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nth endonuclease III [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947122, updated on 3-Dec-2024

Summary

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Official Symbol
nth
Official Full Name
endonuclease III
Primary source
ECOCYC:EG10662
Locus tag
b1633
See related
ASAP:ABE-0005463
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1629
Summary
Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].
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Genomic context

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Sequence:
NC_000913.3 (1711523..1712158)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene SoxR [2Fe-2S] reducing system protein RsxD Neighboring gene SoxR [2Fe-2S] reducing system protein RsxG Neighboring gene SoxR [2Fe-2S] reducing system protein RsxE Neighboring gene dipeptide/tripeptide:H(+) symporter DtpA Neighboring gene glutathione S-transferase GstA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Role of endonuclease III enzymes in uracil repair. Yang Y, et al. Mutat Res, 2019 Jan. PMID 30590231, Free PMC Article
  2. Novel substrates of Escherichia coli nth protein and its kinetics for excision of modified bases from DNA damaged by free radicals. Dizdaroglu M, et al. Biochemistry, 2000 May 9. PMID 10820032
  3. Enzymatic processing of uracil glycol, a major oxidative product of DNA cytosine. Purmal AA, et al. J Biol Chem, 1998 Apr 17. PMID 9545349
  4. Excision of oxidative cytosine modifications from gamma-irradiated DNA by Escherichia coli endonuclease III and human whole-cell extracts. Wagner JR, et al. Anal Biochem, 1996 Jan 1. PMID 8789150
  5. In vitro studies on DNA-photosensitization by different drug stereoisomers. Lhiaubet-Vallet V, et al. Toxicol In Vitro, 2003 Oct-Dec. PMID 14599458

See all (73) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. WT NTHL1 (human) and Nth (E. coli) are remarkably alike with respect to specificity of glycosylase reaction, and although NTHL1 is a much slower enzyme than Nth, the tighter binding of NTHL1 compensates, resulting in similar kcat/Kd values for both enzymes with each of the substrates tested. EcoNth Thr121 residue influences enzyme binding to DNA, as binding is significantly reduced with the Thr121Ala variant.
    Title: Probing the activity of NTHL1 orthologs by targeting conserved amino acid residues.
  2. A possible pathway is proposed through which charge can travel between the DNA and the iron sulfur cluster of EndoIII, as a first step in damage detection.
    Title: Mutants of the base excision repair glycosylase, endonuclease III: DNA charge transport as a first step in lesion detection.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene.
  4. Endonuclease III does not protect E.coli against possible mutations attributable to formamidopyrimidine lesions.
    Title: Excision of formamidopyrimidine lesions by endonucleases III and VIII is not a major DNA repair pathway in Escherichia coli.
  5. Electrochemical measurement of endonuclease III using DNA-modified electrodes demonstrates that this base excision repair enzyme containing a [4Fe-4S] cofactor is redox-active when bound to DNA.
    Title: DNA-bound redox activity of DNA repair glycosylases containing [4Fe-4S] clusters.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA N-glycosylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables uracil DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair, AP site formation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within base-excision repair, AP site formation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within base-excision repair, AP site formation via deaminated base removal IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within cellular response to UV IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to stress IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
endonuclease III

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1711523..1712158
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416150.1 endonuclease III [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416150.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AID0
    Conserved Domains (1) summary
    PRK10702
    Location:1 → 211
    PRK10702; endonuclease III; Provisional
Nucleotide Protein
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