GEO DataSets

Analysis of hippocampi from cellular prion protein (PrPC)-deficient, FVB newborns (4.5-day-old) and adults (3-mo-old). PrPc protein is widely expressed throughout the CNS; mice lacking PrP are resistant to prion infection. Results provide insight into the influence of PrPC on the developing CNS.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 development stage, 2 genotype/variation sets

Platform:

GPL8321

Series:

GSE21718

14 Samples

Download data: CEL

(Submitter supplied) A key event in the pathogenic process of prion diseases is the conversion of the cellular prion protein (PrPC) to an abnormal and protease-resistant isoform (PrPSc). Mice lacking PrP are resistant to prion infection, and down-regulation of PrPC during prion infection prevents neuronal loss and the progression to clinical disease. These results are suggestive of the potential beneficial effect of silencing PrPC during prion diseases. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4352

Platform:

GPL8321

14 Samples

Download data: CEL

(Submitter supplied) We demonstrate that Prnp dosage is critical for the maintenance of neuronal homeostasis since both its absence and, more relevantly, its overexpression induce higher sensitivity to kainate (KA) damage. These data correlate with electrophysiological results in freely behaving mutant mice showing an imbalance in activity-dependent synaptic processes, as determined from input/output curves, paired-pulse facilitation, and LTP studies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

12 Samples

Download data: TXT

(Submitter supplied) Purpose: Gain insight into the gene expression patterns altered in the PrnpZH3/ZH3 mice, that correleate with their neurobehavioural impaiments. Results: Around 700 genes showed alterations in their expression profile (323 upregulated and 390 downregulated in PrnpZH3/ZH3 compared to Prnp+/+).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

16 Samples

Download data: TXT, XLSX

(Submitter supplied) Prion infection results in progressive neurodegeneration of the central nervous system invariably resulting in death. The pathological effects of prion diseases in the brain are morphologically well defined, such as gliosis, vacuolation, and the accumulation of disease-specific protease-resistant prion protein (PrPSc). However, the underlying molecular events that lead to the death of neurons are poorly characterised. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6412 GPL4134

39 Samples

Download data: TXT

(Submitter supplied) Prions consist of aggregates of abnormal conformers of cellular prion protein (PrPC). They propagate by recruiting host-encoded PrPC although the critical interacting proteins and the reasons for the differences in susceptibility of distinct cell lines and populations are unknown. We derived a lineage of cell lines with markedly differing susceptibilities, unexplained by PrPC expression differences, to identify such factors. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

41 Samples

Download data: CEL

(Submitter supplied) Prion diseases typically have long pre-clinical incubation periods during which time the infectious prion particle and infectivity steadily propagate in the brain. Abnormal neuritic sprouting and synaptic deficits are apparent during pre-clinical disease, however, gross neuronal loss is not detected until the onset of the clinical phase. The molecular events that accompany early neuronal damage and ultimately conclude with neuronal death remain obscure. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

38 Samples

Download data: TXT

(Submitter supplied) Wild-type zebrafish larvae and our prp1ua5003/ua5003;prp2ua5001/ua5001 mutant zebrafish larvae at 3 days post fertilisation were pooled into three biological replicates each of 50 larvae. Each replicate underwent paired end 100-125 and Illumina HiSeq2500 sequencing to a depth of 40 million reads, carried out by Otogenetics (Atlanta, GA). Samples were subsequently processed using the TopHat/Cufflinks bioinformatics pipeline. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18413

6 Samples

Download data: CSV

(Submitter supplied) To study the effect of Prnp genetic ablation on different aspects of RNA metabolism, we performed RNA sequencing of the hippocampus of wild-type C57BL/6J, congenic B6.129-PrnpZH1/ZH1 and coisogenic C57BL/6J-PrnpZH3/ZH3 mice. We analyzed differential gene expression, exon usage and RNA editing.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) Prion disease is caused by misfolding of the prion protein (PrP) into pathogenic self-propagating conformations, leading to rapid onset dementia and death. However, elimination of endogenous PrP can halt prion disease progression. Here, we describe CHARM, a compact, enzyme-free epigenetic editor capable of silencing transcription through programmable targeted DNA methylation. Using a histone H3 tail-Dnmt3l fusion, CHARM recruits and activates the endogenous DNA methyltransferases, thereby reducing transgene size and bystander effects. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: TXT

(Submitter supplied) A comparison of prion infected and non-infected samples from neuroblastoma cells (N2a), and a comparison of prion infected and non-infected samples from hypothalmus cells (GT1). 11 dual-color DNA-chip hybridizations of cDNAs were made. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1413

11 Samples

Download data

(Submitter supplied) The underlying pathogenic mechanisms of prion infection are not well characterized. To study the effect of prion infection on gene expression in neuronal cell cultures, a neuroblastoma (N2a) cell clone was infected with either the mouse adapted prion strain 22L or exposed to uninfected brain homogenate as a negative control. Large scale expression analysis was performed using a cDNA microarray chip comprising about 21,000 spotted ESTs. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3697

8 Samples

Download data: GPR

(Submitter supplied) Affymetrix genechip profiling analsysis (MOE430A and MOE430B) of murine neuroblastoma cells infected with either RML prion strain or mock brain homogenate Keywords: Affymetrix gene expression profiling

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL340 GPL339

12 Samples

Download data: CEL

(Submitter supplied) The transcriptome of sciatic nerves was investigated by RNA sequencing. We compared the transcriptome of ZH3 sciatic nerves to WT sciatic nerves in two age groups. Differentially expressed genes were considered as indicators of peripheral nerve damage in ZH3 mice. Then, we compared the sciatic nerve transcriptome of ZH3 mice chronically treated with a dimeric PrP-fusion protein (FT2Fc) to the two control groups treated with either buffer or mouse IgG. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

26 Samples

Download data: TXT