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| Status |
Public on Sep 06, 2022 |
| Title |
The iron starvation response of the Staphylococcus aureus hemB small colony variant (SCV) |
| Organism |
Staphylococcus aureus subsp. aureus USA300 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Respiration deficient S. aureus small colony variants (SCVs) frequently cause persistent infections, which necessitates they acquire iron, yet how SCVs obtain iron remains unknown. To address this, we created a stable hemB mutant in S. aureus USA300 strain LAC. The hemB SCV utilized exogenously supplied heme but was attenuated for growth under conditions of iron starvation. RNA-seq showed that both WT S. aureus and the hemB mutant sense and respond to iron starvation.
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| Overall design |
A total of 15 samples were analyzed. Samples were acquired from 2 independent experiments, each consisting of 1-3 biological replicates per strain. All samples were grown in iron restricted medium. 4 samples were WT S. aureus treated with ferric ammonium sulfate, 3 samples were WT S. aureus untreated, 3 samples were hemB S. aureus treated with ferric ammonium sulfate, 5 samples were hemB S. aureus untreated.
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| Web link |
https://journals.asm.org/doi/10.1128/JB.00458-21
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| Contributor(s) |
Batko IZ, Guariglia-Oropeza V, Heinrichs DE |
| Citation(s) |
34606375 |
| |
| Submission date |
Sep 14, 2021 |
| Last update date |
Sep 06, 2022 |
| Contact name |
David Heinrichs |
| E-mail(s) |
[email protected]
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| Organization name |
University of Western Ontario
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| Street address |
Microbiology and Immunology, 3014 Dental Sciences Building, 1151 Richmond Street
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| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A5C1 |
| Country |
Canada |
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| Platforms (1) |
| GPL30628 |
NextSeq 550 (Staphylococcus aureus subsp. aureus USA300) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA763127 |
| SRA |
SRP336997 |
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