GEO DataSets

Analysis of peripheral blood from Salmonella enterica serovar Typhimurium (ST)-inoculated animals differing in Salmonella shedding phenotype at 0 and 2 days post-inoculation (dpi) . Fecal shedding of asymptomatic, Salmonella-carriers can lead to foodborne illness.

Organism:

Sus scrofa

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 10 individual, 2 time sets

Platform:

GPL3533

Series:

GSE27000

20 Samples

Download data: CEL

(Submitter supplied) Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of this gram-negative bacteria in such pigs is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonella shedding following experimental inoculation, we have initiated the first analysis of the whole blood transcriptional response induced by Salmonella. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Dataset:

GDS4246

Platform:

GPL3533

20 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

18 Samples

Download data

(Submitter supplied) Peripheral blood transcriptome is an important intermediate data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four-week-old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood miRNA expression profile before treatment (d0) and at 2 days post inoculation (dpi) using deep sequencing technology.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

6 Samples

Download data: TXT

(Submitter supplied) Peripheral blood transcriptome is an important intermedia data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four weeks old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood gene expression profile before treatment (d0) and at 2 and 7 day post inoculation (dpi) using deep sequencing technology.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL16045 GPL16044

19 Samples

Download data: GPR, TXT

(Submitter supplied) The host response to S.typhimurium infections in ileum was studied after infection of piglets. mRNA and miRNA expression studies were performed to identify interactions of miRNAs and regulated mRNAs in context of mammalian intestinal Salmonella infection using a piglet model.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16045

9 Samples

Download data: GPR

(Submitter supplied) The host response to S.typhimurium infections in ileum was studied after infection of piglets. mRNA and miRNA expression studies were performed to identify interactions of miRNAs and regulated mRNAs in context of mammalian intestinal Salmonella infection using a piglet model.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by array

Platform:

GPL16044

10 Samples

Download data: TXT

(Submitter supplied) To understand the host transcriptional response to S. enterica serovar Choleraesuis (S. Choleraesuis), the first generation Affymetrix porcine GeneChip® was used to identify differentially expressed genes in the mesenteric lymph nodes responding to infection at acute (8 hours (h), 24h, 48h post-inoculation (pi)) and chronic stages (21 days (d) pi) The objectives of this study were to identify and examine the stereotypical gene expression response within the host mesenteric lymph nodes to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by array

Platforms:

GPL15403 GPL14875

17 Samples

Download data: TSV, TXT

(Submitter supplied) This study reports on infection-inducible miRNAs in zebrafish. Using a custom-designed microarray platform for miRNA expression we found that miRNAs of the miR-21, miR-29, and miR-146 families were commonly induced by infection of zebrafish embryos with Salmonella typhimurium and by infection of adult fish with Mycobacterium marinum.

Organism:

Danio rerio

Type:

Non-coding RNA profiling by array

Platform:

GPL15403

9 Samples

Download data: TXT

(Submitter supplied) We used zebrafish embryos as an in vivo system to investigate the role of the microRNA-146 family (consisting of 2 members miR-146a and miR-146b) in the innate immune response to S. typhimurium infection. To determine the role of miR-146 microRNAs in the response to S. typhimurium infection we used Illumina RNA sequencing to compare the mRNA expression profiles of control embryos versus embryos with knockdown of miR-146a and miR-146b. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14875

8 Samples

Download data: TSV

(Submitter supplied) We used microarrays to detail the global programme of gene expression of the mouse colon responses to Salmonella (SL1344 and SB1117) infection at the early phase (8 hours) and the late phase (4 days). We specifically examined the the differentillay gene expression profiles in mouse colon when it responded to pathogenic Salmonella stain SL1344 (with AvrA expression) or SB1117 (without AvrA expression).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

18 Samples

Download data: CEL, CHP

(Submitter supplied) A first generation Affymetrix GeneChip® Porcine genome array was used to profile the gene expression in porcine mesenteric lymph nodes over a time course of infection with S. Typhimurium, including the acute (8 hours post inoculation (hpi), 24 hpi, 48 hpi) and chronic (21 days post-inoculation (dpi)) stages of infection. Our objectives were to 1) identify and examine the stereotypical gene expression response within host MLN to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) HD11 cells were stimulated with 1 ug/ml endotoxin from ST-798 for 1, 2, 4 and 8 hours Cells were harvested at 1, 2, 4 and 8 hrs post stimulation and RNA was isolated from these cells and microarray was conducted for these RNA samples using affymetrix genechips

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

20 Samples

Download data: CEL, TXT

(Submitter supplied) White leghorn layers were infected with Salmonella Enteritidis. The cecum were collected at 7 days post infection for total RNA isolation. The significantly expressed microRNAs between infected and non-infected chickens were identified through Solexa sequencing technology.

Organism:

Gallus gallus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16133

6 Samples

Download data: TXT

(Submitter supplied) To cause a systemic infection, Salmonella must respond to many environmental cues during mouse infection and express specific subsets of genes in a temporal and spatial manner, but the regulatory pathways are poorly established. To unravel how micro-environmental signals are processed and integrated into coordinated action, we constructed in-frame non-polar deletions of 83 regulators inferred to play a role in Salmonella enteriditis Typhimurium (STM) virulence and tested them in three virulence assays (intraperitoneal [i.p.], and intragastric [i.g.] infection in BALB/c mice, and persistence in 129X1/SvJ mice). more...

Organism:

Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S

Type:

Expression profiling by array

Platform:

GPL4804

55 Samples

Download data: TXT

(Submitter supplied) To adapt the lives of organisms to the day-night cycle, evolution has built a complex machinery, whose molecular components are able to anticipate and drive changes in organism behavior and metabolism. A mutual bidirectional interaction exists between circadian abnormalities and development of diseases. Microarrays were used to determine whether circadian regulation occurs at the transcriptional level in response to Salmonella Typhimurium infection.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4622

Platform:

GPL6246

25 Samples

Download data: CEL

Analysis of cecum from master circadian regulator Clock mutants infected with S. Typhimurium at two circadian times (10am and 10pm). Clock mutants infected at night exhibited higher inflammation. Results provide insight into the role of the circadian clock in the host immune response to infection.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 infection, 2 time sets

Platform:

GPL6246

Series:

GSE46356

25 Samples

Download data: CEL

(Submitter supplied) Piglets were raised in isolation facilities from 10 days to 7 weeks of age at the National Animal Disease Center. Bacteriological culture of rectal swabs was performed twice to confirm that all pigs were free of Salmonella spp. Three control pigs were necropsied on experimental day -3. On day 0, pigs in the infected group were intranasally challenged with 1 x 109 CFU S. Choleraesuis X3246. Lung samples were aseptically collected from three 24 h and three 48 h post infection pigs and immediately frozen in liquid nitrogen for future mRNA isolation. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL1881

9 Samples

Download data