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Platform GPL2853 Query DataSets for GPL2853
Status Public on Oct 14, 2005
Title UIUC Bos taurus 13.2K 70-mer oligoarray
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Bos taurus
Manufacturer W.M. Keck Center, University of Illinois Urbana-Champaign
Manufacture protocol 70-mer oligos were printed in 150 mM Phosphate buffer at 20 uM concentration.
Support glass
Coating aminosilane
 
Description The 13,257 oligos used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries and based upon the earlier cDNA array platform GPL2864 (Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245) and subtracted cDNA libraries created from embryonic (day 36 and day 64) and extra-embryonic (day 14 to 25) tissues (NCBI libraries 15993,15993, and 17188). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All 70-mer oligos were printed in duplicate on the array. Keywords: oligo, cow, placenta, bovine, embryo, microarray
 
Web link http://cagst.animal.uiuc.edu/microarray/
Contributor(s) Everts RE, Hue I, Sandra O, Renard J, Tian XC, Yang X, Lewin HA
Submission date Sep 16, 2005
Last update date Jun 03, 2011
Contact name Harris A Lewin
Phone 217-244-6745
Fax 217-244-5617
URL http://cagst.animal.uiuc.edu/Lewin.html
Organization name University of Illinois
Department Animal Sciences
Lab Laboratory of Immunogenetics
Street address 1201 West Gregory Drive
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Samples (623) GSM97322, GSM97323, GSM97325, GSM97326, GSM97327, GSM97328 
Series (14)
GSE4304 Nutrition-induced ketosis alters metabolic and signaling gene networks in liver of periparturient dairy cows
GSE8923 Aberrant gene expression patterns in placentomes of cattle cloned by somatic cell nuclear transfer
GSE9162 Major differences in gene expression profiles revealed in day-25 trophoblast but not embryonic discs from cattle clones
Relations
Alternative to GPL13515
Alternative to GPL8776
Alternative to GPL9689

Data table header descriptions
ID
Block Block on the array
Row row within block
Column column within block
GB_ACC GenBank accession number when available
SPOT_ID Local spot identifier
SEQUENCE

Data table
ID Block Row Column GB_ACC SPOT_ID SEQUENCE
1 1 1 1 AI495218 OLIGO_00024 CAACGGCTTCATCCGTATCATCGGATTCGACAACGTTCGCCAAGTGCAGTGCATCAGCTTCATCGCCTAC
2 1 1 2 AI495218 OLIGO_00024 CAACGGCTTCATCCGTATCATCGGATTCGACAACGTTCGCCAAGTGCAGTGCATCAGCTTCATCGCCTAC
3 1 1 3 BUFFER
4 1 1 4 BUFFER
5 1 1 5 DR697717 OLIGO_03948 TGGGTGGCCTCGCCCTCAAGGGGCTTGCACGGGGCCACGAGAACGGGCCTGGAACTCCTCCATGTACCTG
6 1 1 6 DR697717 OLIGO_03948 TGGGTGGCCTCGCCCTCAAGGGGCTTGCACGGGGCCACGAGAACGGGCCTGGAACTCCTCCATGTACCTG
7 1 1 7 BF045945 OLIGO_04044 CTGGTGAAATATGATGGAATTGACTGTGTCTATGGACTGGAACTTCACAGAGATAAAAGGATTTTAAAGC
8 1 1 8 BF045945 OLIGO_04044 CTGGTGAAATATGATGGAATTGACTGTGTCTATGGACTGGAACTTCACAGAGATAAAAGGATTTTAAAGC
9 1 1 9 AW464959 OLIGO_03839 GGCATACTTTTTAGAAATCGTTTTGGGGATGATTAAGGCCGAGTAAAAATTTATGAATTCCTTGCCTTGT
10 1 1 10 AW464959 OLIGO_03839 GGCATACTTTTTAGAAATCGTTTTGGGGATGATTAAGGCCGAGTAAAAATTTATGAATTCCTTGCCTTGT
11 1 1 11 AW465775 OLIGO_03887 TTGACTTTGTTGCCAAAAACAAGCCCATCAGGACCTTCTCCCCCTTCTTCCCATTTCTCCTTCCCACTTC
12 1 1 12 AW465775 OLIGO_03887 TTGACTTTGTTGCCAAAAACAAGCCCATCAGGACCTTCTCCCCCTTCTTCCCATTTCTCCTTCCCACTTC
13 1 1 13 DR697701 OLIGO_03935 AAGCAGTGAGAATACATGTTTTTTTCCACATGTGTGGCCTGAATTTCTCCCAAATTTAGACTATGCCAAA
14 1 1 14 DR697701 OLIGO_03935 AAGCAGTGAGAATACATGTTTTTTTCCACATGTGTGGCCTGAATTTCTCCCAAATTTAGACTATGCCAAA
15 1 1 15 BF042443 OLIGO_04031 CAGCATGTAGCTATTCCTCTTACCCTTCTAATGCAGTCTGTCTTGGTCTCTACAGAGCTTCAGTCTCACC
16 1 1 16 BF042443 OLIGO_04031 CAGCATGTAGCTATTCCTCTTACCCTTCTAATGCAGTCTGTCTTGGTCTCTACAGAGCTTCAGTCTCACC
17 1 1 17 CR454453 OLIGO_07969 GACACATTACTAATACTGTAGGAATTGGTGAGGCCTTGACTTAAAAACTTTCTTTGTACTGTGATTTCCT
18 1 1 18 CR454453 OLIGO_07969 GACACATTACTAATACTGTAGGAATTGGTGAGGCCTTGACTTAAAAACTTTCTTTGTACTGTGATTTCCT
19 1 1 19 CN439999 OLIGO_08064 TAGTTCGAGCCCTTATGACAACTGTCTGCTATTCTGCAATTATCTTTGAGTCTTCTCTCCGAGTGGATGT
20 1 1 20 CN439999 OLIGO_08064 TAGTTCGAGCCCTTATGACAACTGTCTGCTATTCTGCAATTATCTTTGAGTCTTCTCTCCGAGTGGATGT

Total number of rows: 27648

Table truncated, full table size 2768 Kbytes.






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