GEO DataSets

Analysis of embryonic day 10.5 (E10.5) embryos collected from streptozotocin-induced diabetic FVB females. Maternal diabetes is a known risk factor for birth defects. Results provide insight into molecular mechanisms underlying developmental abnormalities of embryos of diabetic pregnancies.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL1261

Series:

GSE41095

6 Samples

Download data: CEL

(Submitter supplied) Exposure to maternal diabetes during pregnancy alters transcriptional profiles in the developing embryo. The enrichment, within the set of de-regulated genes, of those encoding transcriptional regulatory molecules provides support for the hypothesis that maternal diabetes affects specific developmental programs. We compared E10.5 cntrol embryos to E10.5 embryos from diabetic pregnancies in the FVB mouse strain.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4590

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Diabetic embryopathy can affect any developing organ system, although cardiovascular malformations, neural tube defects and caudal dysgenesis syndrome are the most prominent congenital malformations. We hypothesize that the metabolic imbalance occurring in diabetic pregnancy de-regulates tissue specific gene expression programs in the developing embryo. In order to identify genes whose expression is affected by maternal diabetes, we analyzed gene expression profiles of diabetes-exposed mouse embryos by using Affymetrix microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4028

Platform:

GPL339

7 Samples

Download data: CEL, CHP

Analysis of diabetes-exposed, E10.5 whole embryos. Diabetes was induced in FVB females (aged 7-9 wks) by streptozotocin. Maternal diabetes is a well-known risk factor for birth defects, such as neural tube defects. Results provide insight into the causative molecular mechanisms in developing embryo.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 disease state, 2 stress sets

Platform:

GPL339

Series:

GSE9675

7 Samples

Download data: CEL, CHP

(Submitter supplied) The reduced folate carrier (RFC1) is an integral membrane protein and facilitative anion exchanger that mediates delivery of 5-methyltetrahydrofolate into mammalian cells. Adequate maternal-fetal transport of folate is necessary for normal embryogenesis. Targeted inactivation of the murine RFC1 gene results in post-implantation embryo lethality, but daily folic acid supplementation of pregnant dams prolongs survival of homozygous embryos until mid-gestation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Congenital heart defects (CHD) are one of the most common defects in offspring of diabetic mothers. There is a clear association between maternal diabetes and CHD; however the underlying molecular mechanism remains unknown. We hypothesized that maternal diabetes affects with the expression of early developmental genes that regulate the essential developmental processes of the heart, thereby resulting in the pathogenesis of CHD. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4594

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

Analysis of pooled E13.5 or E15.5 embryonic heart tissues from diabetic dams. Congenital heart defects (CHD) are one of the most common defects in offspring of diabetic mothers. Results provide insight into molecular changes in embryonic heart contributing to CHD offspring of diabetic pregnancies.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 age, 2 disease state sets

Platform:

GPL1261

Series:

GSE32078

12 Samples

Download data: CEL, CHP

(Submitter supplied) Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the underlying mechanism is still unclear. To address this question, we analysed gene expression profiles of SCNT-derived 2-cell mouse embryos treated with trichostatin A (TSA), a potent HDAC inhibitor that is best used for mouse cloning. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

39 Samples

Download data: TXT

(Submitter supplied) Gene regulation at the maternal-embryonic transition in the pre-implantation mouse embryo is not well understood. We knock down Ccna2 to establish proof-of-concept that antisense morpholino oligonucleotides can be used to target specific genes. We applied this strategy to study Oct4 and discovered that Oct4 is required prior to blastocyst development. Specifically, gene expression is altered as early as the 2-cell stage in Oct4-knockdown embryos. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) The objective of the present study was to use our own fabricated bovine embryo cDNA microarray to profile genome-wide expression patterns of genes during the implantation period. Duplicate readings were obtained by hybridization of a Cy3/Cy5 probe mixture for each sample. Data normalization was performed the local background intensity of each array spot was smoothed by the local weight regression (lowess) smoother and subtracted from spot intensity data. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS1003

Platform:

GPL1221

11 Samples

Download data: TIFF

Expression profiling of embryos at various days of gestation up to 21 days and fetuses and fetal membranes at 27 and 28 days of gestation. Results provide insight into the regulation of embryonic, fetal, and fetal membrane differentiation.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 7 development stage, 3 tissue sets

Platform:

GPL1221

Series:

GSE1414

11 Samples

Download data: TIFF

(Submitter supplied) 22q11-deletion syndrome (22q11DS) is a developmental anomaly caused by a microdeletion on human chromosome 22q11. Although mouse models indicated Tbx1 as the gene responsible of the syndrome, the phenotypic spectrum of del22q11 patients is complex suggesting that gene-gene and gene-environment interactions, probably during embryonic development, are crucial in delineating the pathogenesis of 22q11DS. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3634

14 Samples

Download data

(Submitter supplied) The whole embryo culture (WEC) model serves as a potential replacement for classical in vivo developmental toxicity testing. In this alternative test, cultured rat embryos are exposed during neurulation and early organogenesis and evaluated for morphological adverse outcomes. Toxicogenomic-based approaches may improve the predictive ability of WEC by providing molecular-based markers associated with chemical exposure which can be compared across multiple parameters (e.g. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL14783

204 Samples

Download data: CEL

(Submitter supplied) Maternal diabetes during early pregnancy can cause birth defects, notably of the developing heart and nervous system. To recognize the gene expression differences that may be the basis for such birth defects, we performed RNA-Seq on mouse embryos of the Non-Obese Diabetic (NOD) strain. Mice of that strain spontaneously develop type I diabetes.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

136 Samples

Download data: TXT

(Submitter supplied) Using microarrays, genome-wide RNA expression was profiled and compared for in vitro fertilization (IVF) - derived blastocysts and embryos undergoing degenerative development up to the same time point. Surprisingly similar transcriptomic profiles were found in degenerative embryos and blastocysts. Nonetheless, we identified 67 transcripts that significantly differed between these two groups of embryos at a 15% false discovery rate, including 33 transcripts showing at least a two-fold difference. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

6 Samples

Download data: CEL

(Submitter supplied) The putative transcription factor, activity-dependent neuroprotective protein (ADNP) is a zinc finger and homeodomain - like profile containing protein. ADNP knockout mice die at day 9 of gestation. To reveal ADNP-associated pathways, a 22,690- Affymetrix probe array was used on ADNP knockout and control embryos. References: Complete sequence of a novel protein containing a femtomolar-activity-dependent neuroprotective peptide. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2540

Platform:

GPL339

7 Samples

Download data

Analysis of E9 mutant embryos lacking the gene for the activity-dependent neuroprotective protein (ADNP). ADNP deficiency results in neural tube closure defects and death at E8.5 to E9.5. Results provide insight into the role of ADNP in embryogenesis at the molecular level.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL339

Series:

GSE4068

7 Samples

Download data

(Submitter supplied) AIMS/HYPOTHESIS: Pregnancies complicated by diabetes have a higher risk of adverse outcomes for mothers and children, including predisposition to disease later in life, such as metabolic syndrome and hypertension. We hypothesized that adverse outcomes from diabetic pregnancies may be linked to compromised placental function. Our goal in this study was to identify cellular and molecular abnormalities in diabetic placenta. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3989

Platform:

GPL1261

10 Samples

Download data: CEL, CHP, TXT

Analysis of placentae (including decidua) of streptozotocin-induced diabetic FVB dams at gestational day E10.5. Adverse outcomes from diabetic pregnancies may be linked to compromised placental function. Results provide insight into molecular basis of placental abnormalities in diabetic pregnancies.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL1261

Series:

GSE28277

10 Samples

Download data: CEL, CHP

(Submitter supplied) Cloned embryos produced by somatic cell nuclear transfer (SCNT) display a plethora of phenotypic characteristics that make them different from fertilized embryos, indicating defects in the process of nuclear reprogramming by the recipient ooplasm. To elucidate the extent and timing of nuclear reprogramming, we used microarrays to analyze the transcriptome of mouse SCNT embryos during the first two cell cycles. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

40 Samples

Download data: CEL