GEO DataSets

(Submitter supplied) Zn and Mn are essential micronutrients for many bacteria including Streptococcus pneumoniae. While Zn performs vital structural or catalytic roles in certain proteins, in excess, Zn can inhibit Mn uptake by S. pneumoniae and displace, but not functionally replace Mn from key enzymes including superoxide dismutase A (SodA). Here, we show that the Ccn small regulatory RNAs promote S. pneumoniae Mn uptake and resistance to the oxidative stress. more...

Organism:

Streptococcus pneumoniae TIGR4; Streptococcus pneumoniae D39

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33901 GPL22373

18 Samples

Download data: XLSX

(Submitter supplied) Previous studies on RNase R have highlighted significant effects of this ribonuclease in several processes of Streptococcus pneumoniae biology. In this work we show that elimination of RNase R results in overexpression of most of genes encoding the components of type II fatty acid biosynthesis (FASII) cluster. We demonstrate that RNase R is implicated in the turnover of most of transcripts from this pathway, affecting the outcome of the whole FASII cluster, and ultimately leading to changes in the membrane fatty acid composition. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23152

4 Samples

Download data: TXT

(Submitter supplied) Cell-cell communication systems enable populations to regulate behavior via modifications in gene expression. In Gram-positive bacteria, these systems are often composed of secreted, signaling peptides that disperse across the population and interact with cognate transcriptional regulators. While cells generally possess multiple such systems, there is a gap in knowledge regarding molecular mechanisms that coordinate these systems to allow for optimization of phenotypic responses. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

8 Samples

Download data: CSV

(Submitter supplied) This study investigates the regulons of 11 pneumococcal response regulators (RRs) by RNA-sequencing of RR-overexpression mutants.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

36 Samples

Download data: CSV

(Submitter supplied) Streptococcus pneumoniae (Spn), a Gram-positive bacterium, poses a significant threat to human health, causing mild respiratory infections to severe invasive conditions. Despite availability of vaccines, challenges persist due to serotype replacement and antibiotic resistance, emphasizing the need for alternative therapeutic strategies. This study explores the intriguing role of polyamines, ubiquitous, small organic cations, in modulating virulence factors, especially the capsule, a crucial determinant of Spn's pathogenicity. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34053

12 Samples

Download data: CSV

(Submitter supplied) The gene expression of S. pneumoniae cultured in medium with different concentration of phosphate were detected, aiming to analyze the role of reduced extracellular phosphate in virluence regulation of S. pneumoniae.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23152

2 Samples

Download data: CSV, XLS

(Submitter supplied) We obtained the topARA mutant that encodes a TopoI enzyme that shows reduced topoisomerase activity compared to the wild type gene (topAWT) and confers resistance to the antibiotic seconeolitsine. Alterations in the gene expression profile produced after overexpression of the wild type or the RA mutant of topA gene were studied. In addition, differential gene expression produced by the DNA gyrase inhibitor novobiocin (NOV) was also studied in cultures overexpressing each of the versions of TopA.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

16 Samples

Download data: TXT

(Submitter supplied) RNA-seq analysis of N-acetylmannosamine upon growth and gene expression in pneumococci grown with galactose as sole sugar.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22606

12 Samples

Download data: TXT

(Submitter supplied) Streptococcus pneumoniae is the leading cause of community-acquired pneumonia. The release of the pore-forming cholesterol-dependent cytolysin (CDC) pneumolysin (PLY) and hydrogen peroxide (H2O2), a physiological metabolite, is an important virulence determinant of pneumococci.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23797

10 Samples

Download data: TXT

(Submitter supplied) Strain ∆staRPZnstaR was obtained to investigate genome-wide differential gene expresion when staR gene is reppressed or overexpressed by adding Zn to the culture medium. In addition, gene expression profile alterations produced upon inhibition of gyrase with Novobiocin were studied in the absence and presence of StaR.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

8 Samples

Download data: TXT

(Submitter supplied) Deep sequencing of cDNA from Streptococcus pneumoniae EF3030 bacteria and normalized human broncial epithelial cells (nHBEC), alone, together, and after coincubation with Influenza A virus pH1N1.

Organism:

Influenza A virus; Streptococcus pneumoniae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

5 related Platforms

14 Samples

Download data: MATRIX

(Submitter supplied) This study investigates the global transcriptional response of Streptococcus pneumoniae D39V (serotype 2 strain) to human blood components and CSF acquired from discarded and anonymized patient samples.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

30 Samples

Download data: CSV, GFF3

(Submitter supplied) Here we analyze the transcriptomic profile of Streptococcus pneumoniae D39 Dspd_1448::KanrpsL (IU10991) vs Streptcococcus penumoiae D39 wt (IU1781) under anaerobic growth condition in BHI growth media

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24384

6 Samples

Download data: TXT

(Submitter supplied) In our study, deletion of methionine synthesis gene metE (SPD_0510) impaired the growth of Streptococcus pneumoniae strain D39 significantly when bacteria were cultured in chemically defined medium with a low concentration of methionine. Bacteria undergo methionine starvation in this condition. We aimed to know how S. pneumoniae responses to methionine starvation, particularly at the transcriptional level. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

4 Samples

Download data: XLSX

(Submitter supplied) This study reports on the co-administration of a zinc ionophore (PBT-2) and ampicillin to break antibiotic resistance of Streptococcus pneumoniae in a murine pneumonia model. The molecular mechanism for this heightened antimicrobial activity was identified through transcriptomic and metabolomic analyses of a wild type strain and a zinc efflux mutant to identify cellular targets of zinc intoxication. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23152

3 Samples

Download data: TXT, XLSX

(Submitter supplied) Streptococcus pneumoniae strain D39V were grown in six different conditions, mimicking the host niches

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26308

18 Samples

Download data: TXT

(Submitter supplied) Zebrafish embryo has been emerging as an interesting model of infection due to their fecundity, transparency and availability of genetic tools. Streptococcus pneumoniae, the pneumococcus, is the main etiological agent of pneumonia, sepsis and meningitis. The bacteria expresses a very important virulence factor, pneumolysin able to form pores on cholesterol-based membranes and to activate innate immune system. more...

Organism:

Danio rerio; Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25946

6 Samples

Download data: TXT

(Submitter supplied) During its progression from nasopharynx to other sterile and non-sterile niches of its human host, Streptococcus pneumoniae must cope with changes in temperature. We hypothesized that the pneumococcal temperature adaptation is an important facet of pneumococcal in host survival. Here we evaluate the effect of temperature on the phenotype of pneumococcus and the role of glutamate dehydrogenase (GdhA) during thermal stress adaptation associated with virulence and survival. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL22631

8 Samples

Download data: TXT

(Submitter supplied) Transcriptomic analysis of Streptococcus pneumoniae TIGR4 wildtype and it's isogenic ccpA mutant, grown in chemically defined media supplemented with physiological levels of carbohydrates to mimic the human nasopharynx and bloodstream. The goal is to examine how anatomical site-specific carbohydrate availability impacts S. pneumoniae physiology and virulence.

Organism:

Streptococcus pneumoniae TIGR4

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30972

10 Samples

Download data: TXT

(Submitter supplied) Streptococcus pneumoniae is the primary cause of community-acquired bacterial pneumonia with rates of penicillin and multi-drug resistance exceeding 80% and 40%, respectively. The innate immune response uses various chemical insults to control infection, including metal stress mediated by localized changes in zinc abundance. Here, we characterized the impact of S. pneumoniae zinc intoxication revealing disruptions in central carbon metabolism, lipid biogenesis and peptidoglycan biosynthesis. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23152

6 Samples

Download data: XLSX