GEO DataSets

(Submitter supplied) This dataset profiles the transcriptional response of in vitro polarized TH2 cells treated with calcitriol (active vitamin D) and vehicle controls. RNA-seq was used to identify differentially expressed genes influenced by vitamin D signaling. This study provides insight into the molecular mechanisms through which vitamin D modulates immune responses in TH2 cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

22 Samples

Download data: CSV

(Submitter supplied) We have completed the Human lncRNA microarray analysis of the 4 samples that you submitted. Total RNA from each sample was quantified using the Nanodrop 1000 and the RNA integrity was assessed by Agilent 2100 Bioanalyzer. About 5 μg total RNA of each sample was used for labeling and array hybridization as the following steps: 1) Reverse transcription with invitrogen Superscript ds-cDNA synthesis kit; 2) ds-cDNA labeling with Nimblegen one-color DNA labeling kit; 3) Array hybridization using the NimbleGen Hybridization System and followed by washing with the Nimblegen wash buffer kit. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL35135

4 Samples

Download data: CALLS, PAIR, XLS

(Submitter supplied) The combination of decreasing food intake and increasing energy expenditure represents a powerful strategy for counteracting cardiometabolic diseases, such as obesity and type 2 diabetes1. Yet current approaches require conjugating multiple receptor agonists to achieve both effects2–4 and, thus far, no safe energy-expending option has reached the clinic. Here we show that activation of Neurokinin 2 Receptor (NK2R) is sufficient to suppress appetite centrally and increase energy expenditure peripherally. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: CSV, MTX, TSV

(Submitter supplied) Vitamin A, predominantly present as all-trans retinol, is an essential nutrient with wide-ranging physiological roles in embryonic development, vision, immunity, reproduction, cell differentiation and proliferation, apoptosis and maintenance of epithelial integrity. In this study the effects of an increased dietary Vitamin A on the regeneration of urinary bladder urothelial cells after severe cyclophosphamide (CP)-induced injury are investigated, focusing on proliferation, apoptosis and differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

18 Samples

Download data: TXT

(Submitter supplied) Low frequency Jak2VF mutations promote atherosclerosis via IL-1 mediated cross-talk. Therapeutic approaches that increase MERTK or TREM2 could promote plaque stabilization in inflammasome-driven atherosclerosis.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: MTX, TSV

(Submitter supplied) Human diet emerges as a pivotal determinant of gut microbiota composition and function. Identification of the bacterial taxa targeted by diet derived factors with causal beneficial rather than detrimental effects on therapy and their mechanism of action is challenging but necessary for future clinical progress. The germ free mice colonized with human gut bacteria and four-plants derived nanoparticles uptaking bacteria were sorted with flow cytometry and identified with 16s rRNA next-generation sequencing.

Organism:

feces metagenome

Type:

Other

Platform:

GPL24116

77 Samples

Download data: XLSX

(Submitter supplied) Ethanolamine phosphate phospholyase (ETNPPL) is an enzyme that irreversibly degrades phospho-ethanolamine (p-ETN), an intermediate in the Kennedy pathway of phosphatidylethanolamine (PE) biosynthesis. PE is the second most abundant phospholipid in mammalian membranes. Disturbance of hepatic phospholipid homeostasis has been linked to the development of metabolic dysfunction-associated steatotic liver disease (MASLD). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

10 Samples

Download data: TXT

(Submitter supplied) Cigarette smoke has been demonstrated to stimulate the growth of existing bladder tumors by enhancing the survival and proliferation of cancer cells through the action of carcinogens present in smoke. Concurrently, Fatty Acid Synthase (FASN), a key enzyme in fatty acid synthesis crucial for lipid metabolism, exhibits dysregulation in various cancer types, often correlating with aggressive phenotypes. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

3 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) Rheumatoid arthritis (RA) is a common chronic autoimmune and inflammatory disease that can cause joint pain and disability. We have recently reported that oral magnesium supplementation significantly reduces disease severity and joint damage in two models of RA. In the synovial tissues. There was an enrichment for differentially expressed genes (DEGs) belonging to pathways implicated in transcription regulation, nuclear receptor signaling, and pathways implicated in RA pathogenesis such as RHO GTPases, RUNX1 pathway, oxidative stress induced senescence, senescence-associated secretory phenotype (SASP) and cell migration in synovial tissues . more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

26 Samples

Download data: XLS

(Submitter supplied) The objective of this paper was to determine whether shifts in the methylome in rainbow trout (Oncorhynchus mykiss) are correlated with transcriptomic changes during early development in response to maternal dietary choline intake. Three experimental diets were formulated to have different levels of choline: (a) 2065 ppm choline (Low Choline, 0% supplementation), (b) 5657 ppm choline (Medium Choline, 0.6% supplementation), and (c) 9248 ppm choline (High Choline, 1.2% choline supplementation). more...

Organism:

Oncorhynchus mykiss

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL23004

17 Samples

Download data: TXT, XLSX

(Submitter supplied) To determine the effect of SphK2 KO on liver metabolic-associated gene expression in an early MASH mouse model To determine the effect of WDSW on liver metabolic-associated gene expression in an early MASH mouse model

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL35014

12 Samples

Download data: RCC

(Submitter supplied) To determine the effect of SphK2 KO on liver inflammation in an early MASH mouse model To determine the effect of WDSW on liver inflammation in an early MASH mouse model

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL35013

12 Samples

Download data: RCC, XLSX

(Submitter supplied) RNA seq was applied to the lung and liver tissues from mice with different treatment regimen to evaluate the potential effects on global transcription.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

29 Samples

Download data: CSV

(Submitter supplied) Genome-scale metabolic network models (MNMs) can be used with a mathematical modeling tool called flux balance analysis to gain insights into metabolism at a systems level. Such models represent metabolism of an organism as a whole and do not automatically reveal how the flux overall is wired, i.e., which reactions carry flux, and which do not, and measuring flux at a large scale is challenging. Enzyme levels, as proxied by mRNA levels, can be used to predict reaction fluxes. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL25145 GPL27998

10 Samples

Download data: CSV

(Submitter supplied) The black soldier fly (Hermetia illucens) is important in antimicrobial peptides (AMP) research due to its exposure to diverse microbial environments. However, the impact of different fungal exposures on AMP abundance in H. illucens has not been thoroughly explored. Our study focused on basal conditions and interactions with three fungi: the non-pathogenic Candida tropicalis (isolated from larval gut), Saccharomyces cerevisiae, and the pathogenic Beauveria bassiana. more...

Organism:

Hermetia illucens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34702

16 Samples

Download data: CSV

(Submitter supplied) Inositol pyrophosphates (PP-IPs) regulate diverse physiological processes. Mammalian genome encodes three enzymes (IP6K1-3) synthesizing PP-IP diphosphoinositol pentakisphosphate (IP7). The objective of this experiment is to elucidate the physiological roles of IP6K2-dependent PP-IPs metabolism in gene expression of central nervous system and gastrointestinal tract. Mouse GE 4×44K v2 microarray was employed to comprehensively analyze mRNA expression in spinal cord and duodenum. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

4 Samples

Download data: TXT

(Submitter supplied) Non-alcoholic fatty liver disease (NAFLD) is a growing health problem worldwide, ranging from non-alcoholic fatty liver (NAFL) to the more severe metabolic non-alcoholic steatohepatitis (NASH). Although many studies have elucidated the pathogenesis of NAFLD, the epigenetic regulatory mechanism from NAFL to NASH remains incompletely understood. The histone H3 lysine 4 methyltransferase, MLL4 (also called KMT2D), is a critical epigenetic transcriptional coactivator that mediates overnutrition-induced steatosis in mice, but its potential role in the progression of NASH remains largely unknown. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

7 Samples

Download data: XLSX

(Submitter supplied) Objective: The early consumption of calorie-rich diet disrupts circadian rhythms and has adverse effects on memory, yet the effects of timerestricted feeding (TRF) and the underlying molecular mechanisms are unknown. Here, we set out to identify the behavioral and molecular circadian rhythms disruptions generated by juvenile obesogenic diet consumption and their restoration by TRF in male mice. Methods: Metabolic rhythms were measured by indirect calorimetry and memory performances by behavioral tasks. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30172

71 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to identify novel key genes and to investigate pathways involved in effect of iron-overload on atherosclerosis

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: TXT

(Submitter supplied) Mammals evolved to withstand frequent fasting periods due to hepatic production of glucose and ketone bodies. Because the fasting response is transcriptionally-regulated, we asked whether enhancer dynamics impose a transcriptional program during recurrent fasting and whether this produces effects distinct from a single fasting bout. We found that mice undergoing alternate-day fasting (ADF) respond to a following fasting bout profoundly differently from mice first experiencing fasting. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL21626 GPL19057

34 Samples

Download data: BEDGRAPH