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CWC25 U2-type spliceosomal complex subunit CWC25 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855476, updated on 4-Jan-2025

Summary

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Official Symbol
CWC25
Official Full Name
U2-type spliceosomal complex subunit CWC25
Primary source
SGD:S000005189
Locus tag
YNL245C
See related
AllianceGenome:SGD:S000005189; FungiDB:YNL245C; VEuPathDB:YNL245C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in mRNA splicing, via spliceosome. Part of U2-type spliceosomal complex. Orthologous to human CWC25 (CWC25 spliceosome associated protein homolog). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See CWC25 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
1
Sequence:
Chromosome: XIV; NC_001146.8 (186346..186885, complement)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene DNA-directed RNA polymerase I subunit RPA49 Neighboring gene cysteine--tRNA ligase Neighboring gene Vps75p Neighboring gene translation initiation factor eIF1 Neighboring gene Sla2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing. Krishnan R, et al. Nat Struct Mol Biol, 2013 Dec. PMID 24240612, Free PMC Article
  2. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. Tseng CK, et al. RNA, 2011 Jan. PMID 21098140, Free PMC Article
  3. Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Warkocki Z, et al. Nat Struct Mol Biol, 2009 Dec. PMID 19935684
  4. Structure of a yeast step II catalytically activated spliceosome. Yan C, et al. Science, 2017 Jan 13. PMID 27980089
  5. A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing. Tseng CK, et al. RNA, 2017 Apr. PMID 28057857, Free PMC Article

See all (39) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In the process of pre-mRNA splicing, Cwc25 binds tightly to the spliceosome after the reaction and is then removed from the spliceosome, which normally requires Prp16 and ATP hydrolysis, to allow the occurrence of the second reaction.
    Title: A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.
  2. Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed.
    Title: Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.
  3. Cwc25 functions in the final step of the first catalytic reaction after the action of Prp2.
    Title: Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of U2-type spliceosomal complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
U2-type spliceosomal complex subunit CWC25
NP_014154.1
  • Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    186346..186885 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183083.1 → NP_014154.1  TPA: U2-type spliceosomal complex subunit CWC25 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014154.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W0U8, P53854
    UniProtKB/TrEMBL
    B3LP84, B5VQG3, C7GPB6, G2WLM1, N1NZZ3
    Conserved Domains (2) summary
    smart01083
    Location:11 → 47
    Cir_N; N-terminal domain of CBF1 interacting co-repressor CIR
    pfam12542
    Location:74 → 153
    CWC25; Pre-mRNA splicing factor
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P53854.1

Gene LinkOut

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