GEO DataSets

(Submitter supplied) RNA-seq was performed by infecting primary mammary epithelial cells (pMECs) with both gram negative (E.coli) and gram positive bacteria (S. aureus). Using stringent pipeline, a set of 1957 known and 1175 novel lncRNAs were identified, among which, 112 lncRNAs were found differentially expressed in bacteria challenged PMECs compared with the control.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19149

7 Samples

Download data: TXT

(Submitter supplied) Five healthy Laoshan dairy goats (four years old, third lactation) from Qingdao Laoshan dairy goat primary farm (Shandong Province, China) were used. The mammary gland samples were collected surgically after general anaesthesia using Xylazine Hydrochloride injection solution (Huamu Animal Health Products Co., Ltd. China) at corresponding lactation stage, including early, peak and late lactations.

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15473

3 Samples

Download data: TXT

(Submitter supplied) Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to explore the lncRNA profile on mastitis.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

9 Samples

Download data: XLSX

(Submitter supplied) Mycoplasma bovis (M. bovis) causes to the diseases such as arthritis, pneumonia, abortion and mastitis causing to great losses in dairy bovine industries. It undertakes significant functions in the regulation of the immune response formed by many RNA type bacteria such as Messenger RNA (mRNAs) and Long noncoding RNA (lncRNAs). mRNA and lncRNA expression profiles still cannot be majorly known in the bovine mammary gland tissues infected with M. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

5 Samples

Download data: GTF, XLSX

(Submitter supplied) Purpose: The goal of this study is to explore the role of miRNAs in dairy cow response to E. coli and S. aureus, mastitis causing pathogens, is not well understood. Results: The global expression of miRNAs in bovine mammary epithelial cells (MAC-T cells) challenged with heat-inactivated Staphylococcus aureus (S. aureus) or Escherichia coli (E. coli) bacteria (treatments: 6, 12, 24 and 48 hr) and without challenge (control: 0, 6, 12, 24 and 48 hr) was profiled using next-generation-sequencing. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17875

13 Samples

Download data: TXT

(Submitter supplied) MicroRNA expression patterns indicating immune competence of PMEC

Organism:

Sus scrofa; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL16384

9 Samples

Download data: CEL

(Submitter supplied) Porcine circovirus type 2 (PCV2) can cause severe disease in the affected pigs, resulting in massive economic loss for the swine industry. Transcriptomics and proteomics approaches have been widely employed to identify the underlying molecular mechanisms of PCV2 infection. Numerous differentially expressed mRNAs, miRNAs, and proteins, together with their associated signaling pathways, have been identified, paving the way to further validation of their biological functions. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22918

6 Samples

Download data: XLS

(Submitter supplied) Establishment of an in vitro system to explore molecular mechanisms of mastitis susceptibility in cattle by comparative expression profiling of Escherichia coli and Staphylococcus aureus inoculated primary cells sampled from cows with different genetic predisposition for somatic cell score Primary bovine mammary gland epithelial cells (pbMEC) were sampled from the udder parenchyma of cows that were selected for high and low mastitis susceptibility by applying a marker assisted selection strategy considering QTL and molecular marker information of a repetitively confirmed QTL for SCS in the telomeric region on BTA18 The cells were cultivated and subsequently inoculated with heat inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4437

Platform:

GPL2112

88 Samples

Download data: CEL

Mammary gland epithelial cells of cows with favorable somatic cell score (SCS) QTL allele Q and unfavorable allele q after S. aureus or E.coli exposure for up to 24 hours. SCS is an indicator of milk quality and udder health. Results provide insight into molecular basis of mastitis susceptibility.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL2112

Series:

GSE24560

88 Samples

Download data: CEL

(Submitter supplied) We applied RNA sequencing analysis to 6 hypothalamuses from pubertal and prepubertal goats. The significant differential expression transcripts were screened out. In order to validate the RNA-seq data, the expression patterns in the samples of lncRNAs and mRNA genes were confirmed by qRT-PCR. This research provides an expression profile of lncRNAs to further research on puberty.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22146

6 Samples

Download data: TXT

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

36 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21299

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:

GPL27106 GPL21299 GPL25639

46 Samples

Download data: TXT

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL25639

2 Samples

Download data: TXT

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

11 Samples

Download data: TXT

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

23 Samples

Download data: TXT, XLS

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27106

6 Samples

Download data: TXT