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phoU negative regulator of the pho regulon [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948233, updated on 3-Dec-2024

Summary

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Official Symbol
phoU
Official Full Name
negative regulator of the pho regulon
Primary source
ECOCYC:EG10735
Locus tag
b3724
See related
ASAP:ABE-0012177
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3717; phoT; pst
Summary
postulated that phoU encoded a repressor or a subunit of repressor for PhoB or one of the enzymes for converting phosphate to the hypothetical effector (corepressor), which activates a repressor for phoB . [More information is available at EcoCyc: EG10735].
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Genomic context

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Sequence:
NC_000913.3 (3906853..3907578, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene beta-glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase Neighboring gene transcriptional antiterminator BglG Neighboring gene phosphate ABC transporter ATP binding subunit Neighboring gene phosphate ABC transporter membrane subunit PstA Neighboring gene phosphate ABC transporter membrane subunit PstC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Role of PhoU in phosphate transport and alkaline phosphatase regulation. Muda M, et al. J Bacteriol, 1992 Dec. PMID 1459954, Free PMC Article
  2. PhoU enhances the ability of extraintestinal pathogenic Escherichia coli strain CFT073 to colonize the murine urinary tract. Buckles EL, et al. Microbiology (Reading), 2006 Jan. PMID 16385125
  3. Regulation of the phosphate regulon in Escherichia coli K-12: regulation of the negative regulatory gene phoU and identification of the gene product. Nakata A, et al. J Bacteriol, 1984 Sep. PMID 6090402, Free PMC Article
  4. Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12. Surin BP, et al. J Bacteriol, 1986 Nov. PMID 3536855, Free PMC Article
  5. PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli. Li Y, et al. Antimicrob Agents Chemother, 2007 Jun. PMID 17420206, Free PMC Article

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PhoU interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
    Title: The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
  2. The results indicate that PhoU controls the activity of the PstSCAB(2) transporter, as well as its abundance within the cell.
    Title: Employment of a promoter-swapping technique shows that PhoU modulates the activity of the PstSCAB2 ABC transporter in Escherichia coli.
  3. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein.
    Title: Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli.
  4. N-terminus verified by Edman degradation on complete protein
    Title: Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12.
  5. newly indentified persistence switch in persister formation and tolerance to antibiotics and stresses.
    Title: PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in cellular response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to phosphate starvation IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular response to starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular phosphate ion homeostasis IEA
Inferred from Electronic Annotation
more info
  
involved_in intracellular phosphate ion homeostasis TAS
Traceable Author Statement
more info
 PubMed 
involved_in negative regulation of gene expression IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of gene expression TAS
Traceable Author Statement
more info
 PubMed 
involved_in negative regulation of phosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of phosphate metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of phosphate metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of phosphate transmembrane transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phosphate ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
negative regulator of the pho regulon

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3906853..3907578 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418180.1 negative regulator of the pho regulon [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418180.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S0FF14
    Conserved Domains (1) summary
    PRK11115
    Location:1 → 236
    PRK11115; phosphate signaling complex protein PhoU
Nucleotide Protein
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