GEO DataSet Links for PMC (Select 7755741) - GEO DataSets

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Items: 2

Select item 2001535121.

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and METTL14 KO bone marrow-derived macrophages(BMDM) Transcriptomes

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived BMDM transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: BMDM mRNA profiles of 6-week-old wild-type (WT) and METTL14 knockout (M14−/−) mice with or without LPS treatment were generated by deep sequencing, in triplicate, using Illumina Hi-Seq 4000. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21103
12 Samples

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Accession:: GSE153512

ID:: 200153512

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001535112.

m6A modification maps of mRNA in bone marrow-derived macrophages (BMDMs) from mouse

(Submitter supplied) We report the application of sequencing technology for high-throughput profiling of mRNA m6A methylation in mice BMDMs with or without PBS treatment. By obtaining over thirty billion bases of sequence from m6A antibody immunoprecipitated mRNA, we generated m6A methylation maps of mouse BMDMs in the presence or absence of LPS. We find that SOCS1 mRNA methylation is robustly enhanced by LPS treatment in WT BMDMs, leading to the increase of SOCS1 expression. more...