GEO DataSets

Analysis of sorted spleen T cells of BDC2.5 RAGo/o Foxp3 NOD mice after anti-CD3 treatment. Anti-CD3 treatment prevented diabetes development in BDC2.5.RAGo/o mice. Results provide insight into the mechanism of action of anti-CD3.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE22527

6 Samples

Download data: CEL

(Submitter supplied) Treatment with anti-CD3 is a promising therapeutic approach for autoimmune diabetes, but its mechanism of action remains unclear. Foxp3+ regulatory T (Treg) cells may be involved, but the evidence has been conflicting, and there is great uncertainty as to possible mechanistic connections. We investigated this issue in mice derived from the NOD model, which were engineered mice in which Treg populations were perturbed, or could be manipulated by acute ablation or transfer. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4018

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Foxp3+ regulatory T cells (Treg cells) maintain immunological tolerance and their deficiency results in fatal multi-organ autoimmunity. Although heightened T cell receptor (TCR) signaling is critical for the differentiation of Treg cells, the role of TCR signaling in Treg cell function remains largely unknown. Here we demonstrate inducible ablation of the TCR results in Treg cell dysfunction which cannot be attributed to impaired Foxp3 expression, decreased expression of Treg cell signature genes or altered ability to sense and consume interleukin 2. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

10 Samples

Download data: CEL

(Submitter supplied) Several clinical trials have shown anti-CD3 treatment to be a promising therapy for autoimmune diabetes, but its mechanism of action remains unclear. Foxp3+ regulatory T (Treg) cells are likely to be involved, and we have shown a strong effect of anti-CD3 on homeostatic control of CD4+ FoxP3+ regulatory T (Treg) cells. To analyze the early consequences of anti-CD3 treatment, we sorted and profiled Treg and conventional CD4+ T (Tconv) cells in the first hours and days after anti-CD3 treatment of NOD mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

42 Samples

Download data: CEL

(Submitter supplied) To gain a molecular view of E-proteins with respect to the development of Foxp3+ T cells, we perform microarray studies that would identify transcription factors that are up-regulated as E-proteins levels fall and and Foxp3 expression rises. We hypothesize that such transcription factors activate the synthesis of key proteins necessary for the development of Foxp3+ cells in the thymus (or in the periphery). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

8 Samples

Download data: TXT

(Submitter supplied) Analysis of Foxp3 ablated peripheral regulatory T cells. Regulatory T cells require the expression of the transcription factor Foxp3 for thymic development. It is not known whether continuous expression of Foxp3 is required for the maintained function of mature regulatory T cells in the periphery. Results indicate changes to the regulatory T cell developmental program in the absence of Foxp3. Keywords: genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2525

Platform:

GPL1261

4 Samples

Download data: CEL

Analysis of mature regulatory T cells (Treg) ablated for the transcription factor Foxp3. Foxp3 is required for the development of Treg cells. Results provide insight into the role of Foxp3 in maintaining the transcriptional and functional program established during Treg cell development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL1261

Series:

GSE6681

4 Samples

Download data: CEL

(Submitter supplied) Comparison of gene expression between T regulatory and T effector cells isolated from the pancreatic lesion of 3-4 wk old BDC2.5 tg NOD mice Keywords: cell type comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS684

Platform:

GPL81

30 Samples

Download data: CEL

Comparison of T regulatory (CD4+CD25+CD69-) and T effector (CD4+CD25lo/-CD69+/-) cells isolated from the pancreatic lesion of 3-4 week old BDC2.5 transgenic NOD mice before type 1 diabetes onset.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL81

Series:

GSE1419

8 Samples

Download data: CEL

(Submitter supplied) The CD4+Foxp3+ regulatory T cells play an essential role in maintaining tolerance via their suppressive function on conventional T cells. The intracellular signaling pathways that regulate Foxp3 expression are largely unknown. In this study we describe a novel inhibitory role for AKT in regulating de novo induction of Foxp3 both in vivo and in vitro. A constitutively active allele of AKT significantly diminished TGF-â induced Foxp3 induction via a rapamycin-sensitive pathway, establishing a role for the AKT-mTOR axis in Treg cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Gene expression profiles were compared between regulatory T cells (Treg) and Effector CD4+ T cells in healthy B6 mice and sick mice with scurfy mutation. We used microarrays to elucidate the molecular mechanisms underlying the suppression function of the Treg cells. Keywords: Cell type comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL