|
| Status |
Public on Aug 29, 2018 |
| Title |
Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.
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| Overall design |
AEC from infected mice were pooled to make three (3) infected GFP+ AEC replicates for sequencing. Five (5) bystander GFP- replicates and five (5) uninfected AEC replicates were also isolated for sequencing
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| Contributor(s) |
Katsikis PD |
| Citation(s) |
30111569, 32678841 |
| |
| Submission date |
Aug 28, 2018 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Peter Katsikis |
| E-mail(s) |
[email protected]
|
| Organization name |
Erasmus MC University Medical
|
| Street address |
Wytemaweg 80
|
| City |
Rotterdam |
| ZIP/Postal code |
3015CN |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA488226 |
| SRA |
SRP158999 |
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