GEO DataSets

(Submitter supplied) Transcriptomic analyses using high-throughput methods have revealed abundant antisense transcription in bacteria. Most frequently, antisense transcription is due to the overlap of mRNAs with long 5’ regions or 3’ ends that extend beyond the coding sequence. In addition, antisense RNAs that do not contain any coding sequence are also observed. Nostoc sp. PCC 7120 is a filamentous cyanobacterium that, under nitrogen limitation, behaves as a multicellular organism with division of labor among two different cell types that depend on each other, the vegetative CO2-fixing cells and the nitrogen-fixing heterocysts. more...

Organism:

Nostoc sp. PCC 7120 = FACHB-418

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29232

6 Samples

Download data: GFF, TXT

(Submitter supplied) Here, we report the transcriptome of Anabaena sp. strain 7120, a cyanobacterium that forms specialized nitrogen-fixing cells called heterocysts. Our data suggests that cyanobacteria frequently have more complex transcripts than thought, with large 5' UTRs, numerous antisense transcripts, and multiple transcriptional start sites or processing sites.

Organism:

Nostoc sp. PCC 7120 = FACHB-418

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11645

4 Samples

Download data: BAM

(Submitter supplied) In order to identify possible RNAs regulated by small RNA NsiR3 we have analyzed the responses to nitrogen removal in a mutant lacking nsiR3 (ΔnsiR3). RNA samples were isolated from cells growing in the presence of combined nitrogen (ammonium) or after 8 h of nitrogen deprivation.

Organism:

Nostoc sp. PCC 7120 = FACHB-418

Type:

Expression profiling by array

Platform:

GPL25601

4 Samples

Download data: TXT

(Submitter supplied) In order to analyze the responses of a heterocystous cyanobacterium to combined-nitrogen depletion/addition, we have used customized microarrays to identify genes potentially involved in heterocysts differentiation by comparing the responses in the control strain and a hetR mutant unable to differentiate heterocysts. RNA samples were isolated from cells growing in the presence of combined nitrogen (ammonium) or after 6, 8, 12 or 24 h of nitrogen deprivation. more...

Organism:

Nostoc sp. PCC 7120 = FACHB-418

Type:

Expression profiling by array

Platform:

GPL25601

20 Samples

Download data: TXT

(Submitter supplied) We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences.

Organism:

Nostoc sp. PCC 7120 = FACHB-418

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17863

2 Samples

Download data: TXT

(Submitter supplied) The CRP-family transcription factor NtcA, universally found in cyanobacteria, was initially discovered as a regulator operating N control. It responds to the N regime signaled by the internal 2-oxoglutarate levels, an indicator of the C to N balance of the cells. NtcA-activated canonical promoters bear an NtcA-consensus binding site (GTAN8TAC) centered at about 41.5 nucleotides upstream from the transcription start point. more...

Organism:

Anabaena

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17858

2 Samples

Download data: BED, TXT