GEO DataSets

Analysis of cervix and skin from 6 week-old, virgin, transgenic females expressing HPV16 E6 oncoprotein. K14E6 transgenics present a more aggressive phenotype in the skin than in the cervix. Results provide insight into the molecular basis for this observed difference in E6 effect.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 tissue sets

Platform:

GPL8321

Series:

GSE10702

8 Samples

Download data: CEL, CHP

(Submitter supplied) Background The in vivo properties of HR-HPV E6 and E7 oncoproteins have been previously evaluated through the generation and characterization of HPV transgenic mouse strains. Although K14E6 transgenic mice develop spontaneous tumors of the skin epithelium, no spontaneous reproductive tract malignancies arise, unless the transgenic mice were treated chronically with 17β-estradiol. Taken together, these findings suggest that E6 performs critical functions in normal adult cervix and skin, highlighting the need to define E6-controlled transcriptional programs in these tissues. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3530

Platform:

GPL8321

8 Samples

Download data: CEL, CHP

(Submitter supplied) We analyzed the effects of the expression of HPV 16 E6 oncoprotein variants (AA-a, AA-c, E-A176/G350, E-C188/G350, E-G350), and the E- Prototype in global gene expression profiles in an in vitro model. E6 gene was cloned into an expression vector fused to GFP and was transfected in C33-A cells. Affymetrix GeneChip Human Transcriptome Array 2.0 platform was used to analyze the expression of over 245,000 coding transcripts. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

16 Samples

Download data: CEL, CHP

(Submitter supplied) We performed microarray analysis of the effect of productive HPV16 infection in cervical, foreskin and tonsil epithelial trancriptome. Our study for the first time compares and contrast the gene expression changes and differential activation of cellular pathways induced by the virus in the three tissue types.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

30 Samples

Download data: TXT

(Submitter supplied) Global long non-coding RNA (lncRNA) expression profile in primary human keratinocytes post-expression of high-risk HPV-16 E6 oncoprotein Purpose: The goal of this study is to utlize high-throughput RNA sequencing (RNA-seq) to identify differentially expressed host lncRNAs with the presence of the high-risk HPV-16 E6 oncoprotein in primary human foreskin keratinocytes. Methods: Primary human foreskin keratinocytes (HEKa) stably expressing HPV-16 E6 compared to GFP control were analyzed, in triplicates, by RNA-seq (Illumina). more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: TXT

(Submitter supplied) The HPV16-E7 oncoprotein and 17β-estradiol are import factors for the induction of premalignant lesions and cervical cancer. The study of these factors is crucial for a better understanding of cervical tumorigenesis. In this study, we performed a microarray analysis to obtain a global gene expression profile induced by both, HPV16-E7 and 17β-estradiol in cervical tissue of K14E7 transgenic mice. We found that 17β-estradiol is the main cause of the up-regulation of a large number of cellular genes involved in the immune response whereas E7 oncoprotein mainly affects the cellular metabolism. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

15 Samples

Download data: CEL

(Submitter supplied) The life cycle of human papillomaviruses (HPV) is strictly linked to the differentiation of their natural host cells. The HPV E6 and E7 oncoproteins can delay the normal differentiation program of keratinocytes, however, the exact mechanisms responsible for this have not yet been identified. The goal of this study was to investigate the effects of HPV16 oncoproteins on the expression of genes involved in keratinocyte differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL, CHP

(Submitter supplied) Background NASP (Nuclear Autoantigenic Sperm Protein) is a linker histone chaperone required for normal cell division. Changes in NASP expression significantly affect cell growth and development; loss of gene function results in embryonic lethality. However, the mechanism by which NASP exerts its effects in the cell cycle is not understood. To understand the pathways and networks that may involve NASP function, we evaluated gene expression in HeLa cells in which NASP was either overexpressed or inhibited by siRNA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

8 Samples

Download data: TXT

(Submitter supplied) Background: Lung cancer is a multistage process with poor prognosis and high morbidity. Importantly, the genetics of dysplasia, a facultative cancer, at the edge of malignant transformation is unknown. Results: We employed laser microdissection to harvest dysplastic as opposed to transgenic but otherwise morphologically unaltered epithelium and compared findings to non-transgenic lung. We then employed microarrays to search genome wide for gene regulatory networks. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3825

Platform:

GPL1261

15 Samples

Download data: CEL, CHP

Analysis of dysplastic lung cells and transgenic but morphologically unaltered lung cells, both harvested from SP-C/c-raf transgenics. Normal lung from non-transgenic littermates also examined. Results provide insight into molecular mechanisms underlying an early stage of lung cancer development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age, 3 disease state, 2 gender, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE13963

15 Samples

Download data: CEL, CHP