GEO DataSets

Analysis of splenocytes from animals infected with virB mutant Brucella arbortus strains. The virB operon encodes a Type IV secretion system (T4SS) essential for the intracellular survival and persistent infection by Brucella species. Results provide insight into the role of T4SS in infections.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 genotype/variation, 3 infection sets

Platform:

GPL1261

Series:

GSE6810

10 Samples

Download data: CEL

(Submitter supplied) The virB operon, encoding a Type IV secretion system (T4SS), is essential for intracellular survival and persistent infection of Brucella spp. To better understand the role of the T4SS in evading host defense mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild type Brucella strains and strains that fail to express the virB genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2859

Platform:

GPL1261

10 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platforms:

GPL81 GPL32

16 Samples

Download data: CEL, CHP

(Submitter supplied) Brucella dynamically engage macrophages while trafficking to an intracellular replicative niche as macrophages, the first line of innate host defense, attempt to eliminate organisms. Brucella melitensis, B. neotomae, and B. ovis are highly homologous, yet exhibit a range of host pathogenicity and specificity. RAW 264.7 macrophages infected with B. melitensis, and B. ovis exhibit divergent patterns of bacterial persistence and clearance; conversely, B. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

11 Samples

Download data: CEL, CHP

(Submitter supplied) A mini-microarray containing spotted oligos corresponding to 289 B. melitensis genes was used to identify candidate genes whose transcription is influenced by BlxR. This microarray contains genes encoding the type IV secretion system, flagella, transcriptional regulators, transporters, and proteins involved in outer membrane biogenesis, iron acquisition, and denitrification. RNA was prepared from both B. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6170

14 Samples

Download data

(Submitter supplied) A whole genome expression comparision between wild type B. melitensis 16M and ΔBM-LOV-HK (ΔBMEII0679) B. melitensis strains grown in broth culture to determine genes directly and indirectly regulated by BM-LOV-HK.

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL16337

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) In a time course study, we characterized global gene expression profile of B. melitensis-infected bovine Peyer patches in the first 4 h p.i. Microarray analysis revealed that 2,916 bovine genes were detected as differentially expressed (z-score p < 0.025) in loops inoculated with virulent B. melitensis 16M compared with controls between 15 min and 4 h post-infection. From these genes, 2,286 (78%) were up- and 630 (22%) were down-regulated. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

20 Samples

Download data: GPR

(Submitter supplied) Facultative intracellular Brucella infect and survive inside macrophages, and the outcome of macrophage-Brucella interaction is a basis for establishment of a chronic Brucella infection. The majority of Brucella are killed at the early infection stage. A subpopulation of virulent Brucella strains is instead trafficked to an intracellular replicative phagosome, and are resistant to further attack and begin to multiply dramatically. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Brucellosis is still a widespread zoonotic disease. Very little is known about the interaction between B. abortus and trophoblastic cells, which is essential for better understanding the pathogenesis of the Brucella-induced placentitis and abortion, a key event for transmission of the disease. The goal of this study was to evaluate the profile of gene expression by bovine trophoblastic cells during infection with B abortus. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

3 Samples

Download data: TXT

(Submitter supplied) In order to uncover transcriptional changes occurring in Brucella- infected mouse brains that might lead to neurologic complications, we infected susceptible mice intranasally with Brucella and harvested their brains.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

10 Samples

Download data: XLSX

(Submitter supplied) Brucella spp. is an intracellular pathogen in vivo. The intracellular B. melitensis transcriptome was determined by initially enriched and then amplified B. melitensis RNA from total RNA of B. melitensis-infected HeLa cells. Analysis of microarray results identified 161 and 115 genes differentially expressed at 4 and 12 h p.i., respectively. Most of the genes (78%) differentially expressed were down-regulated at the earliest time point, but up-regulated (75%) at 12 h p.i. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL8156

18 Samples

Download data: GPR

(Submitter supplied) In a time course study, we characterized global gene expression profile of B. melitensis-infected epithelium-like cells at the onset of infection. B. melitensis-infected HeLa cells exhibited a down-regulated expression profile at 4 h (48 up- and 109 down-regulated genes) that transitioned to an activated transcriptional profile at 12 h post-infection (733 up- and 224 down-regulated genes). The analysis of the results indicates that infected cells undergo an adaptation period during the first 4 h p.i. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8155

16 Samples

Download data: GPR