GEO DataSets

(Submitter supplied) A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

13 Samples

Download data: TXT

(Submitter supplied) Airway epithelial cells and macrophages differ markedly in their responses to influenza A virus (IAV) infection. To investigate transcriptional responses underlying these differences, purified subsets of type II airway epithelial cells (ATII) and alveolar macrophages (AM) recovered from the lungs of mock- or IAV-infected mice were subjected to RNA sequencing. In the absence of infection, AM predominantly expressed genes related to immunity whereas ATII expressed genes consistent with their physiological roles in the lung. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

96 Samples

Download data: TXT

(Submitter supplied) The purpose of this experiment was to understand the pathogenic role of individual 1918 genes on the host response to the 1918 pandemic influenza virus. We examined reassortant avian viruses nearly identical to the pandemic 1918 virus (1918-like avian virus) carrying either the 1918 HA or PB2 gene. Both genes enhanced 1918-like avian virus replication, but only the mammalian host adaptation of the 1918-like avian virus through reassortment of the 1918 PB2 led to increased lethality in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

66 Samples

Download data: TXT

(Submitter supplied) The influenza virus is a major cause of morbidity and mortality worldwide, yet, the impact of intracellular viral invasion and the cellular response diversity remain uncharacterized. By massively parallel single-cell RNA-seq we comprehensively mapped the host lung response to in-vivo influenza infection in wild-type and Irf7-knockout mice across nine immune and non-immune cell types. We found an unexpected high prevalence of infected cells in all cell types, showed that infection is a characteristic property of cell types that is independent of type-I interferon activity, and demonstrated that all cell types responded primarily with a robust generic transcriptional response. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: TXT

(Submitter supplied) Background: Influenza A virus (IAV) infections periodically cause substantial morbidity and mortality in the human population. In the lung, the primary targets for IAV replication are type II alveolar epithelial cells (AECII), which are increasingly recognized for their immunological potential. However, our knowledge of the role of AECII in anti-IAV immunity is incomplete and their in vivo response to infection has not been evaluated. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

28 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

60 Samples

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(Submitter supplied) Respiratory viral infections cause lung epithelial damage, barrier dysfunction and severe disease. Type I interferons (IFN-a/b) are antiviral cytokines whose therapeutic use is limited by well-characterized pleiotropic effects. Type III IFNs (IFN-λ) are less pro-inflammatory and regarded a superior treatment option. Here, we show that IFN signalling reduces lung epithelial proliferation and differentiation and increases epithelial apoptosis during recovery from viral infection. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

45 Samples

Download data: TXT

(Submitter supplied) Respiratory viral infections cause lung epithelial damage, barrier dysfunction and severe disease. Type I interferons (IFN-a/b) are antiviral cytokines whose therapeutic use is limited by well-characterized pleiotropic effects. Type III IFNs (IFN-λ) are less pro-inflammatory and regarded a superior treatment option. Here, we show that IFN signalling reduces lung epithelial proliferation and differentiation and increases epithelial apoptosis during recovery from viral infection. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

15 Samples

Download data: TXT

(Submitter supplied) We employ cDNA microarry(Agilent Mouse lncRNA (4*180K,Design ID:049801) ) to detect the differential expression of mRNAs and lncRNAs in the lung of mice infected with or without A/WSN/33 influenza virus. Microarry analysis revealed that 2092 mRNAs and 1640 lncRNAs differentially expressed in the lung of IAV infected mice compared to control,including 1399 mRNAs and 1052 lncRNAs upregulated, 693 mRNAs and 588 lncRNAs downregulatded. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL21700

6 Samples

Download data: TXT

(Submitter supplied) We used microarrays to compare the gene expression profiles of different H1N1 isolates (seasonal and pandemic) in lung epithelial cells in vitro.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4855

Platform:

GPL570

12 Samples

Download data: CEL

Analysis of well-differentiated primary lung bronchial epithelial cells 36 hs after infection with various H1N1 influenza isolates: seasonal H1N1 BN/59, pandemic H1N1 KY/136 and KY/180. Results provide insight into the molecular basis of host responses to different H1N1 Influenza isolates.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 infection sets

Platform:

GPL570

Series:

GSE48466

12 Samples

Download data: CEL

(Submitter supplied) Array analysis of total lung RNAs from female BALB/c mice collected at 12, 48 and 96 h post-infection with highly and less virulent influenza A (H3N2) viruses. Viruses (designated as LVI and HVI) were derived from influenza strain virus A/Aichi/2/68 (Aichi68). LVI is Aichi68 propagated in eggs, and HVI is mouse adapted Aichi68.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5159

Platform:

GPL6103

18 Samples

Download data: TXT

Analysis of total lung from BALB/c females infected with highly virulent influenza A (HVI) or low virulent influenza A (LVI), up to 96 hrs following infection. Results provide insight into the molecular mechanisms underlying the host pulmonary responses to HVI and LVI infections.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 infection, 3 time sets

Platform:

GPL6103

Series:

GSE55994

18 Samples

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(Submitter supplied) Porcine reproductive and respiratory syndrome virus (PRRSV) remains a serious threat to the swine industry worldwide for many years. PRRSV has a high tropism for swine pulmonary alveolar macrophages (PAM), an essential innate immune cell. Here, we used RNA-sequencing approach to examine the transcriptional profile of in vivo infected and bystander PAM in the lung of PRRSV-infected swine at 7 days post-infection (dpi). more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19176

9 Samples

Download data: TSV

(Submitter supplied) The goals of this study are to compare NGS-derived whole transcriptome profiles (RNA-seq) of H5N1 infected A549 cells overexpressing either negative control mimic or miR-324-5p mimic

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: TXT

(Submitter supplied) We used two different influenza viruses lacking either PB1 or HA to differentiate the antiviral responses generated from primary transcription and full replication, respectively.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL24247 GPL24676

184 Samples

Download data: TXT

(Submitter supplied) Cells respond differently to influenza virus infection after already having been infected previously.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13112 GPL11154

19 Samples

Download data: TXT

(Submitter supplied) We performed RNAseq analysis on primary human airway epithelial cultures either mock infected (PBS) or infected with SARS-CoV-2. Transcriptional profiling studies found that infected pHAE cells had a molecular signature dominated by pro-inflammatory cytokines and chemokine induction, including IL-6, TNFα, CXCL8, and identified NF-κB and ATF4 as key drivers of this pro-inflammatory cytokine response. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: TXT

(Submitter supplied) Eosinophils are terminally differentiated granulocytes that recently have been shown to become infected with Influenza A Virus (IAV). Here, we investigate transcriptional changes that occur to bone marrow-derived eosinophils following exposure to IAV. Global changes in the transcriptome of bone marrow derived mouse eosiniphils were assessed following mock- and Influenza A virus-infection using Affymetrix mouse gene 2.0 ST microarrays.

Organism:

Mus musculus domesticus; Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

20 Samples

Download data: CEL, CHP

(Submitter supplied) Strong differences in the immune response between the two mouse strains were found

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

30 Samples

Download data: TXT