GEO DataSets

Analysis of cyanobacterium cultures subjected to zinc excess (776 uM) for up to 4 hrs. Although an essential metal, Zn at excess levels can disturb metal homeostasis leading to oxidative stress. Results provide insight into molecular mechanisms underlying cyanobacteria stress response to Zn toxicity

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL3214

Series:

GSE3755

4 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Datasets:

GDS3739 GDS3740 GDS3741 GDS3742 GDS3743

Platforms:

GPL3214 GPL3095

44 Samples

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(Submitter supplied) The transcriptome approach was used to characterize the global change in slr1738 null-mutant gene expression in response to cadmium (50 microM, 180 min), compared to wild type strain gene expression in response to cadmium (50 microM, 180 min). Total RNA were isolated from null-mutant and wild type stressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

2 Samples

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(Submitter supplied) The transcriptome approach was used to characterize the of the global change in Synechocystis gene expression in response to continuous exposure to cadmium (50 microM). Total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

2 Samples

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(Submitter supplied) The transcriptome approach was used to characterize the global change (2 Fe concentrations) in Synechocystis gene expression in response to continuous iron starvation, compared to standard medium (17 microM). For iron depletion, exponentially growing cells were washed in Fe-free medium, and grown for 48 hrs under standard conditions in liquid medium cotaining either 1 mM or 2 mM of ferric ammonium citrate. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

4 Samples

Download data

(Submitter supplied) The transcriptome approach was used to characterize the of the global change (2 time points) in Synechocystis gene expression in response to continuous exposure to zinc excess (776 microM), compared to standard medium (776 nM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

4 Samples

Download data

(Submitter supplied) The transcriptome approach was used to characterize of the global change (2 time points) in Synechocystis gene expression in response to continuous exposure to iron excess (17 mM), compared to standard medium (17 microM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

4 Samples

Download data

(Submitter supplied) The transcriptome approach was used to characterize the kinetics (5 time points) of the global change in Synechocystis gene expression in response to continuous exposure to H2O2 (3 mM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3214

10 Samples

Download data

(Submitter supplied) The transcriptome approach was used to characterize the kinetics (9 time points) of the global change in Synechocystis gene expression in response to continuous exposure to Cd (CdSO4, 50 _M). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point), covering 2,845 of the 3,168 ORFs of Synechocystis (Custom-commercial array : IntelliGeneTM CyanoCHIP version 1.2, TAKARA). more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL3095

18 Samples

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Analysis of cyanobacterium cultures subjected to iron starvation. Fe concentrations started at 1uM or 2uM, and ended at 0.5uM. Fe is required for the growth and development of all organisms. Results provide insight into molecular mechanisms underlying cyanobacteria stress response to Fe limitation.

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array, log2 ratio, 2 dose sets

Platform:

GPL3214

Series:

GSE3755

4 Samples

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Analysis of cyanobacterium cultures subjected to iron excess (17 mM) for up to 6 hrs. Although an essential metal, Fe at excess levels can disturb metal homeostasis leading to oxidative stress. Results provide insight into molecular mechanisms underlying cyanobacteria stress response to Fe toxicity.

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL3214

Series:

GSE3755

4 Samples

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Analysis of cyanobacterium cultures subjected to hydrogen peroxide excess (3 mM) for up to 7 hrs. H2O2 is the paradigm reactive oxygen species (ROS) agent. Results provide insight into molecular mechanisms underlying cyanobacteria stress response to H2O2 toxicity.

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array, log2 ratio, 5 time sets

Platform:

GPL3214

Series:

GSE3755

10 Samples

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Analysis of cyanobacterium cultures subjected to cadmium excess (50 uM) for up to 16 hrs. Cd, a toxic metal, is very abundant in the environment. Results provide insight into molecular mechanisms underlying cyanobacteria stress response to Cd toxicity.

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array, log2 ratio, 9 time sets

Platform:

GPL3095

Series:

GSE3755

18 Samples

Download data

(Submitter supplied) Iron is an essential cofactor in many metabolic reactions. Mechanisms controlling iron homeostasis need to respond to changes in extracellular conditions, but must also keep the concentration of intracellular iron under strict control, as free ferrous iron (Fe2+) can lead to the generation of reactive oxygen species. Due to its role as redox carrier in photosynthesis, the iron quota in cyanobacteria is about 10 times higher than in model enterobacteria, but the molecular details how such high quota is tightly regulated have remained obscure. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL15867

12 Samples

Download data: TXT

(Submitter supplied) Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO4) and that cells exposed to 1mM Cd exhibit a cellular Cd concentration of 67µM. more...

Organism:

Thermococcus gammatolerans

Type:

Expression profiling by array

Platform:

GPL6958

24 Samples

Download data: GPR

(Submitter supplied) Arsenic is an ubiquitous contaminant and a toxic metalloid which presents two main redox states in nature: arsenite [AsIII] and arsenate [AsV]. Arsenic resistance in Synechocystis sp. strain PCC 6803 is mediated by the arsBHC operon and two additional arsenate reductases encoded by the arsI1 and arsI2 genes. Here we describe the genome-wide responses in response to the presence of arsenate and arsenite in wild type and in mutants in the arsenic resistance system. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL17790

28 Samples

Download data: TXT

(Submitter supplied) Iron and manganese are part of a small group of transition metals required for photosynthetic electron transport. Here, we present evidence for a functional link between iron and manganese homeostasis. In the unicellular cyanobacterium, Synechocystis sp. PCC 6803 Fe and Mn deprivation resulted in distinct modifications of the function of the photosynthetic apparatus. For example, iron limitation modifies the rate of QA re-oxidation in photosystem II, a complex that contains more Mn than Fe. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL15867

10 Samples

Download data: TXT

(Submitter supplied) Rre37 activates sugar catabolism in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed rre37-overexpressing strain, named ROX370, by introducing the rre37 genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and ROX370 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed by three times with biologically independent RNA. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL13193

6 Samples

Download data: TXT

(Submitter supplied) RNA samples from cells collected at twenty four time points in 48-hours diurnal cycles were analyzed, and they compared between each other for transcriptional profiles of global genes

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Platform:

GPL21668

24 Samples

Download data: TXT

(Submitter supplied) The unicellular cyanobacterium Synechocystis sp. PCC 6803 is a model system for studying biochemistry, genetics and molecular biology of photobiological processes. Despite its importance in basic and applied research, the genome-wide picture of transcriptional regulation in this bacterium is limited. Characteristic transcriptional responses to changes in the growth environment are expected to provide a scaffold for describing the Synechocystis transcriptional regulatory network as well as efficient means for functional annotation of genes in the genome. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by array

Dataset:

GDS3745

Platform:

GPL7016

27 Samples

Download data: GPR