GEO DataSets

(Submitter supplied) Background: During the process of elongation, the embryo increases in size within the uterus while the extra-embryonic tissues (EET) develop and differentiate in preparation for implantation. As it grows, the ovoid embryo changes shape into first a tubular and then a filamentous form. This process is directed by numerous genes and pathways, the expression of which may be altered in the case of developmental irregularities such as when the conceptus is shorter than expected or when the embryo develops after splitting. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

24 Samples

Download data: TXT

(Submitter supplied) Objective: To study post-hatching development after transfer of morphologically good quality embryos that survived in vitro maturation under lipolytic conditions using a well-established and recognized bovine model. Design: prospective study with a crossover design. Setting: research laboratory. Animals: 8 healthy multiparous non-lactating Holstein cows used in repeated embryo transfers. Intervention(s): Bovine cumulus oocyte complexes (COCs) were in vitro matured in the presence of either 1) a combination of elevated pathophysiological concentration of palmitic acid (PA) and physiological concentrations of stearic acid (SA) and oleic acid (OA) (HIGH PA), or 2) physiological concentrations of PA, SA and OA (BASAL). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22316

53 Samples

Download data: TXT

(Submitter supplied) Temporal changes in the embryo transcriptome between the blastocyst stage (Day 7) and initiation of elongation (Day 13) differ between in vivo- and in vitro-derived embryos and are reflective of subsequent developmental fate. The aim of this study was to examine the temporal changes in transcriptional profile as the embryo develops from a spherical blastocyst on Day 7 to an ovoid conceptus at the initiation of elongation on Day 13 and to highlight differences in these temporal gene expression dynamics between in vivo- and in vitro-derived blastocysts which may be associated with embryonic survival/mortality using the bovine Affymetrix microarray.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

40 Samples

Download data: TXT

(Submitter supplied) The objective of the present investigation was to utilize the GeneChip® Porcine Genome Array from Affymetrix possessing 20, 201 unique probe sets to identify differentially expressed genes during rapid trophoblastic elongation and attachment to the uterine surface in the pig. Identification and characterization of conceptus gene expression patterns during rapid trophoblastic elongation and attachment in the pig will provide a better understanding of the events required for successful implantation and embryonic survival.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

16 Samples

Download data: CEL

(Submitter supplied) Transcriptome profiling of in vivo-derived porcine conceptus at the tubular stage (12-80 mm) during the elongation period of peri-implantation development. Keywords: Embryo stage of development

Organism:

Sus scrofa

Type:

Expression profiling by SAGE

Platform:

GPL1270

1 Sample

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL18884 GPL15670

6 Samples

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(Submitter supplied) PPARG ChIP seq analysis was conducted to determine genes bound by and potentially regulated by PPARG in the developing ovine conceptus. Determination of gene regulation by prostaglandins through PPARG helps to improve our understanding of early pregnancy events and provides a basis for strategies to improve fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15670

2 Samples

Download data: XLSX

(Submitter supplied) RNA seq analysis was conducted to determine gene expression in the day 14 ovine conceptus. This was used in conjunction with the day 14 PPARG ChIP-seq analysis to identify genes bound by PPARG which were also expressed or not expressed in the day 14 conceptus. Understanding changes in gene expression during early pregnancy is critical to improving fertility and reproductive efficiency in ruminants.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18884

4 Samples

Download data: XLSX

(Submitter supplied) The objective of the present study was to use our own fabricated bovine embryo cDNA microarray to profile genome-wide expression patterns of genes during the implantation period. Duplicate readings were obtained by hybridization of a Cy3/Cy5 probe mixture for each sample. Data normalization was performed the local background intensity of each array spot was smoothed by the local weight regression (lowess) smoother and subtracted from spot intensity data. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS1003

Platform:

GPL1221

11 Samples

Download data: TIFF

Expression profiling of embryos at various days of gestation up to 21 days and fetuses and fetal membranes at 27 and 28 days of gestation. Results provide insight into the regulation of embryonic, fetal, and fetal membrane differentiation.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 7 development stage, 3 tissue sets

Platform:

GPL1221

Series:

GSE1414

11 Samples

Download data: TIFF

(Submitter supplied) Exposure of bovine conceptuses to colony stimulating factor 2 (CSF2) from Day 5 to 7 of development can increase the percent of transferred conceptuses that develop to term. The purpose of this experiment was to understand the mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro in the presence or absence of 10 ng/ml CSF2 from Day 5 to 7 after insemination, transferred into cows, and flushed from the uterus at Day 15 of pregnancy. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

16 Samples

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(Submitter supplied) We report the effect of DKK1 treatment during culture on the length and transcriptome of embryos on day 15 of development, supporting the notion that changes early in development affect later stages of development.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21659

20 Samples

Download data: TXT

(Submitter supplied) A novel porcine cDNA microarray, containing PCR products selected for embryonic expression, was used for transcriptional profiling during conceptus development. Total RNA from quadruplicate samples of small spherical (1-5mm), large spherical (6-11mm), tubular (13-45mm), and filamentous (>100mm) was extracted, amplified, and converted to 32P labeled target and hybridized to membranes. Porcine peri-implantation embryo development order is from small spherical to large spherical, then to Tubular, then to filamentous. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Dataset:

GDS844

Platform:

GPL1209

16 Samples

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Gene expression changes during peri-implantation conceptus development. Small spherical (1-5 mm), large spherical (6-11 mm), tubular (13-45 mm) and filamentous (>100 mm) embryos analyzed.

Organism:

Sus scrofa

Type:

Expression profiling by array, count, 4 development stage sets

Platform:

GPL1209

Series:

GSE1372

16 Samples

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(Submitter supplied) Embryo transfer is largely used in cattle and classically performed at D7 (or D8) using unsorted D7 (or D8) blastocysts produced in vivo, or in vitro in defined media without serum or feeders. Outdated systems including serum and co-culture were however of interest for research purposes. We thus wondered whether embryos that would form a blastocoel at different times after fertilisation (D6 to D8) and stay in culture for up to 2 additional days (D6+1, D6+2, D7+1) would equally develop in vivo after temporary transfer to oestrus-synchronised recipients. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

18 Samples

Download data: TXT

(Submitter supplied) In addition to nourishing the embryo, extra-embryonic tissues (EETs) contribute to early embryonic patterning, primitive hematopoiesis, and fetal health. These tissues are of major importance for human medicine, as well as for efforts to improve livestock efficiency, but they remain incompletely understood. In bovines, EETs are accessible easily, in large amounts, and prior to implantation. We took advantage of this system to describe, in vitro and in vivo, the cell types present in bovine EETs at Day 18 of development. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

14 Samples

Download data: TXT

(Submitter supplied) There is an inevitable need for combining in vitro and in vivo culture systems during specific developmental time points to facilitate a comprehensive understanding of early embryo development which would yield insights into the molecular pathways controlling early development and to improve our knowledge in regulation of embryonic development. In current study, we aimed to understand the influences of in vitro culture conditions on bovine blastocyst originated in vivo at 2- or 8-cell stagre. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: TXT

(Submitter supplied) Axis specification in mouse is determined by a sequence of reciprocal interactions between embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as ‘patterning’ the embryo. Considering these interactions as essential, but lacking in most mammals the genetically driven approaches used in mouse and the corresponding patterning mutants, we examined whether a molecular signature originating from extra-embryonic tissues could relate to the developmental stage of the embryo proper and predict it. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

20 Samples

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