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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 09, 2020 |
| Title |
m6A modification maps of mRNA in bone marrow-derived macrophages (BMDMs) from mouse |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
We report the application of sequencing technology for high-throughput profiling of mRNA m6A methylation in mice BMDMs with or without PBS treatment. By obtaining over thirty billion bases of sequence from m6A antibody immunoprecipitated mRNA, we generated m6A methylation maps of mouse BMDMs in the presence or absence of LPS. We find that SOCS1 mRNA methylation is robustly enhanced by LPS treatment in WT BMDMs, leading to the increase of SOCS1 expression. However, LPS challenge fails to increase SOCS1 expression in the M14-/- BMDMs, indicating m6A modification plays a cirital role in the SOCS1 expression. This study provides a framework for the application of comprehensive mRNA m6A profiling towards characterization of BMDMs.
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| Overall design |
Examination of mRNA m6A modifications in mice BMDMs with or without LPS treatment.
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| Contributor(s) |
Li Y, Du J, Liao W |
| Citation(s) |
33220174 |
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| Submission date |
Jun 29, 2020 |
| Last update date |
Dec 09, 2020 |
| Contact name |
Jie Du |
| E-mail(s) |
[email protected]
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| Organization name |
University of Chicago
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| Street address |
900 E. 57th Street
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| City |
Chicago |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA642873 |
| SRA |
SRP269253 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE153511_Macrophage_mettl14_sig_diff_peak_annotated.xlsx |
337.5 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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