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NOP2 rRNA (cytosine-C5-)-methyltransferase NOP2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855664, updated on 4-Jan-2025

Summary

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Official Symbol
NOP2
Official Full Name
rRNA (cytosine-C5-)-methyltransferase NOP2
Primary source
SGD:S000005005
Locus tag
YNL061W
See related
AllianceGenome:SGD:S000005005; FungiDB:YNL061W; VEuPathDB:YNL061W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
YNA1
Summary
Enables rRNA (cytosine-C5-)-methyltransferase activity. Involved in assembly of large subunit precursor of preribosome; maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); and rRNA base methylation. Located in nucleolus. Part of preribosome, large subunit precursor. Used to study cancer. Orthologous to human NOP2 (NOP2 nucleolar protein). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See NOP2 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
1
Sequence:
Chromosome: XIV; NC_001146.8 (510540..512396)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene S-adenosylmethionine-dependent methyltransferase Neighboring gene tRNA 1-methyladenosine methyltransferase subunit GCD10 Neighboring gene Arp5p Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker. de Beus E, et al. J Cell Biol, 1994 Dec. PMID 7806561, Free PMC Article
  2. Nop2p is required for pre-rRNA processing and 60S ribosome subunit synthesis in yeast. Hong B, et al. Mol Cell Biol, 1997 Jan. PMID 8972218, Free PMC Article
  3. A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue. King M, et al. Biochem J, 1999 Jan 1. PMID 9854021, Free PMC Article
  4. Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae. Hong B, et al. Nucleic Acids Res, 2001 Jul 15. PMID 11452018, Free PMC Article
  5. RNA methyltransferases utilize two cysteine residues in the formation of 5-methylcytosine. King MY, et al. Biochemistry, 2002 Sep 17. PMID 12220187

See all (133) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Nop2 belongs to the large family of pre-ribosomal proteins and possesses two related functions in pre-rRNA processing: as an essential factor for cleavages and 5-methylcytosine : RNA : modification.
    Title: Eukaryotic rRNA Modification by Yeast 5-Methylcytosine-Methyltransferases and Human Proliferation-Associated Antigen p120.
  2. Nop2 catalyzes the methylation of m5C2870 in 25S rRNA.
    Title: Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables S-adenosylmethionine-dependent methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (cytosine-C5-)-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (cytosine-C5-)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA (cytosine-C5-)-methyltransferase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables rRNA (cytosine-C5-)-methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in assembly of large subunit precursor of preribosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in maturation of LSU-rRNA IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA base methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rRNA base methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in rRNA base methylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in rRNA base methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosomal large subunit biogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in nucleolus HDA PubMed 
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of preribosome, large subunit precursor HDA PubMed 
part_of preribosome, large subunit precursor IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
rRNA (cytosine-C5-)-methyltransferase NOP2
NP_014338.3
  • rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    510540..512396
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182899.3 → NP_014338.3  TPA: rRNA (cytosine-C5-)-methyltransferase NOP2 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014338.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W1B9, E9P914, P40991
    UniProtKB/TrEMBL
    A6ZS19, B3LNS0, B5VQY3, C7GLU2, G2WM44, N1NZI4
    Conserved Domains (1) summary
    TIGR00446
    Location:273 → 548
    nop2p; NOL1/NOP2/sun family putative RNA methylase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P40991.1

Gene LinkOut

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