GEO DataSets

(Submitter supplied) Hidradenitis suppurativa (HS) is an inflammatory skin disease with limited therapeutic options. We and others have previously identified an abnormal B cell infiltrate within HS lesional skin. We performed scRNASequencing on CD3 negative cells from inflammatory HS skin lesions, healthy control skin and matched blood to better understand infiltrating B cells amongst other immune cells within lesional skin.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

20 Samples

Download data: MTX, TSV

(Submitter supplied) Hidradenitis suppurativa (HS) skin lesions are infiltrated by numerous inflammatory cell types, which may be subject to and can act upon surrounding tissue stroma and epithelium. Understanding how these networks are locally organized can help identify key nodes sustaining inflammatory and fibrotic processes.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL18573

3 Samples

Download data: CSV, JPG, JSON, MTX, PNG, TSV

(Submitter supplied) Hidradenitis suppurativa (HS) is an inflammatory skin disease with limited therapeutic options. CD4 T Cells have been described as more inflammatory than T cells in healthy skin. To better understand alterations within the T cell compartment, we profiled CD4 Teffector cells and regulatory T cells (Treg) from inflammatory HS skin lesions and healthy control skin via scRNASequencing.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

14 Samples

Download data: MTX, TSV

(Submitter supplied) Hidradenitis suppurativa (HS) is an inflammatory skin disease with limited therapeutic options. Identifying transcriptional alterations within non-immune populations of HS lesions versus healthy controls could identify novel targets for therapy. We profiled subsets sorted as live, CD45 negative cells from biopsies of inflammatory lesions of three patients with HS and six specimens from healthy controls to determine what cell types and pathways were altered in HS inflammatory lesions.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

9 Samples

Download data: MTX, TSV

(Submitter supplied) To increase disease understanding and investigate cell types and pathways active in Hidradenitis suppurativa, skin was collected from patients who had surgical removal of affected skin regions. Blocks of tissue were taken from regions defined as lesional and non-lesional. For comparison blocks of skin were also collected from healty people undergoing plastic surgery

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

33 Samples

Download data: CEL

(Submitter supplied) Hidradenitis Suppurativa (HS) is a debilitating chronic inflammatory disease characterized by chronic abscess formation and development of multiple draining sinus tracts in the groin, axillae, and perineum. Through the use of transcriptomic approaches, we characterize the inflammatory responses in HS in depth, revealing the cell types and immune responses involved.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

9 Samples

Download data: MTX, TSV

(Submitter supplied) Hidradenitis Suppurativa (HS) is a debilitating chronic inflammatory disease characterized by chronic abscess formation and development of multiple draining sinus tracts in the groin, axillae, and perineum. Through the use of transcriptomic approaches, we characterize the inflammatory responses in HS in depth, revealing the cell types and immune responses involved.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

63 Samples

Download data: TXT

(Submitter supplied) Hidradenitis suppurativa (HS) is a highly prevalent, morbid inflammatory skin disease with limited treatment options. The major cell types and inflammatory pathways in skin of HS patients are poorly understood. We profiled via scRNASeq myeloid cells and keratinocytes sort-purified from two healthy skin samples and two samples with HS pathology to determine major cell types and transcriptional pathways altered in HS.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

8 Samples

Download data: MTX, TSV

(Submitter supplied) NOT PROVIDED; REQUESTED

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

87 Samples

Download data: CSV

(Submitter supplied) The immunopathogenesis of psoriasis, a common chronic inflammatory disease of the skin, is still incompletely understood. Here we demonstrate, using a combination of single-cell and spatial-sequencing, that psoriatic fibroblasts, a hitherto unappreciated participant in psoriasis pathogenesis, contribute to the immune network in psoriasis through transition to a pro-inflammatory state characterized by CCL19 and CCL13. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

33 Samples

Download data: CSV

(Submitter supplied) Treatment for the debilitating, chronic skin disease hidradenitis suppurativa (HS) is grossly inadequate. A better understanding of its pathogenesis is required to design new therapeutic strategies. Despite an incidence of approximately 1 in 200, HS is underrecognized and underdiagnosed, with a high morbidity and poor quality of life, which may last decades. In this study we first employed single cell RNA sequencing to analyse gene expression in immune cells isolated from HS lesions compared with healthy control skin. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

9 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) Objective: To better understand the immune microenvironment of pancreatic ductal adenocarcinomas (PDACs), here we explored the relevance of T and B cell compartmentalization into tertiary lymphoid structures (TLSs) for the generation of local antitumor immunity. Design: We characterized the functional states and spatial organization of PDAC-infiltrating T and B cells using single-cell RNA sequencing (scRNA-seq), flow cytometry, multicolor immunofluorescence, gene expression profiling of microdissected TLSs, as well as in vitro assays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL33019

19 Samples

Download data: RCC

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

36 Samples

Download data: BIGWIG, MTX, NARROWPEAK, TSV

(Submitter supplied) In this study, we used single-cell RNA-sequencing to reveal how cellular heterogeneity of Hidradenitis Suppurativa (HS) lesional epidermis was reshaped at the transcriptional level. By comparison with healthy interfollicular epidermal basal cells, in HS we revealed marked gene signatures centered on mitotic chromosome segregation, DNA replication and repair as well as cell-cell adhesion and chromatic remodeling. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

4 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) By using dual-omics profiling for both chromatin accessibility and gene expression as well as CUT&RUN sequencing for chromatin modification states, we identified that numerous disease-associated inflammatory enhancers were epigenetically activated and co-exist with the transcriptional states of their target genes in basal progenitor cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: TXT

(Submitter supplied) 1) By using dual-omics profiling for both chromatin accessibility and gene expression as well as CUT&RUN sequencing for chromatin modification states, we identified that numerous disease-associated inflammatory enhancers were epigenetically activated and co-exist with the transcriptional states of their target genes in basal progenitor cells. 2) By using CUT&RUN sequencing , we identified numerous S100A8 binding DNA sequences in HS epidermal cells.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

20 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) By using dual-omics profiling for both chromatin accessibility and gene expression as well as CUT&RUN sequencing for chromatin modification states, we identified that numerous disease-associated inflammatory enhancers were epigenetically activated and co-exist with the transcriptional states of their target genes in basal progenitor cells.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) Hidradenitis Suppurativa molecular taxonomy and key signaling pathways were studied by whole transcriptome profiling. Dysregulated genes were detected by comparing lesional and non lesional skin obtained from female HS patients and matched healthy controls using the Agilent array platform

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16699

24 Samples

Download data: TXT

(Submitter supplied) This study investigated the underlying inflammatory pathways and cell types in hidradenitis suppurativa using transcriptomic approaches with RNA sequencing of lesional and non-lesional skin biopsies from hidradenitis suppurativa patients.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

30 Samples

Download data: XLSX