GEO DataSets

(Submitter supplied) RNA-seq differential gene expression profiling of Corynebacterium pseudotuberculosis under iron limitation. We use two C. pseudotuberculosis strains, a ciuA Cp13 mutant and its parental wild type T1 strain to quantify the relative gene expression of these strains in cultures with low iron availability. The ciuA Cp13 mutant is deficient for an operon which encodes a iron siderophore uptake system. The Cp13 mutant also showed reduced virulence in CLA infection models. more...

Organism:

Corynebacterium pseudotuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24971

14 Samples

Download data: TXT

(Submitter supplied) The response regulator HrrA belonging to the HrrSA two-component system (previously named CgtSR11) is known to be repressed by the global iron-dependent regulator DtxR in Corynebacterium glutamicum. Sequence analysis indicated an involvement of the HrrSA system in heme-dependent gene expression. Growth experiments revealed that the non-pathogenic soil bacterium C. glutamicum is able to use hemin or hemoglobin as sole iron source. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

9 Samples

Download data: GPR

(Submitter supplied) Background: The human pathogen Corynebacterium diphtheriae is the causative agent of the disease diphtheria. In the 1990s a large diphtheria outbreak in Eastern Europe was caused by the strain C. diphtheriae NCTC 13129. Although the genome was sequenced several years ago, not much is known about the transcriptome. Our aim was to use RNA Sequencing to close this knowledge gap and gain insights into the transcriptional landscape of C. more...

Organism:

Corynebacterium diphtheriae NCTC 13129

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23376

7 Samples

Download data: XLSX

(Submitter supplied) To better understand effects of iron starvation on A. pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. Upon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

4 Samples

Download data: MEV

(Submitter supplied) To better understand effects of iron starvation on A. pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. Upon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

9 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome changes in six serotypes of Actinobacillus pleuropneumoniae in control cultures compared to bacteria grown in media containg the iron chelator 2,2'-dipyridyl.

Organism:

Actinobacillus pleuropneumoniae serovar 5; Actinobacillus pleuropneumoniae serovar 3; Actinobacillus pleuropneumoniae serovar 1 str. 4074; Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 7; Actinobacillus pleuropneumoniae serovar 2 str. 4226; Actinobacillus pleuropneumoniae serovar 6

Type:

Expression profiling by array

Platform:

GPL11003

48 Samples

Download data: XYS

(Submitter supplied) native array description file: 090423_DTU_Actino_xRNA (for array no. 355074A) native array description file: 091013_DTU_Actino_xRNA (for arrays no. 382804A, 382816A, and 431045A) Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Actinobacillus pleuropneumoniae

2 Series

123 Samples

Download data: NDF

(Submitter supplied) In the alpha subclass of proteobacteria iron homeostasis is controlled by diverse iron responsive regulators. Caulobacter crescentus, an important freshwater α-proteobacterium, uses the ferric uptake repressor (Fur) for such purpose. However, the impact of the iron availability on the C. crescentus transcriptome and an overall perspective of the regulatory networks involved remain unknown. In this work we report the identification of iron-responsive and Fur-regulated genes in C. more...

Organism:

Caulobacter vibrioides CB15; Caulobacter vibrioides NA1000

Type:

Expression profiling by array

Platform:

GPL10469

4 Samples

Download data: TXT

(Submitter supplied) Respiration deficient S. aureus small colony variants (SCVs) frequently cause persistent infections, which necessitates they acquire iron, yet how SCVs obtain iron remains unknown. To address this, we created a stable hemB mutant in S. aureus USA300 strain LAC. The hemB SCV utilized exogenously supplied heme but was attenuated for growth under conditions of iron starvation. RNA-seq showed that both WT S. more...

Organism:

Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30628

15 Samples

Download data: XLSX

(Submitter supplied) We assessed changes in gene expression in response to zinc availability for the human pathogen Corynebacterium diphtheriae. Expression profiles of wild-type C. diphtheriae strain 1737 grown in semi-defined metal-poor media (mPGT) without and with 5 µM zinc chloride supplementation were compared; the expression profile of wild-type C. diphtheriae strain 1737 in zinc-replete conditions was also compared against an isogenic Δzur mutant grown in zinc-replete conditions. more...

Organism:

Corynebacterium diphtheriae; Corynebacterium diphtheriae NCTC 13129

Type:

Expression profiling by array

Platform:

GPL25539

9 Samples

Download data: TXT, XLSX

(Submitter supplied) We assessed changes in gene expression in response to manganese availability for the human pathogen Corynebacterium diphtheriae. Total RNA was harvested from wild-type C. diphtheriae strain 1737 and an isogenic ΔmntR (dip0619) grown in semi-defined metal-limited media (mPGT) without or with 5 µM manganese chloride supplementation. Three biological replicates were prepared and five genes were assessed by real-time PCR to validate the array results: dip0124, dip0169, dip0615, dip1923, and dip2261. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL30087

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gluconobacter oxydans; Corynebacterium glutamicum ATCC 13032; Escherichia coli; Corynebacterium glutamicum; Pseudomonas putida; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platforms:

GPL22794 GPL22792

6 Samples

Download data: GPR

(Submitter supplied) Iron is the fourth most abundant element in the Earth’s crust. However, the poor solubility of iron due to oxidation of ferrous iron to the almost insoluble ferric iron under aerobic conditions constitutes a considerable challenge for living organisms to obtain sufficient amounts of the iron available. In the present study, we set out to characterize the global gene expression of C. glutamicum under iron limitation in comparison to iron-replete conditions.

Organism:

Pseudomonas putida; Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL22794

3 Samples

Download data: GPR

(Submitter supplied) Iron is the fourth most abundant element in the Earth’s crust. However, the poor solubility of iron due to oxidation of ferrous iron to the almost insoluble ferric iron under aerobic conditions constitutes a considerable challenge for living organisms to obtain sufficient amounts of the iron available. In the present study, we set out to characterize the global gene expression of C. glutamicum under iron limitation in comparison to iron-replete conditions.

Organism:

Escherichia coli; Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Gluconobacter oxydans; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL22792

3 Samples

Download data: GPR

(Submitter supplied) Exploiting the symbiotic interaction between crops and nitrogen-fixing bacteria is a simple and ecological solution to promote plants growth in prospective extraterrestrial human outposts. In this study, we investigated the adaptation of the legume symbiont Paraburkholderia phymatum to simulated microgravity (s0-g) at the transcriptome level by performing an RNA-Seq analysis. The results revealed a drastic effect on gene expression, with roughly 23 % of P. more...

Organism:

Paraburkholderia phymatum STM815

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22728

6 Samples

Download data: TXT