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Series GSE202948 Query DataSets for GSE202948
Status Public on Jul 13, 2022
Title Arginine catabolism and polyamine biosynthesis pathway disparities within Francisella tularensis subpopulations
Organisms Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica
Experiment type Expression profiling by high throughput sequencing
Summary Francisella tularensis is a highly infectious zoonotic pathogen with as few as 10 organisms causing tularemia, a disease that is fatal if untreated. Although F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B) share over 99.5% average nucleotide identity, notable differences exist in genomic organization and pathogenicity. The type A clade has been further divided into subtypes A.I and A.II, with A.I strains being recognized as some of the most virulent bacterial pathogens known.
In this study, we report on major disparities that exist between the F. tularensis subpopulations in arginine catabolism and subsequent polyamine biosynthesis. The genes involved in these pathways include the speDEA and aguAB operons, along with metK. In the hypervirulent F. tularensis A.I clade, such as the A.I prototype strain SCHU S4, these genes were found to be intact and highly transcribed. In contrast, both subtype A.II and type B strains have a truncated speA gene, while the type B clade also has a disrupted aguA and truncated aguB. Ablation of the chromosomal speE gene that encodes a spermidine synthase reduced subtype A.I SCHU S4 growth rate, whereas the growth rate of type B LVS was enhanced. These results demonstrate that spermine synthase SpeE promotes faster replication in the F. tularensis A.I clade, whereas type B strains do not rely on this enzyme for fitness. Our ongoing studies on metabolism should provide a better understanding of the factors that contribute to F. tularensis pathogenicity.
 
Overall design Comparative gene expression profiling analysis of RNA-Seq data for F. tularensis subtype A.I strains SCHU S4 and MA00-2987, subtype A.II strain WY96-3418, and type B LVS during mid-log growth in brain heart infusion (BHI) broth.
 
Contributor(s) Larson MA, Sayood K, Mansouri A, Moore R, Helikar T
Citation(s) 35794913
Submission date May 13, 2022
Last update date Jul 13, 2022
Contact name Robert Bristow Moore
E-mail(s) [email protected]
Organization name University of Nebraska - Lincoln
Department Biochemistry
Lab Helikar Lab
Street address 1901 vine street
City LINCOLN
State/province NE
ZIP/Postal code 68504
Country USA
 
Platforms (2)
GPL32255 Illumina NextSeq 500 (Francisella tularensis subsp. holarctica)
GPL32256 Illumina NextSeq 500 (Francisella tularensis subsp. tularensis)
Samples (96)
GSM6139073 LVS, rep1
GSM6139074 LVS, rep2
GSM6139075 LVS, rep3
Relations
BioProject PRJNA837875

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Supplementary file Size Download File type/resource
GSE202948_RAW.tar 5.4 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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