GEO DataSets

(Submitter supplied) Polyamines, crucial molecules involved in cell proliferation and growth, play a pivotal role in cancer development and progression. Within the tumor microenvironment, macrophages, key components of the immune system, exhibit a complex relationship with polyamines. Evidence suggests that polyamines can modulate macrophage polarization, influencing their functional phenotypes. Here, we detected the gene DNA methylation changes in spermine-stimulated human macrophages isolated from PBMCs and TAMs.

Organism:

Leptospira interrogans; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis; Mycobacterium tuberculosis variant microti; Mycobacterium canetti; Orthohantavirus seoulense; Yersinia enterocolitica; Toxoplasma gondii; Salmonella enterica subsp. enterica serovar Typhimurium; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Campylobacter jejuni; Francisella tularensis subsp. novicida; Yersinia pestis; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. mediasiatica; Paslahepevirus balayani; Yersinia pseudotuberculosis; Rickettsia prowazekii; Bartonella quintana; Mycobacterium avium; Homo sapiens; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica

Type:

Methylation profiling by array

Platform:

GPL21445

4 Samples

Download data: IDAT, TXT

(Submitter supplied) To assess which F. tularensis LVS transcripts are impacted by loss of bS21-2, we generated cells lacking the gene encoding bS21-2 (LVS ∆rpsU2). We then grew wild-type cells containing an empty vector (LVS pF), cells lacking bS21-2 with empty vector (LVS ∆rpsU2 pF), and cells lacking the native bS21-2 but ectopically expressing bS21-2 from a plasmid (LVS ∆rpsU2 pF-rpsU2-V). We isolated RNA from mid-log phase cells and analyzed by RNA-Seq.

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32555

7 Samples

Download data: TXT

(Submitter supplied) Francisella tularensis is a highly infectious zoonotic pathogen with as few as 10 organisms causing tularemia, a disease that is fatal if untreated. Although F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B) share over 99.5% average nucleotide identity, notable differences exist in genomic organization and pathogenicity. The type A clade has been further divided into subtypes A.I and A.II, with A.I strains being recognized as some of the most virulent bacterial pathogens known. more...

Organism:

Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32256 GPL32255

96 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Francisella tularensis is a Gram-negative bacterium that causes a fatal human disease known as tularemia. The Centers for Disease Control have classified F. tularensis as Category A Tier-1 Select Agent. The virulence mechanisms of Francisella are not entirely understood. Francisella possesses very few transcription regulators, and most of these regulate the expression of genes involved in intracellular survival and virulence. more...

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23586

12 Samples

Download data: XLS

(Submitter supplied) We used transposon insertion sequencing (Tn-Seq) to identify the genes that are required for in vitro growth and intramacrophage growth of the live vaccine strain of F. tularensis (LVS).

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Other

Platform:

GPL27588

5 Samples

Download data: WIG

(Submitter supplied) The goal of this study is to compare transcriptomics between wild type LVS vs AR-13 isogenic resistant mutant

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23586

10 Samples

Download data: XLSX

(Submitter supplied) A microarray was developed to screen rodent samples for pathogens of zoonotic importance In the work described here, a homologue to Yersinia pestis was found in rodent samples after screening with the microarray

Organism:

Leptospira interrogans; Francisella tularensis subsp. novicida; Yersinia pseudotuberculosis; Mycobacterium avium subsp. paratuberculosis; Mycobacterium tuberculosis; Mus musculus; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Paslahepevirus balayani; Bartonella quintana; Staphylococcus aureus; Mycobacterium avium; Rattus norvegicus; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. holarctica; Campylobacter jejuni; Yersinia pestis; Mycobacterium tuberculosis variant microti; Rattus rattus; Mycobacterium canetti; Francisella tularensis subsp. mediasiatica; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Orthohantavirus seoulense; Yersinia enterocolitica; Rickettsia prowazekii; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Toxoplasma gondii; Apodemus sylvaticus; Salmonella enterica subsp. enterica serovar Typhimurium

Type:

Genome variation profiling by array

Platform:

GPL21445

65 Samples

Download data: TXT

(Submitter supplied) Comparison of expression profiles of strains of Francisella to identify virulence factors We used custom microarrays to detail the global gene expression of four strains of Francisella (Schu4, LVS, OR960246, U112) and identified distinct classes of differentially expressed genes during this process.

Organism:

Francisella tularensis subsp. holarctica LVS; Francisella tularensis subsp. holarctica OR96246; Francisella tularensis subsp. tularensis SCHU S4; Francisella; Francisella tularensis subsp. novicida U112

Type:

Expression profiling by array

Platform:

GPL20119

12 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of LVS grown in hepatocytic cell line FL83B was compared to that of LVS cultued in MH broth to discover genes important for intracellular growth.

Organism:

Francisella tularensis; Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by array

Platform:

GPL4250

4 Samples

Download data: GPR

(Submitter supplied) Oligonucleotides were purchased as 3′-amino-modified oligos from Metabion (Martinsried, Germany) and applied at a final concentration of 10 μM in Spotting Buffer 1 (Quantifoil Micro Tools, Jena, Germany). Arrays were spotted using a Microgrid II spotting machine (BioRobotics Inc., Cambridge, UK) according to the instructions of the manufacturer and assembled into ArrayStrips. Each oligonucleotide probe was replicated two-fold on the array. more...

Organism:

Leptospira interrogans; Campylobacter jejuni; Francisella tularensis subsp. novicida; Yersinia enterocolitica; Yersinia pestis; Yersinia pseudotuberculosis; Rickettsia prowazekii; Rickettsia typhi; Bartonella quintana; Staphylococcus aureus; Mycobacterium avium; Mycobacterium tuberculosis variant bovis; Mycobacterium avium subsp. paratuberculosis; Mycobacterium tuberculosis; Mycobacterium tuberculosis variant microti; Toxoplasma gondii; Cowpox virus; Streptobacillus moniliformis; Bartonella henselae; Mycobacterium canetti; Escherichia coli O157:H7; Salmonella enterica subsp. enterica serovar Typhimurium; Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica; Francisella tularensis subsp. mediasiatica; Paslahepevirus balayani; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Orthohantavirus seoulense

2 Series

69 Samples

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