animals were chosen for further investigation. Greater mRNA abundance for such genes in cumulus cells of prepubertal oocytes was confirmed by real time RT-PCR. Elevated transcript abundance for cathepsins B, S and Z was also observed in cumulus cells surrounding adult metaphase II oocytes that developed to the blastocyst stage at a low percentage following parthenogenetic activation, versus those that developed at a high percentage. Functional significance of cumulus cell cathepsin expression to oocyte competence was confirmed by treatment of cumulus oocyte complexes during in vitro oocyte maturation with a cell permeable cysteine proteinase (cathepsin) inhibitor. Inhibitor treatment decreased apoptotic nuclei in the cumulus layer and enhanced development of parthenogenetically activated and in vitro fertilized adult oocytes to the blastocyst stage. Stimulatory effects of inhibitor treatment during meiotic maturation on subsequent embryonic development were not observed when oocytes were matured in the absence of cumulus cells. Results support a functional role for cumulus cell cathepsins in compromised oocyte competence and suggest that cumulus cell cathepsin mRNA abundance may be predictive of oocyte quality.
Keywords: Bovine, microarray, cDNA, cumulus cells
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| Overall design |
Differences in RNA transcript abundance in cumulus cells harvested from oocytes of adult versus prepubertal animals (model of poor oocyte quality) were identified by microarray analysis.
Total RNA from pools of cumulus cells (n = 4) collected from adult and prepubertal animals for microarray experiments was amplified. Two color microarray experiments were conducted using a bovine cDNA array containing expressed sequence tags (ESTs) representing approximately 15200 unique genes. Hybridizations were performed on duplicate slides (prepubertal versus adult) and incorporated a dye swap. The total number of slides used is eight.
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| Contributor(s) |
Bettegowda A, Patel OV, Lee K, Park K, Ireland JJ, Smith GW |
| Citation(s) |
18417713 |
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| Submission date |
Mar 13, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
George W Smith |
| E-mail(s) |
[email protected]
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| Phone |
517 432 5401
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| Organization name |
Michigan State University
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| Lab |
Laboratoryof Mammalina Reproductive Biology and Genomics
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| Street address |
1230 Anthony Hall
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| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
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| Platforms (1) |
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| Samples (8)
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| GSM272984 |
Bovine adult versus prepubertal cumulus cells, Replicate 1 |
| GSM273013 |
Bovine adult versus prepubertal cumulus cells, Replicate 2 |
| GSM273021 |
Bovine adult versus prepubertal cumulus cells, Replicate 3 |
| GSM273023 |
Bovine adult versus prepubertal cumulus cells, Replicate 4 |
| GSM273052 |
Bovine adult versus prepubertal cumulus cells, Replicate 6 |
| GSM273057 |
Bovine adult versus prepubertal cumulus cells, Replicate 7 |
| GSM273058 |
Bovine adult versus prepubertal cumulus cells, Replicate 8 |
| GSM273059 |
Bovine adult versus prepubertal cumulus cells, Replicate 9 |
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| Relations |
| BioProject |
PRJNA107419 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10819_RAW.tar |
32.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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