GEO DataSets

(Submitter supplied) K78 acetylation inhibits the global outcome of DasR binding to its targeted genes in vivo.

Organism:

Saccharopolyspora erythraea

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33125

4 Samples

Download data: BW

(Submitter supplied) C-di-AMP is primarily associated with the regulation of carbon utilization as well as other central traits, central metabolism, and bacterial stringent response to environmental changes. Elevated c-di-AMP levels result in aberrant physiology for most c-di-AMP synthesizing organisms, drawing particular attention to the importance of the c-di-AMP homeostasis and the molecular mechanisms pertaining to nucleotide metabolism and signal transduction. more...

Organism:

Saccharopolyspora erythraea

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33125

4 Samples

Download data: TDF

(Submitter supplied) Actinobacteria are ubiquitous bacteria undergoing complex developmental transition coincided with antibiotics production in response to stress or nutrients starvation. This transition is mainly controlled by the interaction between the second messenger c-di-GMP and the master repressor BldD. To date, the upstream factors and the global signal networks that regulate this intriguing cell biological processes still remained unknown. more...

Organism:

Saccharopolyspora erythraea

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33125

4 Samples

Download data: BW

(Submitter supplied) Omics approaches have succeeded in understanding the boosted productivity of natural products by industrial high-producing microorganisms. Here, with the updated genome sequence and transcriptomic profiles derived from next-generation high throughput sequencing, we exploited comparative omics analysis to further enhance the biosynthesis of erythromycin in an industrial overproducer, Saccharopolyspora erythraea HL3168 E3. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28565

4 Samples

Download data: XLSX

(Submitter supplied) Knowledge about effects of cofactor perturbation on cellular metabolism is scarce with respect to Saccharopolyspora erythraea. The water-forming NADH oxidase (NOX) from Streptococcus pneumonia was expressed in S.erythraea E3, an important industrial strain for erythromycin production, at three different levels to investigate effects of intracellular redox status on secondary metabolism. NOX expression reduced the intracellular [NADH]/[NAD+] ratios significantly, although with a strong constitutive promoter NOX function was limited due to the shortage of oxygen. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26951

6 Samples

Download data: TSV

(Submitter supplied) Saccharopolyspora erythraea is used for industrial-scale production of erythromycin. To explore the physiological role of co-factors in regulation of primary and secondary metabolism of S. erythraea, we initially overexpressed the endogenous F1-ATPase in an erythromycin high-producing strain, E3. The engineered strain is named EA. The F1-ATPase expression resulted in a lower [ATP]/[ADP] ratio, which was accompanied by a dramatic increased production of a reddish pigment and a decreased erythromycin production. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26951

8 Samples

Download data: TSV

(Submitter supplied) Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation.

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by array

Platform:

GPL19656

22 Samples

Download data: TXT

(Submitter supplied) Transcription profile analysis between the wild-type strain NRRL23338 and the dasR null mutant to determine the overall transcription changes caused by the deletion of global regulatory gene dasR in S.erythraea.

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by array

Platform:

GPL20270

4 Samples

Download data: TXT

(Submitter supplied) We report the effect of addition of N-acetylglucosamine in Saccharopolyspora erythraea reactor cultures on the transcription of genes related to dasR regulon

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15864

3 Samples

Download data: FPKM_TRACKING

(Submitter supplied) We report the high-throughput profiling of saccharopolyspora erythraea including a industrial strain HL3168 E3 and a wild-type strain NRRL23338. The aim was to evaluate the difference in expression of sRNA predicted in silico related to secondary metabolites in Saccharopolyspora erythraea.

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17450

2 Samples

Download data: TXT

(Submitter supplied) We report the high-throughput profiling of saccharopolyspora erythraea life cycle in bioreactors. RNA was harvested at seven time-points from bioreactors, three points immediately before, one during and three following the metabolic switch (as evidenced by biomass cell dry weight). We prepared unstranded RNA libraries and sequenced 363 million reads for a total of 32.7Gb and a mean ~4,000 fold coverage of the genome. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15864

7 Samples

Download data: TXT

(Submitter supplied) Information about molecular mechanisms underlying improvement of antibiotic-producing microorganisms with traditional mutation and screening approach is largely missing. This information is essential to develop rational approaches to strain improvement. In this study by using comparative genomic analysis we have identified all genetic changes that have occurred during development of an erythromycin overproducer, which was obtained by the traditional mutate-and-screen method. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by array

Platform:

GPL6051

22 Samples

Download data: CEL, CHP

(Submitter supplied) In this study we have used the rifampicin selection as a tool to genetically improve the erythromycin producer Saccharopolyspora erythraea. Two rifampicin-resistant (rif) mutants, rif1 and rif6, have been characterized in more detail. With respect to the parental strain NRRL2338, rif1 (harboring the missense S444F) exhibited higher respiratory performance and final erythromycin yields; in contrast, rif6 (harboring the missense Q426R) was slow-growing, developmental-defective and severely impaired in erythromycin production. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by array

Platform:

GPL6051

12 Samples

Download data: CEL, XML

(Submitter supplied) A DNA microarray was designed and constructed using the genome sequence of Saccharopolyspora erythraea strain NRRL 2338. Following growth in liquid medium, we analysed the expression of 6494 ORFs along the time course. The results indicated that the 404 genes, whose expression significatively correlated with the time course, identify three distinct growth phases: a rapid growth until 32 h (phase A); a growth slowdown until 52 h (phase B); another rapid growth phase from 56 h to 72 h (phase C) before entering the stationary phase. more...

Organism:

Saccharopolyspora erythraea

Type:

Expression profiling by array

Platform:

GPL6051

20 Samples

Download data: CEL

(Submitter supplied) Saccharopolyspora erythraea transcriptome array (UniCam sequence + SNP info) Arrays of this design have barcodes that begin with 16042341 or 2542341. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. more...

Organism:

Saccharopolyspora erythraea

1 Series

22 Samples

Download data: TXT

(Submitter supplied) see manufacturer's web site at http://www.agilent.com/

Organism:

Saccharopolyspora erythraea

1 Series

4 Samples

Download data