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thiI tRNA uridine 4-sulfurtransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945067, updated on 17-Dec-2024

Summary

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Official Symbol
thiI
Official Full Name
tRNA uridine 4-sulfurtransferase
Primary source
ECOCYC:G6238
Locus tag
b0423
See related
ASAP:ABE-0001470
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0417; nuvA; yajK
Summary
The 4-thiouridine modification of tRNA acts as a near UV sensor because it makes tRNA near-UV labile, blocking translation, inducing the stringent response and stopping growth. [More information is available at EcoGene: EG13273]. ThiI is required for the synthesis of the thiazole moiety of thiamine and plays a role in the conversion of uridine to 4-thiouridine at position 8 in tRNA . [More information is available at EcoCyc: G6238].
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Genomic context

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Sequence:
NC_000913.3 (441549..442997)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene geranyl diphosphate/farnesyl diphosphate synthase Neighboring gene exodeoxyribonuclease VII subunit XseB Neighboring gene protein/nucleic acid deglycase 3 Neighboring gene 2-dehydropantoate 2-reductase Neighboring gene nucleotide binding protein YajQ

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Evidence that ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis, may be a sulfurtransferase that proceeds through a persulfide intermediate. Palenchar PM, et al. J Biol Chem, 2000 Mar 24. PMID 10722656
  2. Enzymes producing 4-thiouridine in Escherichia coli tRNA: approximate chromosomal locations of the genes and enzyme activities in a 4-thiouridine-deficient mutant. Lipsett MN. J Bacteriol, 1978 Sep. PMID 357427, Free PMC Article
  3. Isolation of single-site Escherichia coli mutants deficient in thiamine and 4-thiouridine syntheses: identification of a nuvC mutant. Ryals J, et al. J Bacteriol, 1982 Aug. PMID 6178725, Free PMC Article
  4. 4-Thiouridine triggers both growth delay induced by near-ultraviolet light and photoprotection. Thomas G, et al. Eur J Biochem, 1980 Dec. PMID 7007049
  5. Using genomic information to investigate the function of ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis. Mueller EG, et al. Protein Sci, 1999 Nov. PMID 10595545, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables CCA tRNA nucleotidyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sulfur carrier activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sulfurtransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sulfurtransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables tRNA-uracil-4 sulfurtransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-cysteine catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in detection of UV NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in tRNA 4-thiouridine biosynthesis EXP
Inferred from Experiment
more info
 PubMed 
involved_in tRNA 4-thiouridine biosynthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA 4-thiouridine biosynthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA thio-modification IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in thiamine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiazole biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in thiazole biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of L-cysteine desulfurase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of sulfurtransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
tRNA uridine 4-sulfurtransferase
NP_414957.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    441549..442997
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414957.1 tRNA uridine 4-sulfurtransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414957.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1V2GJV7
    Conserved Domains (1) summary
    PRK01269
    Location:1 → 482
    PRK01269; tRNA s(4)U8 sulfurtransferase; Provisional
Nucleotide Protein
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