GEO DataSets

(Submitter supplied) We report the gene expression profile of single cell peripheral CD3+ cells from immunodeficient mice injected with human progenitor T-cells (proT-cells). ProT-cell subsets were generated in vitro using human umbilical cord blood-derived CD34+ cells that were either non-expanded (naive), or expanded in vitro with the hydrocarbon receptor antagonist, StemRegenin-1 (SR1).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

2 Samples

Download data: MTX, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

4 Samples

Download data: CSV, MTX, TSV

(Submitter supplied) We report the gene expression profile of single cell peripheral CD3+ cells from immunodeficient mice injected with human progenitor T-cells (proT-cells). ProT-cell subsets were generated in vitro using human umbilical cord blood-derived CD34+ cells that were either non-expanded (naive), or expanded in vitro with the hydrocarbon receptor antagonist, StemRegenin-1 (SR1).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

2 Samples

Download data: CSV

(Submitter supplied) To further development of our gene expression, we have employed whole genome microarray expression profiling. Human cord blood CD34+ cells from healthy donors was cultured for 2 days with or without SL-13R.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

2 Samples

Download data: TXT

(Submitter supplied) mRNA profiling of uncultured and HGFs along with 0.1% DMSO (HGFs) or LY plus Rapa (LR) or SR1-treated hUCB CD34+ cells

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

4 Samples

Download data: TXT

(Submitter supplied) Although practiced clinically for more than 40 years, the use of hematopoietic stem cell (HSC) transplants remains limited by the ability to expand these cells ex vivo. An unbiased screen with primary human HSCs identified a purine derivative, StemRegenin 1 (SR1), that promotes the ex vivo expansion of CD34+ cells. Culture of HSCs with SR1 led to a 50-fold increase in cells expressing CD34 and a 17-fold increase in cells that retain the ability to engraft immunodeficient mice. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3911

Platform:

GPL570

8 Samples

Download data: CEL

Analysis of hematopoietic stem cells (HSC) treated with StemRegenin 1 (SR1) and LGC006 (a less potent SR1 analog) at 30nM to 1000nM. The use of HSC transplants depends on the ability to expand these cells ex vivo. Results provide insight into molecular basis of SR1-induced ex vivo HSC expansion.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 dose sets

Platform:

GPL570

Series:

GSE28359

8 Samples

Download data: CEL

(Submitter supplied) T cells serve as pivotal effectors of the immune system and can be harnessed as therapeutic agents for regenerative medicine and cancer immunotherapy. An unmet challenge in the field is the development of a clinically relevant system that is readily scalable to generate large numbers of T-lineage cells from hematopoietic stem/progenitor cells (HSPCs). Here, we report a stromal cell-free, microbead-based approach that supports the efficient in vitro development of both human progenitor T (proT) cells and mature T cells from CD34+ cells sourced from cord blood, GCSF-mobilized peripheral blood, and pluripotent stem cells (PSCs). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

1 Sample

Download data: MTX, TSV

(Submitter supplied) Expansion for hematopoietic cells from umbilical cord blood is a strategy for use this cell source in clinic transplants, however, it is important to know about the genomic changes that can occur in expanded cells. In order to detect global expression profiles changes in hematopoietic stem and progenitors cells generated in vitro, we analyzed hematopoietics populations obtained by FACS in fresh from umbilical cord blood. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

19 Samples

Download data: CEL, CHP

(Submitter supplied) Multiple myeloma (MM) is a clonal plasma cell disorder frequently accompanied by hematopoietic impairment. Genomic profiling of distinct HSPC subsets revealed a consistent deregulation of signaling cascades, including TGF beta signaling, p38MAPK signaling and pathways involved in cytoskeletal organization, migration, adhesion and cell cycle regulation in MM patients.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

43 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptomic profile of CD34+ cells circulating in peripheral blood of TCIRG1-mutated osteopetrotic patients, compared to cord blood and mobilized CD34+ cells

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

12 Samples

Download data: TXT

(Submitter supplied) We demonstrate that a distinct hematopoietic stem cell gene expression signature was upregulated in human CD34+ cells cultured with Delta1ext-IgG in hypoxia compared to normoxic cultures. Data were submitted on behalf of Dr. Larochelle's lab at the NIH.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

12 Samples

Download data: CSV

(Submitter supplied) We demonstrate that ex vivo culture of human adult hematopoietic stem and progenitor cells with Delta1ext-IgG under hypoxia limits endoplasmic reticulum stress in long-term repopulating hematopoietic stem cells and, to a lesser extent, in lineage committed progenitors compared to normoxic cultures. Data were submitted on behalf of Dr. Larochelle's lab at the NIH.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

4 Samples

Download data: MTX, TSV

(Submitter supplied) The regenerative potential of human hematopoietic stem cells (HSCs) is well established by to their ability of life-long blood cell production and cure of a wide range of hematological diseases upon transplantation. This regenerative potential depends on HSC self-renewal and the coordinated adaptation to metabolic stress conditions. This is especially critical during ex vivo culture/manipulation of HSCs that is frequently accompanied with loss of self-renewal potential resulting in stem cell exhaustion. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

10 Samples

Download data: TAR

(Submitter supplied) Osteolineage cell-derived extracellular vesicles (EVs) play a regulatory role in hematopoiesis and have been shown to promote the ex vivo expansion of human hematopoietic stem and progenitor cells (HSPCs). Here, we demonstrate that EVs from different human osteolineage sources do not have the same HSPC expansion promoting potential. Comparison of stimulatory and non-stimulatory osteolineage EVs by next-generation sequencing and mass spectrometry analyses revealed distinct microRNA (miRNA) and protein signatures identifying EV-derived candidate regulators of ex vivo HSPC expansion. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: TSV

(Submitter supplied) Osteolineage cell-derived extracellular vesicles (EVs) play a regulatory role in hematopoiesis and have been shown to promote the ex vivo expansion of human hematopoietic stem and progenitor cells (HSPCs). Here, we demonstrate that EVs from different human osteolineage sources do not have the same HSPC expansion promoting potential. Comparison of stimulatory and non-stimulatory osteolineage EVs by next-generation sequencing and mass spectrometry analyses revealed distinct microRNA (miRNA) and protein signatures identifying EV-derived candidate regulators of ex vivo HSPC expansion. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15520

12 Samples

Download data: TXT

(Submitter supplied) Hematopoietic stem/progenitor cells (HSPCs) have been successfully used in treating malignancies but with limited cell sources. Whether circulating HSPCs (cHSPCs) in nonmobilized peripheral blood (PB) with engraftment capability could be expanded remains elusive. Here, we developed a polypeptide three-dimensional culture system (3DCS) to expand cHSPCs in nonmobilized PB. We demonstrated that cHSPCs exhibited self-renewal and multilineage differentiation potential by in vitro and in vivo assays including serial transplantation assays. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

8 Samples

Download data: CSV

(Submitter supplied) Purpose: Circulating hematopoietic stem/progenitor cells (cHSPCs) are rare in normal human peripheral blood (PB) without mobilization. We apply a novel three-dimension culture system (3DCS) seeded with no mobilized PB monocytes (PBMNCs), to expand cHSPCs. Two-dimension culture system (2DCS), primary PBMNCs and CD34+ HSPCs derived from bone marrow serves as system, negative and positive control groups respectively. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

13 Samples

Download data: XLS

(Submitter supplied) Human CB HSC enriched cells were transduced with shRNAs targeting MTA1 to identify changes in global transcription programme and understand the mechanisms behind MTA1 knock down induced phenotype.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

9 Samples

Download data: CEL

(Submitter supplied) We compared the transcriptomic changes in hematopoietic stem/progenitor cells (LSK) when Fbxw11 overexpressed. In order to study the function of Fbxw11 in hematopoietic stem/progenitor cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21273

2 Samples

Download data: CSV, H5